Protein Information

Name CD4
Synonyms CD4; CD4 antigen (p55); CD4 molecule; CD4mut; T cell antigen T4/LEU3; T cell surface glycoprotein CD4; T cell surface antigen T4/Leu 3; T cell surface glycoprotein CD4 precursor…

Compound Information

Name cycloheximide
CAS

Reference List

PubMed Abstract RScore(About this table)
16237056 Park IK, Shultz LD, Letterio JJ, Gorham JD: TGF-beta1 inhibits T-bet induction by IFN-gamma in murine CD4+ T cells through the protein tyrosine phosphatase Src homology region 2 domain-containing phosphatase-1. J Immunol. 2005 Nov 1;175(9):5666-74.

4(0,0,0,4) Details
16425129 Sasson SC, Zaunders JJ, Zanetti G, King EM, Merlin KM, Smith DE, Stanley KK, Cooper DA, Kelleher AD: Increased plasma interleukin-7 level correlates with decreased CD127 and Increased CD132 extracellular expression on T cell subsets in patients with HIV-1 infection. J Infect Dis. 2006 Feb 15;193(4):505-14. Epub 2006 Jan 17.

The effects of IL-7 and cycloheximide on IL-7R expression by peripheral blood mononuclear cells were examined in vitro.
Plasma IL-7 levels positively correlated with CD4 (+) T cell immune reconstitution in patients with PHI.
3(0,0,0,3) Details
17182689 Mangino G, Percario ZA, Fiorucci G, Vaccari G, Manrique S, Romeo G, Federico M, Geyer M, Affabris E: In vitro treatment of human monocytes/macrophages with myristoylated recombinant Nef of human immunodeficiency virus type 1 leads to the activation of mitogen-activated protein kinases, IkappaB kinases, and interferon regulatory factor 3 and to the release of beta interferon. J Virol. 2007 Mar;81(6):2777-91. Epub 2006 Dec 20.

Nef regulates the cell surface expression of critical proteins (including down-regulation of CD4 and major histocompatibility complex class I), T-cell receptor signaling, and apoptosis, inducing proapoptotic effects in uninfected bystander cells and antiapoptotic effects in infected cells.
Previously, we reported that Nef treatment of primary human monocyte-derived macrophages (MDMs) induces a cycloheximide-independent activation of NF-kappaB and the synthesis and secretion of a set of chemokines/cytokines that activate STAT1 and STAT3.
1(0,0,0,1) Details
18718855 Kondo K, Okuma K, Tanaka R, Matsuzaki G, Ansari AA, Tanaka Y: Rapid induction of OX40 ligand on primary T cells activated under DNA-damaging conditions. Hum Immunol. 2008 Sep;69(9):533-42. Epub 2008 Aug 15.

Flow cytometric analyses demonstrated that a significant frequency of these DNA-damaged activated primary CD4+ and CD8+ T cells became OX40L+ as early as 1 hour after treatment.
The OX40L induction on the DNA-damaged activated T cells was inhibited by treatment with either RNA or protein synthesis inhibitors, actinomycin D, or cycloheximide, respectively.
1(0,0,0,1) Details
17164472 Heo Y, Mondal TK, Gao D, Kasten-Jolly J, Kishikawa H, Lawrence DA: Posttranscriptional inhibition of interferon-gamma production by lead. Toxicol Sci. 2007 Mar;96(1):92-100. Epub 2006 Dec 12.

Lead (Pb) is known to preferentially suppress the activation and development of type-1 CD4+ helper T cell (Th1) responses, whereas it enhances the development of type-2 CD4+ helper T cell (Th2) responses.
Pb did not enhance protein degradation of IFNgamma, in that lactacystin, an effective blocker of proteosomal proteolysis, did not prevent loss of IFNgamma; additionally, Pb did not accelerate loss of IFNgamma after cycloheximide treatment.
1(0,0,0,1) Details
19665696 Oguma T, Ono T, Kajiwara T, Sato M, Miyahira Y, Arino H, Yoshihara Y, Tadakuma T: CD4 (+) CD8 (+) thymocytes are induced to cell death by a small dose of puromycin via ER stress. Cell Immunol. 2009;260(1):21-7. Epub 2009 Jul 14.

