| Name | p65 |
|---|---|
| Synonyms | Erythrocyte 65 kDa protein; p65 |
| Name | cycloheximide |
|---|---|
| CAS |
| PubMed | Abstract | RScore(About this table) | |
|---|---|---|---|
| 19398497 | Kang JH, Chang SY, Jang HJ, Kim DB, Ryu GR, Ko SH, Jeong IK, Jo YH, Kim MJ: Exendin-4 inhibits interleukin-1beta-induced iNOS expression at the protein level, but not at the transcriptional and posttranscriptional levels, in RINm5F beta-cells. J Endocrinol. 2009 Jul;202(1):65-75. Epub 2009 Apr 27. Additionally, the cycloheximide chase study demonstrated that EX-4 significantly accelerated iNOS protein degradation. |
0(0,0,0,0) | Details |
| 16581346 | Tamai M, Kawakami A, Tanaka F, Miyashita T, Nakamura H, Iwanaga N, Izumi Y, Arima K, Aratake K, Huang M, Kamachi M, Ida H, Origuchi T, Eguchi K: Significant inhibition of TRAIL-mediated fibroblast-like synovial cell apoptosis by IFN-gamma through JAK/STAT pathway by translational regulation. J Lab Clin Med. 2006 Apr;147(4):182-90. Janus kinase (JAK)-induced phosphorylation of STAT1/3/6, which acts at translational regulation, seemed to be crucial because chemical inhibition of JAK as well as cycloheximide (CHX) abolished both the phosphorylation of STAT1/3/6 and the IFN-gamma-induced inhibitory effect. |
0(0,0,0,0) | Details |
| 18941459 | Diallo JS, Betton B, Parent N, Peant B, Lessard L, Le Page C, Bertrand R, Mes-Masson AM, Saad F: Enhanced killing of androgen-independent prostate cancer cells using inositol hexakisphosphate in combination with proteasome inhibitors. Br J Cancer. 2008 Nov 18;99(10):1613-22. Epub 2008 Oct 21. Although blocking transcription using actinomycin D does not modulate PC3 cell sensitivity to IP6, inhibition of protein translation using cycloheximide has a significant protective effect. |
0(0,0,0,0) | Details |
| 15806306 | Tanaka F, Kawakami A, Tamai M, Nakamura H, Iwanaga N, Izumi Y, Arima K, Aratake K, Huang M, Kamachi M, Ida H, Origuchi T, Eguchi K: IFN-gamma/JAK/STAT pathway-induced inhibition of DR4 and DR5 expression on endothelial cells is cancelled by cycloheximide-sensitive mechanism: novel finding of cycloheximide-regulating death receptor expression. Int J Mol Med. 2005 May;15(5):833-9. |
0(0,0,0,0) | Details |
| 17599377 | Khan S, Liu S, Stoner M, Safe S: Cobaltous chloride and hypoxia inhibit aryl hydrocarbon receptor-mediated responses in breast cancer cells. Toxicol Appl Pharmacol. 2007 Aug 15;223(1):28-38. Epub 2007 May 25. Cotreatment of ZR-75 cells with TCDD plus the protein synthesis inhibitor cycloheximide for 6 h enhanced CYP1A1 expression in cells grown under hypoxia and normoxia. |
0(0,0,0,0) | Details |
| 17178924 | Moreau ME, Bawolak MT, Morissette G, Adam A, Marceau F: Role of nuclear factor-kappaB and protein kinase C signaling in the expression of the kinin B1 receptor in human vascular smooth muscle cells. Mol Pharmacol. 2007 Mar;71(3):949-56. Epub 2006 Dec 18. Various stimulatory compounds that increase receptor mRNA stability only (human and bovine sera, cycloheximide) or that stimulate NF-kappaB nuclear translocation and both mRNA concentration and stability [interleukin (IL)-1beta, phorbol 12- 13- (PMA)] all increased the density of binding sites for the tritiated B1 receptor agonist [3H] -des-Arg9-bradykinin (without change in receptor affinity) in cell-based assays. Small interfering RNA assays indicated that NF-kappaB p65 is necessary for the effective expression of the cell surface B1 receptor under basal or IL-1beta, fetal bovine serum (FBS), or PMA stimulation conditions. |
1(0,0,0,1) | Details |
