| Name | interleukin (protein family or complex) |
|---|---|
| Synonyms | Interleukin; Interleukins; interleukin receptor; interleukin receptors |
| Name | cycloheximide |
|---|---|
| CAS |
| PubMed | Abstract | RScore(About this table) | |
|---|---|---|---|
| 17178924 | Moreau ME, Bawolak MT, Morissette G, Adam A, Marceau F: Role of nuclear factor-kappaB and protein kinase C signaling in the expression of the kinin B1 receptor in human vascular smooth muscle cells. Mol Pharmacol. 2007 Mar;71(3):949-56. Epub 2006 Dec 18. Various stimulatory compounds that increase receptor mRNA stability only (human and bovine sera, cycloheximide) or that stimulate NF-kappaB nuclear translocation and both mRNA concentration and stability [interleukin (IL)-1beta, phorbol 12- 13- (PMA)] all increased the density of binding sites for the tritiated B1 receptor agonist [3H] -des-Arg9-bradykinin (without change in receptor affinity) in cell-based assays. |
31(0,1,1,1) | Details |
| 16297161 | Hormdee D, Nagasawa T, Kiji M, Yashiro R, Kobayashi H, Koshy G, Noguchi K, Nitta H, Ishikawa I: Protein kinase-A-dependent osteoprotegerin production on interleukin-1 stimulation in human gingival fibroblasts is distinct from periodontal ligament fibroblasts. Clin Exp Immunol. 2005 Dec;142(3):490-7. Periodontitis, a chronic inflammatory disease, is characterized by increased expression of interleukin (IL)-1 and other inflammatory mediators resulting in extensive osteoclast formation and bone loss. Human gingival fibroblasts (HGF) and periodontal ligament fibroblasts (PDL) were stimulated with IL-1alpha with or without protein synthesis inhibitor cycloheximide (CHX), protein kinase A (PKA) inhibitors, protein kinase C (PKC) inhibitors and (2) (PGE (2)) inhibitor. |
1(0,0,0,1) | Details |
| 15640153 | Pillinger MH, Marjanovic N, Kim SY, Scher JU, Izmirly P, Tolani S, Dinsell V, Lee YC, Blaser MJ, Abramson SB: Matrix metalloproteinase secretion by gastric epithelial cells is regulated by E prostaglandins and MAPKs. J Biol Chem. 2005 Mar 18;280(11):9973-9. Epub 2005 Jan 7. Tumor necrosis factor (TNF)-alpha, interleukin (IL)-1beta, and epidermal growth factor (EGF) stimulated gastric cell MMP-1 secretion, indicating that MMP-1 secretion occurs in inflammatory as well as non-inflammatory situations. |
1(0,0,0,1) | Details |
| 18854426 | Gu JQ, Ikuyama S, Wei P, Fan B, Oyama J, Inoguchi T, Nishimura J: Pycnogenol, an extract from French maritime pine, suppresses Toll-like receptor 4-mediated expression of adipose differentiation-related protein in macrophages. Am J Physiol Endocrinol Metab. 2008 Dec;295(6):E1390-400. Epub 2008 Oct 14. Actinomycin D almost completely abolished the LPS effect, whereas cycloheximide decreased the expression at 12 h, indicating that the LPS-induced ADRP expression was stimulated at the transcriptional level and was also mediated by new protein synthesis. In addition, preceding the increase of the ADRP mRNA, LPS induced the expression of interleukin (IL)-6, IL-1alpha, and interferon-beta mRNAs, all of which stimulated the ADRP expression. |
1(0,0,0,1) | Details |
| 16087364 | Relova AJ, Shahana S, Makeeva N, Roomans GM: Effect of cytokines on ICAM-1 and ZO-1 expression on human airway epithelial cells. Cell Biol Int. 2005 Sep;29(9):768-77. The study aimed at investigating the effects of interleukin (IL)-4, IL-8, IL-13 and interferon-gamma (IFN-gamma) on cell viability and intracellular adhesion molecule (ICAM)-1 and zonula occludens protein (ZO)-1 expression on cultured human basal and columnar airway epithelial cells. Cycloheximide (CHX) induced cell death in both cell lines. |
1(0,0,0,1) | Details |
| 17641275 | Kwakkel J, Wiersinga WM, Boelen A: Interleukin-1beta modulates endogenous thyroid hormone receptor alpha gene transcription in liver cells. J Endocrinol. 2007 Aug;194(2):257-65. Cycloheximide experiments showed that the decreased promoter activity is independent of de novo protein synthesis and therefore most likely due to posttranslational modifications such as phosphorylation or subcellular relocalization. The proinflammatory cytokine interleukin (IL)-1beta decreases thyroid hormone receptor (TR) beta1 mRNA in an NFkappaB-dependent way. |