The induction of apoptosis was blocked by the protein synthesis inhibitor cycloheximide, and to some extent by transfection of Bcl-xL or Bcl-2 genes.
3(0,0,0,3) Details
16959949 Verma-Gandhu M, Verdu EF, Cohen-Lyons D, Collins SM: Lymphocyte-mediated regulation of beta-endorphin in the myenteric plexus. Am J Physiol Gastrointest Liver Physiol. 2007 Jan;292(1):G344-8. Epub 2006 Sep 7.

Previously, we have shown that adoptive transfer of CD4+ T cells to severe combined immune-deficient (SCID) mice normalized immunodeficiency-related visceral hyperalgesia.
However, exogenous beta-endorphin did upregulate beta-endorphin expression, and both cycloheximide and naloxone methiodide inhibited peptide upregulation.
2(0,0,0,2) Details
18337562 Murooka TT, Rahbar R, Platanias LC, Fish EN: CCL5-mediated T-cell chemotaxis involves the initiation of mRNA translation through mTOR/4E-BP1. Blood. 2008 May 15;111(10):4892-901. Epub 2008 Mar 12.

Here, we examined the role for CCL5-mediated initiation of mRNA translation in CD4 (+) T-cell chemotaxis.
Cycloheximide, but not actinomycin D, significantly reduced chemotaxis, suggesting a possible role for mRNA translation in T-cell migration.
2(0,0,0,2) Details
19447459 Mikulak J, Gianolini M, Versmisse P, Pancino G, Lusso P, Verani A: Biological and physical characterization of the X4 HIV-1 suppressive factor secreted by LPS-stimulated human macrophages. Virology. 2009 Jul 20;390(1):37-44. Epub 2009 May 17.

LPS-stimulated macrophages release soluble factors that inhibit HIV-1 infection in both CD4 (+) T lymphocytes and macrophages.
MDAF is sensitive to heat and proteinase K treatment, and it appears to be preformed within MDM, in that it is rapidly released upon LPS stimulation and its production is insensitive to cycloheximide, an inhibitor of protein neosynthesis.
1(0,0,0,1) Details
16751404 Kubota K: A novel functional T cell hybridoma recognizes macrophage cell death induced by bacteria: a possible role for innate lymphocytes in bacterial infection. J Immunol. 2006 Jun 15;176(12):7576-88.

We have established a novel TCRalphabeta (TCRVbeta6)(+) CD4 (-) CD8 (-) T cell hybridoma designated B6HO3.
Cycloheximide and Abs to H-2K (d), H-2D (d), Ia (d), CD1d, TCRVbeta6, and IL-12 did not inhibit the contact-dependent IFN-gamma response, indicating that this IFN-gamma response did not require de novo protein synthesis in bacterial-infected J774 cells and was TCR and IL-12 independent.
1(0,0,0,1) Details
16777169 Choudhary SK, Walker RM, Powell DM, Planelles V, Walsh C, Camerini D: CXCR4 tropic human immunodeficiency virus type 1 induces an apoptotic cascade in immature infected thymocytes that resembles thymocyte negative selection. Virology. 2006 Sep 1;352(2):268-84. Epub 2006 Jun 14.

Moreover, X4-HIV-1 induced apoptosis primarily in immature CD4+ CD8+ (DP) thymocytes whereas most mature CD4 or CD8 single-positive (SP) thymocytes were resistant to X4 HIV-1-induced apoptosis despite infection.
Furthermore, treatment of thymocytes with cycloheximide abrogated HIV-1-induced apoptosis, implying a requirement for de novo protein synthesis.
1(0,0,0,1) Details