| 15952742 |
Sprung R, Nandi A, Chen Y, Kim SC, Barma D, Falck JR, Zhao Y: Tagging-via-substrate strategy for probing O-GlcNAc modified proteins. J Proteome Res. 2005 May-Jun;4(3):950-7.
Here, we report a strategy for the detection, isolation, and profiling of O-linked N-acetylglucosamine (O-GlcNAc) modified proteins, which involves three steps: metabolic labeling of cells with an unnatural GlcNAc analogue, peracetylated azido-GlcNAc; chemoselective conjugation of azido-GlcNAc modified proteins via the Staudinger ligation, which is specific between phosphine and azide, using a biotinylated phosphine capture reagent; and detection and affinity purification of the resulting conjugated O-GlcNAc modified proteins. |
84(1,1,1,4) |
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