1(0,0,0,1) | Details |
| 19578789 | Kamimoto M, Mizuno S, Nakamura T: Reciprocal regulation of IL-6 and IL-10 balance by HGF via recruitment of heme oxygenase-1 in macrophages for attenuation of liver injury in a mouse model of endotoxemia. Int J Mol Med. 2009 Aug;24(2):161-70. In the culture of macrophages (Raw264.7), HGF enhanced the LPS-mediated HO-1 induction, and this effect was abolished by cycloheximide, but not by actinomycin-D, thus suggesting that a post-transcriptional pathway is involved in HGF-mediated up-regulation of HO-1. In this process, interleukin (IL)-6 and IL-10 are key contributors for eliciting pro- and anti-inflammatory responses, respectively. |
1(0,0,0,1) | Details |
| 18005266 | Ratthe C, Girard D: Investigation of the interleukin (IL)-4/IL-4 receptor system in promyelocytic leukaemia PLB-985 cells during differentiation toward neutrophil-like phenotype: mechanism involved in IL-4-induced SOCS3 protein expression. Br J Haematol. 2008 Jan;140(1):59-70. Epub 2007 Nov 12. Utilization of catalase, cycloheximide and inhibitors showed that IL-4 induced SOCS3 by a mechanism dependent on a complete oxidase complex, protein synthesis and phosphorylation, but independent of production of reactive species. |
1(0,0,0,1) | Details |
| 20054003 | McLaughlin M, Alloza I, Quoc HP, Scott CJ, Hirabayashi Y, Vandenbroeck K: Inhibition of secretion of interleukin (IL)-12/IL-23 family cytokines by 4-trifluoromethyl- is coupled to degradation via the endoplasmic reticulum stress protein HERP. J Biol Chem. 2010 Mar 5;285(10):6960-9. Epub 2010 Jan 6. Both HERP siRNA knockdown and HERP overexpression coupled to cycloheximide chase assays revealed that HERP is necessary for degradation of intracellularly retained p80 by TFM-C. |
1(0,0,0,1) | Details |
| 17308068 | Bachmann M, Dragoi C, Poleganov MA, Pfeilschifter J, Muhl H: Interleukin-18 directly activates T-bet expression and function via p38 mitogen-activated protein kinase and nuclear factor-kappaB in acute myeloid leukemia-derived predendritic KG-1 cells. Mol Cancer Ther. 2007 Feb;6(2):723-31. The T helper type 1 cytokine interleukin (IL)-18 has been shown to induce the maturation of these cells towards a dendritic phenotype and, moreover, is able to mediate IFNgamma production in this model. Blockage of translation by cycloheximide, usage of neutralizing antibodies, and the inability of IFNgamma to mediate significant p38 mitogen-activated protein kinase activation in KG-1 cells clearly revealed that activation of T-bet was not via autocrine IFNgamma. |
1(0,0,0,1) | Details |
| 16425129 | Sasson SC, Zaunders JJ, Zanetti G, King EM, Merlin KM, Smith DE, Stanley KK, Cooper DA, Kelleher AD: Increased plasma interleukin-7 level correlates with decreased CD127 and Increased CD132 extracellular expression on T cell subsets in patients with HIV-1 infection. J Infect Dis. 2006 Feb 15;193(4):505-14. Epub 2006 Jan 17. BACKGROUND: Interleukin (IL)-7 levels are increased in patients with human immunodeficiency virus type 1 (HIV-1)-associated lymphopenia; however, the effects of this on IL-7 receptor (IL-7R) expression, disease progression, and immune reconstitution remain unclear. The effects of IL-7 and cycloheximide on IL-7R expression by peripheral blood mononuclear cells were examined in vitro. |
1(0,0,0,1) | Details |
| 18729741 | Shizu M, Itoh Y, Sunahara R, Chujo S, Hayashi H, Ide Y, Takii T, Koshiko M, Chung SW, Hayakawa K, Miyazawa K, Hirose K, Onozaki K: Cigarette smoke condensate upregulates the gene and protein expression of proinflammatory cytokines in human fibroblast-like synoviocyte line. J Interferon Cytokine Res. 2008 Aug;28(8):509-21. When human fibroblast-like synoviocytes line MH7A was treated with cigarette smoke condensate (CSC), either mainstream or sidestream, expression levels of interleukin (IL)-1alpha, IL-1beta, IL-6, IL-8, and CYP1A1 mRNA were upregulated in both time- and dose-dependent manners. Cycloheximide treatment indicated that the augmenting effect of CSC on IL-1alpha, IL-1beta and IL-8, but not IL-6 and CYP1A1, mRNA expression requires de novo protein synthesis. |
