| Name | lactate dehydrogenase (protein family or complex) |
|---|---|
| Synonyms | LDH; lactate dehydrogenase; lactate dehydrogenases |
| Name | cycloheximide |
|---|---|
| CAS |
| PubMed | Abstract | RScore(About this table) | |
|---|---|---|---|
| 16956375 | Leak RK, Liou AK, Zigmond MJ: Effect of sublethal on the response to subsequent oxidative stress in dopaminergic cells: evidence for preconditioning. J Neurochem. 2006 Nov;99(4):1151-63. Epub 2006 Sep 4. The antioxidant, N-acetyl (20 mM), when applied during preconditioning, abolished protection, as did the protein synthesis inhibitor, cycloheximide (0.2 microM). |
0(0,0,0,0) | Details |
| 19559790 | Shioiri T, Muroi M, Hatao F, Nishida M, Ogawa T, Mimura Y, Seto Y, Kaminishi M, Tanamoto K: Caspase-3 is activated and rapidly released from human umbilical vein endothelial cells in response to lipopolysaccharide. Biochim Biophys Acta. 2009 Oct;1792(10):1011-8. Epub 2009 Jun 25. In the presence of cycloheximide (CHX), an increase in intracellular caspase-3/7 activity in response to LPS was not detected in HUVEC up to 24 h following stimulation even in the presence of LPS-binding protein (LBP), soluble CD14 and soluble MD-2, whereas the decrease in cell viability and increase in release of the cellular enzyme lactate dehydrogenase (LDH) were observed in a soluble CD14/LBP-dependent manner. |
0(0,0,0,0) | Details |
| 16034370 | Ma D, Hossain M, Pettet GK, Luo Y, Lim T, Akimov S, Sanders RD, Franks NP, Maze M: Xenon preconditioning reduces brain damage from neonatal asphyxia in rats. J Cereb Blood Flow Metab. 2006 Feb;26(2):199-208. In a neuronal-glial cell coculture, preexposure to xenon for 2 h caused a concentration-dependent reduction of lactate dehydrogenase release from cells deprived of and 24 h later; xenon's preconditioning effect was abolished by cycloheximide, a protein synthesis inhibitor. |
31(0,1,1,1) | Details |
| 19002967 | Haslam G, Wyatt D, Kitos PA: Estimating the number of viable animal cells in multi-well cultures based on their lactate dehydrogenase activities. Cytotechnology. 2000 Jan;32(1):63-75. |
2(0,0,0,2) | Details |
| 19454725 | Karahashi H, Michelsen KS, Arditi M: Lipopolysaccharide-induced apoptosis in transformed bovine brain endothelial cells and human dermal microvessel endothelial cells: the role of JNK. J Immunol. 2009 Jun 1;182(11):7280-6. Stimulation of transformed bovine brain endothelial cells (TBBEC) with LPS leads to apoptosis while human microvessel endothelial cells (HMEC) need the presence of cycloheximide (CHX) with LPS to induce apoptosis. LPS-induced apoptosis in TBBEC was hallmarked by the activation of caspase 3, caspase 6, and caspase 8 after the stimulation of LPS, followed by poly (ADP- polymerase cleavage and lactate dehydrogenase release. |
2(0,0,0,2) | Details |
| 19583809 | Keating N, Mroz MS, Scharl MM, Marsh C, Ferguson G, Hofmann AF, Keely SJ: Physiological concentrations of bile acids down-regulate agonist induced secretion in colonic epithelial cells. J Cell Mol Med. 2009 Aug;13(8B):2293-303. Epub 2009 Jul 6. The EGFr inhibitor, AG1478, and the protein synthesis inhibitor, cycloheximide, reversed the antisecretory effects of DCA, while the MAPK inhibitors, PD98059 and SB203580, did not. At high concentrations (0.5-1 mM), DCA acutely stimulated Cl (-) secretion but this effect was associated with cell injury, as evidenced by decreased transepithelial resistance (TER) and increased lactate dehydrogenase (LDH) release. |
1(0,0,0,1) | Details |
| 15935204 | Dreiem A, Ring A, Fonnum F: Organic solvent-induced cell death in rat cerebellar granule cells: structure dependence of c10 hydrocarbons and relationship to reactive species formation. Neurotoxicology. 2005 Jun;26(3):321-30. Cell death was assessed by measurement of lactate dehydrogenase (LDH) release and trypan blue staining. t-butylcyclohexane and n-butylbenzene induced cell death in rat CGCs in a time-dependent and concentration-dependent manner. However, there was no internucleosomal DNA fragmentation and no protection by the pan-caspase inhibitor Boc-D-FMK or the protein synthesis inhibitor cycloheximide. |
1(0,0,0,1) | Details |
| 16192422 | Yung S, Tsang RC, Sun Y, Leung JK, Chan TM: Effect of human anti-DNA antibodies on proximal renal tubular epithelial cell cytokine expression: implications on tubulointerstitial inflammation in lupus nephritis. J Am Soc Nephrol. 2005 Nov;16(11):3281-94. Epub 2005 Sep 28. This was accompanied by altered cell morphology, increased cell proliferation (P < 0.05), and lactate dehydrogenase release (P < 0.05). |
1(0,0,0,1) | Details |
| 19453217 | Vinken M, Decrock E, De Vuyst E, Leybaert L, Vanhaecke T, Rogiers V: Biochemical characterisation of an in vitro model of hepatocellular apoptotic cell death. Altern Lab Anim. 2009 Apr;37(2):209-18. This study was set up to critically evaluate a commonly-used in vitro model of hepatocellular apoptotic cell death, in which freshly isolated hepatocytes, cultured in a monolayer configuration, are exposed to a combination of Fas ligand and cycloheximide for six hours. A set of well-acknowledged cell death markers was addressed: a) cell morphology was studied by light microscopy; b) apoptotic and necrotic cell populations were quantified by in situ staining with Annexin-V, Hoechst 33342 and propidium iodide (PI); c) apoptotic and necrotic activities were monitored by probing caspase 3-like activity and measuring the extracellular leakage of lactate dehydrogenase (LDH), respectively; and d) the expression of apoptosis regulators was investigated by immunoblotting. |
1(0,0,0,1) | Details |
| 18672029 | Jang HJ, Hwang S, Cho KY, Kim do K, Chay KO, Kim JK: Taxol induces oxidative neuronal cell death by enhancing the activity of oxidase in mouse cortical cultures. Neurosci Lett. 2008 Sep 26;443(1):17-22. Epub 2008 Jul 23. Cell death was assessed by measuring lactate dehydrogenase in the bathing media after 24-h exposure to taxol. The neuronal death induced by taxol was significantly attenuated not only by anti-apoptotic drugs such as z-VAD-fmk and cycloheximide but also by antioxidants such as trolox, and tempol. |
1(0,0,0,1) | Details |