Protein Information

Name beta glucuronidase
Synonyms Beta glucuronidase; Beta G1; Beta glucuronidase precursor; GUSB; MPS 7; MPS7; Beta G1s; Beta glucuronidase precursors

Compound Information

Name kinetin
CAS

Reference List

PubMed Abstract RScore(About this table)
16652220 Sudan C, Prakash S, Bhomkar P, Jain S, Bhalla-Sarin N: Ubiquitous presence of beta-glucuronidase (GUS) in plants and its regulation in some model plants. Planta. 2006 Sep;224(4):853-64. Epub 2006 Apr 28.

2(0,0,0,2) Details
15133712 Manickavasagam M, Ganapathi A, Anbazhagan VR, Sudhakar B, Selvaraj N, Vasudevan A, Kasthurirengan S: Agrobacterium-mediated genetic transformation and development of herbicide-resistant sugarcane (Saccharum species hybrids) using axillary buds. Plant Cell Rep. 2004 Sep;23(3):134-43. Epub 2004 May 5.

Axillary bud explants from 6-month-old sugarcane cultivars Co92061 and Co671 were co-cultivated with Agrobacterium strains LBA4404 and EHA105 that harboured a binary vector pGA492 carrying neomycin phosphotransferase II, phosphinothricin acetyltransferase (bar) and an intron containing beta-glucuronidase (gus-intron) genes in the T-DNA region.
Transgenic plants were generated in three different steps: (1) production of putative primary transgenic shoots in Murashige-Skoog (MS) liquid medium with 3.0 mg l (-1) 6-benzyladenine (BA) and 5.0 mg l (-1) PPT, (2) production of secondary transgenic shoots from the primary transgenic shoots by growing them in MS liquid medium with 2.0 mg l (-1) BA, 1.0 mg l (-1) kinetin (Kin), 0.5 mg l (-1) alpha-napthaleneacetic acid (NAA) and 5.0 mg l (-1) PPT for 3 weeks, followed by five more cycles of shoot proliferation and selection under same conditions, and (3) rooting of transgenic shoots on half-strength MS liquid medium with 0.5 mg l (-1) NAA and 5.0 mg l (-1) PPT.
2(0,0,0,2) Details
17593368 Arockiasamy S, Ignacimuthu S: Regeneration of transgenic plants from two indica rice (Oryza sativa L.) cultivars using shoot apex explants. Plant Cell Rep. 2007 Oct;26(10):1745-53. Epub 2007 Jun 26.

The vector contained an improved hygromycin phosphotransferase (hpt) gene for hygromycin resistance driven by actin 1 promoter and the reporter gene beta-glucuronidase intron (INT-GUS) controlled by CaMV 35S promoter.
Rice shoots were induced on media containing 0.1 mg/l napthalene acetic acid (NAA), 1.0 mg/l kinetin (kn), 1.0 mg/l N (6)-benzyleaminopurin (BAP), 300 mg/l casaminoacid, 500 mg/l proline, 50 mg/l hygromycin and 500 mg/l cefotaxime.
1(0,0,0,1) Details
16113211 Welchen E, Gonzalez DH: Differential expression of the Arabidopsis cytochrome c genes Cytc-1 and Cytc-2. Plant Physiol. 2005 Sep;139(1):88-100. Epub 2005 Aug 19.


The promoters of the Arabidopsis (Arabidopsis thaliana) cytochrome c genes, Cytc-1 and Cytc-2, were analyzed using plants transformed with fusions to the beta-glucuronidase coding sequence.
1(0,0,0,1) Details
12207665 Samanani N, Park SU, Facchini PJ: In vitro regeneration and genetic transformation of the berberine-producing plant, Thalictrum flavum ssp. glaucum. Physiol Plant. 2002 Sep;116(1):79-86.

The cotyledons were cultured on a shoot induction medium (B5 salts and vitamins, 30 g l-1 sucrose, 2 mg l-1 kinetin, and 3 g l-1 Gelrite) containing 25 mg l-1 hygromycin B as the selection agent and 250 mg l-1 timentin to facilitate the elimination of Agrobacterium.
Detection of the beta-glucuronidase (GUS) gene using a polymerase chain reaction assay, the high levels of GUS mRNA and enzyme activity, and the cytohistochemical localization of GUS activity confirmed the genetic transformation of callus cultures and regenerated plants.
1(0,0,0,1) Details
10608655 Yi HC, Joo S, Nam KH, Lee JS, Kang BG, Kim WT: Auxin and brassinosteroid differentially regulate the expression of three members of the 1-aminocyclopropane-1-carboxylate synthase gene family in mung bean (Vigna radiata L.). Plant Mol Biol. 1999 Nov;41(4):443-54.