1(0,0,0,1) | Details |
| 16541418 | Lu S, Dong Z: Characterization of TGF-beta-regulated interleukin-8 expression in human prostate cancer cells. Prostate. 2006 Jun 15;66(9):996-1004. BACKGROUND: Interleukin (IL)-8 and transforming growth factor (TGF)-beta1 are overexpressed in advanced prostate cancer. TGF-beta1 stimulated IL-8 expression in dose- and time-dependent manners, which was blocked by cycloheximide (CHX) and actinomycin D (ActD). |
1(0,0,0,1) | Details |
| 16264039 | Tsuji K, Yamamoto S, Ou W, Nishi N, Kobayashi I, Zaitsu M, Muro E, Sadakane Y, Ichimaru T, Hamasaki Y: dsRNA enhances eotaxin-3 production through interleukin-4 receptor upregulation in airway epithelial cells. Eur Respir J. 2005 Nov;26(5):795-803. Poly (IC)-induced upregulation of IL-4Ralpha was inhibited by treatment with cycloheximide or dexamethasone. However, the transfection of poly (IC) prior to interleukin (IL)-4 stimulation enhanced eotaxin-3 production. |
1(0,0,0,1) | Details |
| 17286758 | Endoh I, Di Girolamo N, Hampartzoumian T, Cameron B, Geczy CL, Tedla N: Ultraviolet B irradiation selectively increases the production of interleukin-8 in human cord blood-derived mast cells. Clin Exp Immunol. 2007 Apr;148(1):161-7. UVB exposure significantly increased the release of interleukin (IL)-8 and modestly increased IL-1alpha production, but cytokines such as IL-2, IL-4, IL-6, IL-10, IL-12, IL-13, tumour necrosis factor (TNF)-alpha and interferon (IFN)-gamma were unaffected. Cycloheximide reduced the UVB-mediated induction of IL-8 by 30-40%, suggesting that new protein synthesis contributed to IL-8 production. |
1(0,0,0,1) | Details |
| 15722640 | Park CS, Choi EN, Kim JS, Choi YS, Rhim TY, Chang HS, Chung IY: Interferon-gamma inhibits in vitro mobilization of eosinophils by interleukin-5. Int Arch Allergy Immunol. 2005 Mar;136(3):295-302. Epub 2005 Feb 16. RESULTS: Interleukin (IL)-5, IL-6, and tumor necrosis factor (TNF) induced an eosinophil shape change, whereas interferon (IFN)-gamma significantly inhibited the shape change. We further analyzed the IFN-gamma effect, showing that pretreatment with IFN-gamma strongly suppressed IL-5-induced eosinophil shape change, and cycloheximide (CHX) abrogated the suppression by IFN-gamma, suggesting that new protein synthesis is required for the inhibitory effect by this cytokine. |
1(0,0,0,1) | Details |
| 16987239 | Ito H, Yamamoto N, Arima H, Hirate H, Morishima T, Umenishi F, Tada T, Asai K, Katsuya H, Sobue K: Interleukin-1beta induces the expression of aquaporin-4 through a nuclear factor-kappaB pathway in rat astrocytes. J Neurochem. 2006 Oct;99(1):107-18. Interleukin (IL)-1beta is known to play a role in the formation of brain edema after various types of injury. It appeared that IL-1beta increased the level of AQP4 mRNA without involvement of de novo protein synthesis because cycloheximide, a protein synthesis inhibitor, did not inhibit the effects of IL-1beta. |
1(0,0,0,1) | Details |
| 17304101 | Pearl-Yafe M, Fabian I, Halperin D, Flatau E, Werber S, Shalit I: Interferon-gamma and bacterial lipopolysaccharide act synergistically on human neutrophils enhancing interleukin-8, interleukin-1beta, tumor necrosis factor-alpha, and interleukin-12 p70 secretion and phagocytosis via upregulation of toll-like receptor 4. Shock. 2007 Mar;27(3):226-31. Using the protein synthesis inhibitor cycloheximide and measuring the expression of CD35 in neutrophils stimulated with IFN-gamma and LPS alone or in combination, we could demonstrate that IFN-gamma enhances TLR4 by de novo protein synthesis, whereas the addition of LPS acts synergistically by enhancing vesicular mobilization to the cell surface. Costimulation with IFN-gamma and LPS induced neutrophil activation and enhanced secretion of the cytokines, interleukin (IL)-8, IL-1beta, tumor necrosis factor-alpha, and IL-12 p70, and phagocytosis of latex beads, processes that were blocked by a monoclonal antibody specific for TLR4. |
