Protein Information

Name glyceraldehyde 3 phosphate dehydrogenase
Synonyms CDABP0047; G3PD; GAPD; GAPD protein; GAPDH; Glyceraldehyde 3 phosphate dehydrogenase; GAPD proteins; Glyceraldehyde 3 phosphate dehydrogenases

Compound Information

Name formaldehyde
CAS formaldehyde

Reference List

PubMed Abstract RScore(About this table)
19202676 Tallima H, El Ridi R: Schistosoma mansoni glyceraldehyde 3-phosphate dehydrogenase is a lung-stage schistosomula surface membrane antigen. Folia Parasitol. 2008 Sep;55(3):180-6.

We have previously reported that Schistosoma mansoni larvae emerging from host lung at pH 7.5-7.8 and then fixed with diluted formaldehyde (HCHO) readily bind radiation-attenuated cercariae (RA) vaccine serum antibodies, as assessed by indirect membrane immunofluorescence (IF).
2(0,0,0,2) Details
19425073 Kaneko T, Okiji T, Kaneko R, Suda H, Nor JE: Gene expression analysis of immunostained endothelial cells isolated from formaldehyde-fixated paraffin embedded tumors using laser capture microdissection-A technical report. Microsc Res Tech. 2009 May 7;72(12):908-912.

Glyceraldehyde-3-phosphate dehydrogenase and 18S were used as house keeping genes for RT-PCR and real time PCR, respectively.
1(0,0,0,1) Details
17690213 Wang F, Wang L, Briggs C, Sicinska E, Gaston SM, Mamon H, Kulke MH, Zamponi R, Loda M, Maher E, Ogino S, Fuchs CS, Li J, Hader C, Makrigiorgos GM: DNA degradation test predicts success in whole-genome amplification from diverse clinical samples. J Mol Diagn. 2007 Sep;9(4):441-51. Epub 2007 Aug 9.


A multiplex PCR amplification of four glyceraldehyde-3-phosphate dehydrogenase amplicons of varying sizes was performed using genomic DNA from clinical samples, followed by size discrimination on agarose gel or fluorescent denaturing high-performance liquid chromatography (dHPLC).
1(0,0,0,1) Details
17559573 Reher M, Gebhard S, Schonheit P: Glyceraldehyde-3-phosphate ferredoxin oxidoreductase (GAPOR) and nonphosphorylating glyceraldehyde-3-phosphate dehydrogenase (GAPN), key enzymes of the respective modified Embden-Meyerhof pathways in the hyperthermophilic crenarchaeota Pyrobaculum aerophilum and Aeropyrum pernix. FEMS Microbiol Lett. 2007 Aug;273(2):196-205. Epub 2007 Jun 9.

The enzyme showed high specificity for glyceraldehyde-3-phosphate and did not use glyceraldehyde, acetaldehyde or formaldehyde as substrates.
1(0,0,0,1) Details
17981405 Witchell J, Varshney D, Gajjar T, Wangoo A, Goyal M: RNA isolation and quantitative PCR from HOPE- and formalin-fixed bovine lymph node tissues. Pathol Res Pract. 2008;204(2):105-11. Epub 2007 Nov 5.


Following qRT-PCR for glyceraldehyde-3-phosphate dehydrogenase (GAPDH), total RNA from HOPE-fixed tissues showed higher levels of target messenger ribonucleic acid (mRNA) (4.05 x 10 (-2) pg/100 ng total RNA using the commercial kit and 6.45 x 10 (-2) pg/100 ng total RNA using Trizol) in comparison to formalin-fixed tissues (5.69 x 10 (-4) pg/100 ng total RNA).
1(0,0,0,1) Details
18410440 Rogerson L, Darby S, Jabbar T, Mathers ME, Leung HY, Robson CN, Sahadevan K, O'Toole K, Gnanapragasam VJ: Application of transcript profiling in formalin-fixed paraffin-embedded diagnostic prostate cancer needle biopsies. BJU Int. 2008 Aug;102(3):364-70. Epub 2008 Apr 11.


RESULTS: LCM and RNA extraction was achieved in all biopsies from a pilot cohort of five patients. cDNA produced was successfully used to detect expression of glyceraldehyde-3-phosphate dehydrogenase, RPL13, prostate-specific antigen, vimentin, inhibitor of differentiation/DNA binding 1 (Id-1) and polycomb group protein enhancer of zeste homolog 2 (EZH2) transcripts.
1(0,0,0,1) Details