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Nagata R, Kawachi E, Hashimoto Y, Shudo K: Cytokinin-specific binding protein in etiolated mung bean seedlings. Biochem Biophys Res Commun. 1993 Mar 15;191(2):543-9.
We have found that a synthetic urea derivative, N-(2-chloro-4-pyridyl)-N'-phenylurea (4PU30, forchlorfenuron), has even higher cytokinin activity than N6-benzyladenine (BA).
Using [3H] 4PU30 (2.83 TBq/mmol) as a probe, we confirmed chromatographically the presence of a high-affinity cytokinin-specific binding protein (CSBP) in whole-cell extract from etiolated mung bean seedlings. |
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