| 16690986 | Lee KY, Ho SC, Lin HC, Lin SM, Liu CY, Huang CD, Wang CH, Chung KF, Kuo HP: Neutrophil-derived elastase induces TGF-beta1 secretion in human airway smooth muscle via NF-kappaB pathway. Am J Respir Cell Mol Biol. 2006 Oct;35(4):407-14. Epub 2006 May 11. This induction was inhibited by actinomycin D (5 mM), cycloheximide (5 mM), and NF-kappaB inhibitors, including pyrrolidine dithiocarbamate (PDTC, 1 mM), (2.5 mM), and salyicylate (2.5 mM). Stimulation with NE was rapidly followed by association of IRAK with MyD88, phosphorylation of IkappaBalpha, and nuclear translocation of p65 with increased transactivation activity. |
1(0,0,0,1) | Details |
| 16822952 | Basuroy S, Bhattacharya S, Tcheranova D, Qu Y, Regan RF, Leffler CW, Parfenova H: HO-2 provides endogenous protection against oxidative stress and apoptosis caused by TNF-alpha in cerebral vascular endothelial cells. Am J Physiol Cell Physiol. 2006 Nov;291(5):C897-908. Epub 2006 Jul 5. In CMVEC from mice and newborn pigs, 15 ng/ml TNF-alpha alone, or with 10 microg/ml cycloheximide (CHX) caused apoptosis detected by nuclear translocation of p65 NF-kappaB, caspase-3 activation, DNA fragmentation, cell-cell contact destabilization, and cell detachment. |
31(0,1,1,1) | Details |
| 15870875 | Kwon YK, Jun JM, Shin SW, Cho JW, Suh SI: decreases cell proliferation rates through BTG2-mediated cyclin D1 down-regulation in U937 cells. Int J Oncol. 2005 Jun;26(6):1597-603. Nuclear translocation of p65 NF-kappaB is involved in the expression of cyclin D1 mRNA. |
2(0,0,0,2) | Details |
| 18163503 | Bauge C, Attia J, Leclercq S, Pujol JP, Galera P, Boumediene K: Interleukin-1beta up-regulation of Smad7 via NF-kappaB activation in human chondrocytes. Arthritis Rheum. 2008 Jan;58(1):221-6. METHODS: Human articular chondrocytes were treated with IL-1beta in the presence of TGFbeta1, pyrrolidine dithiocarbamate (a repressor of the NF-kappaB pathway), or cycloheximide. In addition, transient transfections of p65 expression vector or p65-targeted short hairpin RNA were performed to define the effect of NF-kappaB on Smad7 expression. |
2(0,0,0,2) | Details |
| 18719026 | Buzzelli MD, Nagarajan M, Radtka JF, Shumate ML, Navaratnarajah M, Lang CH, Cooney RN: Nuclear factor-kappaB mediates the inhibitory effects of tumor necrosis factor-alpha on growth hormone-inducible gene expression in liver. Endocrinology. 2008 Dec;149(12):6378-88. Epub 2008 Aug 21. Cycloheximide did not antagonize the inhibitory effects of TNF on GH-inducible IGF-I expression. Cotransfection of CWSV-1 cells with expression vectors for p65 alone or p50 and p65 together inhibited GH-inducible Spi 2.1 and IGF-I promoter activity. |
1(0,0,0,1) | Details |
| 17501665 | Lambert C, Oury C, Dejardin E, Chariot A, Piette J, Malaise M, Merville MP, Franchimont N: Further insights in the mechanisms of interleukin-1beta stimulation of osteoprotegerin in osteoblast-like cells. J Bone Miner Res. 2007 Sep;22(9):1350-61. NF-kappaB function was studied using BAY 11-7085 and by siRNA transfection to inhibit p65 synthesis. Post-transcriptional effects were examined by using cycloheximide and actinomycin D. |
1(0,0,0,1) | Details |
| 16637064 | Choi W, Proctor L, Xia Q, Feng Y, Gerner EW, Chiao PJ, Hamilton SR, Zhang W: Inactivation of IkappaB contributes to transcriptional activation of / N (1)-acetyltransferase. Mol Carcinog. 2006 Sep;45(9):685-93. CHX quickly eliminated the IkappaB protein in the cells and increased the levels of the two subunits of NFkappaB, p65 and p50, in the nucleus. |
1(0,0,0,1) | Details |
| 19787057 | Sung MH, Salvatore L, De Lorenzi R, Indrawan A, Pasparakis M, Hager GL, Bianchi ME, Agresti A: Sustained oscillations of NF-kappaB produce distinct genome scanning and gene expression profiles. PLoS One. 2009 Sep 29;4(9):e7163. To examine real time dynamics in living cells, we monitored NF-kappaB activities at multiple timescales using GFP-p65 knock-in mouse embryonic fibroblasts. |
1(0,0,0,1) | Details |