In addition, BR synergistically increased the IAA-induced VR-ACS6 and VR-ACS7 transcript levels, while it effectively abolished both the IAA- and kinetin-induced accumulation of VR-ACS1 mRNA.
The VR-ACS7 gene was isolated, and chimeric fusion between the 2.4 kb 5'-upstream region and the beta-glucuronidase (GUS) reporter gene was constructed and introduced into Nicotiana tabacum.
1(0,0,0,1) Details
14551734 Jayashree R, Rekha K, Venkatachalam P, Uratsu SL, Dandekar AM, Kumari Jayasree P, Kala RG, Priya P, Sushma Kumari S, Sobha S, Ashokan MP, Sethuraj MR, Thulaseedharan A: Genetic transformation and regeneration of rubber tree (Hevea brasiliensis Muell. Plant Cell Rep. 2003 Oct;22(3):201-9. Epub 2003 Jul 9.

Mature embryos were germinated and developed into plantlets on MS4 medium supplemented with 0.2 mg l (-1) gibberellic acid, 0.2 mg l (-1) kinetin (KIN) and 0.1 mg l (-1) indole-3-acetic acid.
The beta-glucuronidase gene (uidA) was used for screening and the neomycin phosphotransferase gene (nptII) was used for selection of the transformed calli.
1(0,0,0,1) Details
15815929 Saini R, Jaiwal PK: Transformation of a recalcitrant grain legume, Vigna mungo L. Plant Cell Rep. 2005 Jun;24(3):164-71. Epub 2005 Apr 7.


Hepper transformation was significantly increased from an average of 1% to 6.5% by using shoot apices excised from embryonic axes precultured on 10 microM benzyl-6-aminopurine (BAP) for 3 days and wounded prior to inoculation in Agrobacterium tumefaciens strain EHA105 carrying the binary vector pCAMBIA2301, which contains a neomycin phosphotransferase gene (nptII) and a beta-glucuronidase (GUS) gene (gusA) interrupted by an intron.
1(0,0,0,1) Details
19655147 Wu AM, Hu JS, Liu JY: Functional analysis of a cotton cellulose synthase A4 gene promoter in transgenic tobacco plants. Plant Cell Rep. 2009 Oct;28(10):1539-48. Epub 2009 Aug 5.

A 1,482-bp promoter sequence of the cotton cellulose synthase gene (GhCesA4) was isolated from Chinese cultivar CRI12 of Gossypium hirsutum, and transcriptionally fused to a beta-glucuronidase (GUS) reporter gene for investigation of important regions controlling gene expression in transgenic tobacco plants.
Exposure of the transgenic tobacco to various abiotic stresses showed that the full-length construct predominantly responded to NAA, kinetin, and sugar.
1(0,0,0,1) Details
7697294 Yabe N, Takahashi T, Komeda Y: Analysis of tissue-specific expression of Arabidopsis thaliana HSP90-family gene HSP81. Plant Cell Physiol. 1994 Dec;35(8):1207-19.

In situ hybridization and histochemical analysis using transgenic plants carrying chimeric gene fusions, with an HSP81 promoter region fused to a beta-glucuronidase (GUS) gene, confirmed these results.
Exogenous application of various chemicals such as ABA, GA3, kinetin, IAA, NaCl, and mannitol revealed that 10 mM IAA and 0.1 M NaCl significantly enhanced the accumulation of HSP81-2 and -3 transcripts.
1(0,0,0,1) Details
18057938 Facchini PJ, Loukanina N, Blanche V: Genetic transformation via somatic embryogenesis to establish herbicide-resistant opium poppy. Plant Cell Rep. 2008 Apr;27(4):719-27. Epub 2007 Dec 5.


Transformation was mediated by Agrobacterium tumefaciens using the pCAMBIA3301 vector, which harbors the phosphinothricin acetyltransferase (pat) gene driven by a tandem repeat of the cauliflower mosaic virus (CaMV) 35S promoter and the beta-glucuronidase (gus) structural gene driven by a single copy of the CaMV 35S promoter between left- and right-border sequences.
1(0,0,0,1) Details
8453300 Uknes S, Dincher S, Friedrich L, Negrotto D, Williams S, Thompson-Taylor H, Potter S, Ward E, Ryals J: Regulation of pathogenesis-related protein-1a gene expression in tobacco. Plant Cell. 1993 Feb;5(2):159-69.


Several artifacts, most importantly ectopic expression in pollen, were associated with the use of the beta-glucuronidase reporter gene.
1(0,0,0,1) Details
18335952 O'Neill KM, Larsen JS, Curtis WR: Scale-up of Agrobacterium-mediated transient protein expression in bioreactor-grown Nicotiana glutinosa plant cell suspension culture. Biotechnol Prog. 2008 Mar-Apr;24(2):372-6. Epub 2008 Mar 12.


The reporter gene beta-glucuronidase was transiently expressed in a 51-L bioreactor-grown plant cell suspension culture of Nicotiana glutinosa at a yield of approximately 1.1 mg through co-culture with an auxotrophic strain of Agrobacterium tumefaciens.
1(0,0,0,1) Details