Protein Information

Name RNase A
Synonyms HP RNase; Ribonuclease; Ribonuclease A; Pancreatic ribonuclease; Pancreatic ribonuclease precursor; RIB 1; RIB1; RNASE1…

Compound Information

Name cycloheximide
CAS

Reference List

PubMed Abstract RScore(About this table)
17178387 Januchowski R, Jagodzinski PP: Trichostatin A down-regulates ZAP-70, LAT and SLP-76 content in Jurkat T cells. Int Immunopharmacol. 2007 Feb;7(2):198-204. Epub 2006 Oct 17.

Employing the protein biosynthesis inhibitor cycloheximide, we demonstrated the involvement of RNase and/or mRNA stabilization protein in ZAP-70, LAT and SLP-76 mRNAs stabilization.
31(0,1,1,1) Details
18708044 Lin MD, Jiao X, Grima D, Newbury SF, Kiledjian M, Chou TB: Drosophila processing bodies in oogenesis. Dev Biol. 2008 Oct 15;322(2):276-88. Epub 2008 Aug 5.

In stage 2-6 oocytes, dDcp1 bodies appear to be distinct from previously characterized P-bodies since they are insensitive to cycloheximide and RNase A treatments.
6(0,0,1,1) Details
19584246 Taddeo B, Zhang W, Roizman B: The virion-packaged endoribonuclease of herpes simplex virus 1 cleaves mRNA in polyribosomes. Proc Natl Acad Sci U S A. 2009 Jul 21;106(29):12139-44. Epub 2009 Jul 7.

Specifically, in contrast to the events in uninfected cells or cells infected with a mutant lacking the RNase, in wild-type virus-infected cells mRNA of housekeeping genes exemplified by GAPDH is degraded rapidly, whereas mRNAs containing AU elements are cleaved and the 5' cleavage product of these RNAs persists for many hours.
These P-bodies were also preset in cycloheximide (CHX)-treated cells but not in either treated or untreated uninfected cells or cells infected with the RNase minus mutant.
2(0,0,0,2) Details
16514669 Vogt RR, Unda R, Yeh LC, Vidro EK, Lee JC, Tsin AT: Bone morphogenetic protein-4 enhances vascular endothelial growth factor secretion by human retinal pigment epithelial cells. J Cell Biochem. 2006 Aug 1;98(5):1196-202.

Results from RNase protection assays (RPAs) show that RPE from non-diabetic human donors and from adult retinal pigment epithelium-19 (ARPE-19) cells expressed significant bone morphogenetic protein-4 (BMP-4) message.
Addition of cycloheximide (CHX) or actinomycin-D (ACT) significantly reduced VEGF secretion from cells treated with BMP-4, suggesting that the BMP-4-induced secretion of VEGF requires new RNA and protein synthesis.
1(0,0,0,1) Details
18321792 Paronetto MP, Bianchi E, Geremia R, Sette C: Dynamic expression of the RNA-binding protein Sam68 during mouse pre-implantation development. Gene Expr Patterns. 2008 May;8(5):311-22. Epub 2008 Feb 2.

These granules contained RNA and were dissolved by treatment with RNase A.
However, we found that inhibition of mRNA translation by either cycloheximide or puromycin in one-cell embryos caused the accumulation of Sam68 in cytoplasmic granules.
1(0,0,0,1) Details
18664117 Grigor'ev AA, Bulycheva TI, Sheval' EV, Kalinina IA, Zatsepina OV: [The cytological indicators of the overall suppression of protein synthesis as revealed by staining with a new monoclonal antibody]. Tsitologiia. 2008;50(4):338-46.


A3 antigen has an intriguing property, namely, an ability to migrate from the nucleolus to the nucleoplasm upon inhibition of protein synthesis with anisomycin, puromycin or cycloheximide.
0(0,0,0,0) Details