Name | beta glucuronidase |
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Synonyms | Beta glucuronidase; Beta G1; Beta glucuronidase precursor; GUSB; MPS 7; MPS7; Beta G1s; Beta glucuronidase precursors |
Name | kinetin |
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CAS |
PubMed | Abstract | RScore(About this table) | |
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16652220 | Sudan C, Prakash S, Bhomkar P, Jain S, Bhalla-Sarin N: Ubiquitous presence of beta-glucuronidase (GUS) in plants and its regulation in some model plants. Planta. 2006 Sep;224(4):853-64. Epub 2006 Apr 28. |
2(0,0,0,2) | Details |
15133712 | Manickavasagam M, Ganapathi A, Anbazhagan VR, Sudhakar B, Selvaraj N, Vasudevan A, Kasthurirengan S: Agrobacterium-mediated genetic transformation and development of herbicide-resistant sugarcane species hybrids) using axillary buds. Plant Cell Rep. 2004 Sep;23(3):134-43. Epub 2004 May 5. Axillary bud explants from 6-month-old sugarcane cultivars Co92061 and Co671 were co-cultivated with Agrobacterium strains LBA4404 and EHA105 that harboured a binary vector pGA492 carrying neomycin phosphotransferase II, phosphinothricin acetyltransferase (bar) and an intron containing beta-glucuronidase (gus-intron) genes in the T-DNA region. Transgenic plants were generated in three different steps: (1) production of putative primary transgenic shoots in Murashige-Skoog (MS) liquid medium with 3.0 mg l (-1) 6-benzyladenine (BA) and 5.0 mg l (-1) PPT, (2) production of secondary transgenic shoots from the primary transgenic shoots by growing them in MS liquid medium with 2.0 mg l (-1) BA, 1.0 mg l (-1) kinetin (Kin), 0.5 mg l (-1) alpha-napthaleneacetic acid (NAA) and 5.0 mg l (-1) PPT for 3 weeks, followed by five more cycles of shoot proliferation and selection under same conditions, and (3) rooting of transgenic shoots on half-strength MS liquid medium with 0.5 mg l (-1) NAA and 5.0 mg l (-1) PPT. |
2(0,0,0,2) | Details |
17593368 | Arockiasamy S, Ignacimuthu S: Regeneration of transgenic plants from two indica rice (Oryza sativa L.) cultivars using shoot apex explants. Plant Cell Rep. 2007 Oct;26(10):1745-53. Epub 2007 Jun 26. The vector contained an improved hygromycin phosphotransferase (hpt) gene for hygromycin resistance driven by actin 1 promoter and the reporter gene beta-glucuronidase intron (INT-GUS) controlled by CaMV 35S promoter. Rice shoots were induced on media containing 0.1 mg/l napthalene (NAA), 1.0 mg/l kinetin (kn), 1.0 mg/l N (6)-benzyleaminopurin (BAP), 300 mg/l casaminoacid, 500 mg/ 50 mg/l hygromycin and 500 mg/l cefotaxime. |
1(0,0,0,1) | Details |
16113211 | Welchen E, Gonzalez DH: Differential expression of the Arabidopsis cytochrome c genes Cytc-1 and Cytc-2. Plant Physiol. 2005 Sep;139(1):88-100. Epub 2005 Aug 19. The promoters of the Arabidopsis (Arabidopsis thaliana) cytochrome c genes, Cytc-1 and Cytc-2, were analyzed using plants transformed with fusions to the beta-glucuronidase coding sequence. |
1(0,0,0,1) | Details |
12207665 | Samanani N, Park SU, Facchini PJ: In vitro regeneration and genetic transformation of the The cotyledons were cultured on a shoot induction medium (B5 salts and vitamins, 30 g l-1 2 mg l-1 kinetin, and 3 g l-1 Gelrite) containing 25 mg l-1 hygromycin B as the selection agent and 250 mg l-1 timentin to facilitate the elimination of Agrobacterium. Detection of the beta-glucuronidase (GUS) gene using a polymerase chain reaction assay, the high levels of GUS mRNA and enzyme activity, and the cytohistochemical localization of GUS activity confirmed the genetic transformation of callus cultures and regenerated plants. |
-producing plant, Thalictrum flavum ssp. glaucum. Physiol Plant. 2002 Sep;116(1):79-86.1(0,0,0,1) | Details |
10608655 | Yi HC, Joo S, Nam KH, Lee JS, Kang BG, Kim WT: Auxin and brassinosteroid differentially regulate the expression of three members of the 1-aminocyclopropane-1-carboxylate synthase gene family in mung bean (Vigna radiata L.). Plant Mol Biol. 1999 Nov;41(4):443-54. In addition, BR synergistically increased the IAA-induced VR-ACS6 and VR-ACS7 transcript levels, while it effectively abolished both the IAA- and kinetin-induced accumulation of VR-ACS1 mRNA. The VR-ACS7 gene was isolated, and chimeric fusion between the 2.4 kb 5'-upstream region and the beta-glucuronidase (GUS) reporter gene was constructed and introduced into Nicotiana tabacum. |