1(0,0,0,1) | Details |
| 16197996 | Sato H, Miura R, Kai C: Measles virus infection induces interleukin-8 release in human pulmonary epithelial cells. Comp Immunol Microbiol Infect Dis. 2005 Jul;28(4):311-20. Epub 2005 Sep 28. In this report, we studied the effect of MV infection on the production of interleukin (IL)-8 by the pulmonary epithelial cells. The IL-8 production was little affected by UV-inactivation of MV and scarcely suppressed by cycloheximide treatment. |
1(0,0,0,1) | Details |
| 19428567 | Gu L, Dean J, Oliveira AL, Sheehy N, Hall WW, Gautier VW: Expression profile and differential regulation of the Human I-mfa domain-Containing protein (HIC) gene in immune cells. Immunol Lett. 2009 Apr 27;123(2):179-84. Epub 2009 Mar 28. To further characterise the HIC functional relationship with the immune system, we sought to analyse the HIC gene expression profile in immune cells and to determine if immunomodulatory cytokines, such as interleukin (IL)-2, could regulate the expression of HIC mRNA. Addition of cycloheximide indicated that the IL-2 effects were independent of de novo protein synthesis and that the HIC gene is a direct target of IL-2. |
1(0,0,0,1) | Details |
| 19664126 | Misiak-Tloczek A, Brzezinska-Blaszczyk E: IL-6, but not IL-4, stimulates chemokinesis and TNF stimulates chemotaxis of tissue mast cells: involvement of both mitogen-activated protein kinases and phosphatidylinositol 3-kinase signalling pathways. APMIS. 2009 Aug;117(8):558-67. Considering that interleukin (IL)-6 and IL-4, as well as tumour necrosis factor (TNF), influence mast cell activity in various ways, the purpose of the current study was to examine whether these cytokines function as rat peritoneal mast cell chemoattractants. |
1(0,0,0,1) | Details |
| 16883635 | Lee KH, Choi EM: Rubus coreanus Miq. extract promotes osteoblast differentiation and inhibits bone-resorbing mediators in MC3T3-E1 cells. Am J Chin Med. 2006;34(4):643-54. The effect of RCE (50 microg/ml) in increasing cell viability, ALP activity, and collagen content was prevented by the presence of 10 (-6) M cycloheximide and 10 (-6) M tamoxifen, suggesting that RCE's effect results from a newly synthesized protein component and might be partly involved in action. Treatment with RCE (10approximately50 microg/ml) decreased the 0.2 mM H (2) O (2)-induced apoptosis and production of tumor necrosis factor (TNF)-alpha, interleukin (IL)-6 and (NO) in osteoblasts. |
1(0,0,0,1) | Details |
| 17501665 | Lambert C, Oury C, Dejardin E, Chariot A, Piette J, Malaise M, Merville MP, Franchimont N: Further insights in the mechanisms of interleukin-1beta stimulation of osteoprotegerin in osteoblast-like cells. J Bone Miner Res. 2007 Sep;22(9):1350-61. Post-transcriptional effects were examined by using cycloheximide and actinomycin D. INTRODUCTION: Osteoprotegerin (OPG) plays a key role in the regulation of bone resorption and is stimulated by interleukin (IL)-1beta. |
1(0,0,0,1) | Details |
| 17635918 | Jang BC, Sung SH, Park JG, Park JW, Bae JH, Shin DH, Park GY, Han SB, Suh SI: hydrochloride specifically inhibits COX-2 by preventing COX-2 N-glycosylation and by increasing COX-2 protein turnover in a proteasome-dependent manner. J Biol Chem. 2007 Sep 21;282(38):27622-32. Epub 2007 Jul 16. Remarkably, GS-HCl-mediated decrease in COX-2 molecular mass was associated with inhibition of COX-2 N-glycosylation during translation, as assessed by the effect of tunicamycin, the protein N-glycosylation inhibitor, or of cycloheximide, the translation inhibitor, on COX-2 modification. |
0(0,0,0,0) | Details |