| 16222729 |
Dietrich M, Grundmann L, Kurr K, Valinotto L, Saussele T, Schmid RD, Lange S: Recombinant production of human microsomal cytochrome P450 2D6 in the methylotrophic yeast Pichia pastoris. Chembiochem. 2005 Nov;6(11):2014-22.
The cDNAs encoding human CYP2D6 and human NADPH-P450 oxidoreductase (CPR) were transformed into the methylotrophic yeast Pichia pastoris and expressed with control of the methanol-inducible AOX1 promoter.
Microsomes containing human CPR and CYP2D6 converted different substrates, such as 3-cyano-7-ethoxycoumarin, parathion and dextrometorphan. |
1(0,0,0,1) |
Details |