1(0,0,0,1) | Details |
14551734 | Jayashree R, Rekha K, Venkatachalam P, Uratsu SL, Dandekar AM, Kumari Jayasree P, Kala RG, Priya P, Sushma Kumari S, Sobha S, Ashokan MP, Sethuraj MR, Thulaseedharan A: Genetic transformation and regeneration of rubber tree (Hevea brasiliensis Muell. Plant Cell Rep. 2003 Oct;22(3):201-9. Epub 2003 Jul 9. Mature embryos were germinated and developed into plantlets on MS4 medium supplemented with 0.2 mg l (-1) 0.2 mg l (-1) kinetin (KIN) and 0.1 mg l (-1) The beta-glucuronidase gene (uidA) was used for screening and the neomycin phosphotransferase gene (nptII) was used for selection of the transformed calli. |
1(0,0,0,1) | Details |
15815929 | Saini R, Jaiwal PK: Transformation of a recalcitrant grain legume, Vigna mungo L. Plant Cell Rep. 2005 Jun;24(3):164-71. Epub 2005 Apr 7. Hepper transformation was significantly increased from an average of 1% to 6.5% by using shoot apices excised from embryonic axes precultured on 10 microM benzyl- (BAP) for 3 days and wounded prior to inoculation in Agrobacterium tumefaciens strain EHA105 carrying the binary vector pCAMBIA2301, which contains a neomycin phosphotransferase gene (nptII) and a beta-glucuronidase (GUS) gene (gusA) interrupted by an intron. |
1(0,0,0,1) | Details |
19655147 | Wu AM, Hu JS, Liu JY: Functional analysis of a cotton cellulose synthase A4 gene promoter in transgenic tobacco plants. Plant Cell Rep. 2009 Oct;28(10):1539-48. Epub 2009 Aug 5. A 1,482-bp promoter sequence of the cotton cellulose synthase gene (GhCesA4) was isolated from Chinese cultivar CRI12 of Gossypium hirsutum, and transcriptionally fused to a beta-glucuronidase (GUS) reporter gene for investigation of important regions controlling gene expression in transgenic tobacco plants. Exposure of the transgenic tobacco to various abiotic stresses showed that the full-length construct predominantly responded to NAA, kinetin, and sugar. |
1(0,0,0,1) | Details |
7697294 | Yabe N, Takahashi T, Komeda Y: Analysis of tissue-specific expression of Arabidopsis thaliana HSP90-family gene HSP81. Plant Cell Physiol. 1994 Dec;35(8):1207-19. In situ hybridization and histochemical analysis using transgenic plants carrying chimeric gene fusions, with an HSP81 promoter region fused to a beta-glucuronidase (GUS) gene, confirmed these results. Exogenous application of various chemicals such as ABA, kinetin, IAA, NaCl, and revealed that 10 mM IAA and 0.1 M NaCl significantly enhanced the accumulation of HSP81-2 and -3 transcripts. |
1(0,0,0,1) | Details |
18057938 | Facchini PJ, Loukanina N, Blanche V: Genetic transformation via somatic embryogenesis to establish herbicide-resistant opium poppy. Plant Cell Rep. 2008 Apr;27(4):719-27. Epub 2007 Dec 5. Transformation was mediated by Agrobacterium tumefaciens using the pCAMBIA3301 vector, which harbors the phosphinothricin acetyltransferase (pat) gene driven by a tandem repeat of the cauliflower mosaic virus (CaMV) 35S promoter and the beta-glucuronidase (gus) structural gene driven by a single copy of the CaMV 35S promoter between left- and right-border sequences. |
1(0,0,0,1) | Details |
8453300 | Uknes S, Dincher S, Friedrich L, Negrotto D, Williams S, Thompson-Taylor H, Potter S, Ward E, Ryals J: Regulation of pathogenesis-related protein-1a gene expression in tobacco. Plant Cell. 1993 Feb;5(2):159-69. Several artifacts, most importantly ectopic expression in pollen, were associated with the use of the beta-glucuronidase reporter gene. |
1(0,0,0,1) | Details |
18335952 | O'Neill KM, Larsen JS, Curtis WR: Scale-up of Agrobacterium-mediated transient protein expression in bioreactor-grown Nicotiana glutinosa plant cell suspension culture. Biotechnol Prog. 2008 Mar-Apr;24(2):372-6. Epub 2008 Mar 12. The reporter gene beta-glucuronidase was transiently expressed in a 51-L bioreactor-grown plant cell suspension culture of Nicotiana glutinosa at a yield of approximately 1.1 mg through co-culture with an auxotrophic strain of Agrobacterium tumefaciens. |
1(0,0,0,1) | Details |