| Name | 26S proteasome (protein family or complex) |
|---|---|
| Synonyms | 26 proteasome; 26S Proteasome; 26S protease; 26S proteases |
| Name | cycloheximide |
|---|---|
| CAS |
| PubMed | Abstract | RScore(About this table) | |
|---|---|---|---|
| 16243960 | Korashy HM, El-Kadi AO: Transcriptional regulation of the NAD (P) H:quinone oxidoreductase 1 and glutathione S-transferase ya genes by mercury, lead, and Drug Metab Dispos. 2006 Jan;34(1):152-65. Epub 2005 Oct 21. Furthermore, inhibition of Nrf2 protein degradation by carbobenzoxy-L-leucyl-L-leucyl-leucinal (MG-132), a 26S proteasome inhibitor, significantly reversed the cycloheximide-mediated inhibition of Nqo1 and Gst ya mRNAs, which coincided with an increase in the expression of Nrf2, confirming that a transcriptional mechanism is involved. |
31(0,1,1,1) | Details |
| 17606337 | Elbekai RH, El-Kadi AO: Transcriptional activation and posttranscriptional modification of Cyp1a1 by arsenite, cadmium, and Toxicol Lett. 2007 Aug;172(3):106-19. Epub 2007 May 24. The metal-mediated induction of Cyp1a1 mRNA was further potentiated by the protein synthesis inhibitor, cycloheximide and the 26S proteasome inhibitor, MG-132, but completely inhibited by the RNA transcription inhibitor, actinomycin-D, implying a transcriptional regulation of the Cyp1a1 gene by the heavy metals. |
31(0,1,1,1) | Details |
| 20087596 | Kurepa J, Karangwa C, Duke LS, Smalle JA: Arabidopsis sensitivity to protein synthesis inhibitors depends on 26S proteasome activity. Plant Cell Rep. 2010 Mar;29(3):249-59. Epub 2010 Jan 20. We show that the rpt2a-3, rpn10-1 and rpn12a-1 mutants are hypersensitive to the antibiotic hygromycin B, and tolerant to the translation inhibitor cycloheximide (CHX) and herbicide L-phosphinothricin (PPT). |
2(0,0,0,2) | Details |
| 16206267 | Lovborg H, Oberg F, Rickardson L, Gullbo J, Nygren P, Larsson R: Inhibition of proteasome activity, nuclear factor-KappaB translocation and cell survival by the antialcoholism drug disulfiram. Int J Cancer. 2006 Mar 15;118(6):1577-80. Here, we identify the antialcoholism drug disulfiram and its analogue pyrrolidine dithiocarbamate (PDTC) as inhibitors of the 26S proteasome activity in a cell-based screening assay. |
2(0,0,0,2) | Details |
| 16359394 | Shen H, Moon J, Huq E: PIF1 is regulated by light-mediated degradation through the ubiquitin-26S proteasome pathway to optimize photomorphogenesis of seedlings in Arabidopsis. Plant J. 2005 Dec;44(6):1023-35. Further, de novo protein synthesis is not required for degradation of PIF1, as the presence of cycloheximide does not prevent degradation of PIF1 in the light. |
2(0,0,0,2) | Details |
| 18077523 | Hsieh CH, Chen FD, Wang HE, Hwang JJ, Chang CW, Lee YJ, Gelovani JG, Liu RS: Generation of destabilized herpes simplex virus type 1 thymidine kinase as transcription reporter for PET reporter systems in molecular genetic imaging. J Nucl Med. 2008 Jan;49(1):142-50. Epub 2007 Dec 12. RESULTS: The destabilized HSV1-TK, unlike wild-type HSV1-TK, was unstable in the presence of cycloheximide and had a short half-life of protein and enzyme activity. The proteasome inhibition assay was used to test whether the rapid turnover of the destabilized HSV1-TK was processed in a 26S proteasome-dependent manner. |
2(0,0,0,2) | Details |
| 18273947 | Staszczak M: The role of the ubiquitin-proteasome system in the response of the ligninolytic fungus Trametes versicolor to deprivation. Fungal Genet Biol. 2008 Mar;45(3):328-37. Furthermore, it was found that deprivation affected 26S proteasome activities of T. versicolor. |
1(0,0,0,1) | Details |
| 18339854 | Shekhar MP, Gerard B, Pauley RJ, Williams BO, Tait L: Rad6B is a positive regulator of beta-catenin stabilization. Cancer Res. 2008 Mar 15;68(6):1741-50. Measurement of beta-catenin protein stability by cycloheximide treatment showed that Rad6B silencing specifically decreases the stability of high molecular beta-catenin with minimal effect upon the 90-kDa nascent form. In vitro ubiquitination assays confirmed that Rad6B mediates beta-catenin polyubiquitination, and ubiquitin chain extensions involve 63 residues that are insensitive to 26S proteasome. |
1(0,0,0,1) | Details |
| 19910419 | Hsieh CH, Kuo JW, Lee YJ, Chang CW, Gelovani JG, Liu RS: Construction of mutant TKGFP for real-time imaging of temporal dynamics of HIF-1 signal transduction activity mediated by hypoxia and reoxygenation in tumors in living mice. J Nucl Med. 2009 Dec;50(12):2049-57. Epub 2009 Nov 12. Rapid turnover of NESTKGFP:dMODC occurred in a 26S proteasome-dependent manner. RESULTS: Unlike TKGFP, NESTKGFP:dMODC was unstable in the presence of cycloheximide and showed a short half-life of protein and enzyme activity. |
1(0,0,0,1) | Details |
| 19954423 | Azzu V, Mookerjee SA, Brand MD: Rapid turnover of mitochondrial uncoupling protein 3. . Biochem J. 2010 Jan 27;426(1):13-7. UCP2 is known to have a short half-life of approx. 1 h, owing to its rapid degradation by the cytosolic 26S proteasome, whereas UCP1 is turned over much more slowly by mitochondrial autophagy. UCP3 half-life was examined in the mouse C2C12 myoblast cell line by inhibiting protein synthesis with cycloheximide and monitoring UCP3 protein levels by immunoblot analysis. |
1(0,0,0,1) | Details |
| 17400707 | Qiu QS, Hardin SC, Mace J, Brutnell TP, Huber SC: Light and metabolic signals control the selective degradation of synthase in maize leaves during deetiolation. Plant Physiol. 2007 May;144(1):468-78. Epub 2007 Mar 30. However, SUS degradation was strongly inhibited by feeding cycloheximide or amino acids to detached leaves, while Suc feeding had no effect. |
0(0,0,0,0) | Details |
| 18655183 | Lee DH, Lee YJ: suppresses hypoxia-induced accumulation of hypoxia-inducible factor-1alpha (HIF-1alpha) through inhibiting protein synthesis. J Cell Biochem. 2008 Oct 1;105(2):546-53. Treatment with 100 microM cycloheximide, a protein synthesis inhibitor, replicated the effect of by inhibiting HIF-1alpha accumulation during hypoxia. |
0(0,0,0,0) | Details |
| 16093525 | Korashy HM, El-Kadi AO: Regulatory mechanisms modulating the expression of cytochrome P450 1A1 gene by heavy metals. Toxicol Sci. 2005 Nov;88(1):39-51. Epub 2005 Aug 10. The protein synthesis inhibitor, cycloheximide, and 26S proteasome inhibitor, carbobenzoxy-L-leucyl-L-leucyl-leucinal (MG-132), super-induced the metal-mediated induction of Cyp1a1 mRNA. |
31(0,1,1,1) | Details |