Protein Information

Name ARNT
Synonyms AAAP6077; TANGO; ARNT; C219 reactive peptide; MIA 3; MIA3; UNQ6077; UNQ6077 protein…

Compound Information

Name cycloheximide
CAS

Reference List

PubMed Abstract RScore(About this table)
15837795 Beischlag TV, Perdew GH: ER alpha-AHR-ARNT protein-protein interactions mediate estradiol-dependent transrepression of dioxin-inducible gene transcription. J Biol Chem. 2005 Jun 3;280(22):21607-11. Epub 2005 Apr 18.

Both AHR and ARNT were capable of interacting directly with ER alpha, as ascertained by glutathione S-transferase pull-down. 17Beta-estradiol repressed TCDD-activated Cyp1a1 and Cyp1b1 gene transcription in MCF-7 cells in the presence of cycloheximide, as determined by reverse transcription/real-time PCR.
33(0,1,1,3) Details
16226227 Pollenz RS, Popat J, Dougherty EJ: Role of the carboxy-terminal transactivation domain and active transcription in the ligand-induced and ligand-independent degradation of the mouse Ahb-1 receptor. Biochem Pharmacol. 2005 Nov 25;70(11):1623-33. Epub 2005 Oct 13.

However, AD inhibited TCDD-induced degradation when ARNT expression was restored.
To assess the importance of transactivation domains (TAD), DNA binding and transcription on the degradation of the AH receptor (AHR), Hepa-1 cells were pre-treated with actinomycin D (AD) or cycloheximide (CHX) and exposed to 2,3,7,8 tetrachlorodibenzo-p-dioxin (TCDD).
1(0,0,0,1) Details