Protein Information

Name transferrins
Synonyms Beta 1 metal binding globulin; PRO1400; PRO1557; PRO2086; Serotransferrin; Serotransferrin precursor; Siderophilin; TF…

Compound Information

Name phosphine
CAS phosphine

Reference List

PubMed Abstract RScore(About this table)
12781465 Thevis M, Loo RR, Loo JA: Mass spectrometric characterization of transferrins and their fragments derived by reduction of disulfide bonds. J Am Soc Mass Spectrom. 2003 Jun;14(6):635-47.

Incubation of transferrin with reductants, such as dithiothreitol (DTT) or tris (2-carboxyethyl)-phosphine (TCEP), yields an increase in multiple charging observed by ESI-MS and an increase in molecular weight consistent with disulfide reduction.
37(0,1,1,7) Details
19454248 Warder SE, Tucker LA, Strelitzer TJ, McKeegan EM, Meuth JL, Jung PM, Saraf A, Singh B, Lai-Zhang J, Gagne G, Rogers JC: Reducing agent-mediated precipitation of high-abundance plasma proteins. Anal Biochem. 2009 Apr 15;387(2):184-93. Epub 2009 Jan 19.

This report describes a process that rapidly and reproducibly precipitates high-abundance disulfide-rich proteins, including albumin and transferrin, from serum and plasma.
A low volume of concentrated reducing agent, viz. dithiothreitol (DTT) or tris (2-carboxyethyl) phosphine (TCEP), was added directly to plasma followed by a brief incubation at ambient temperature.
1(0,0,0,1) Details
15621682 Visser CC, Voorwinden LH, Harders LR, Eloualid M, van Bloois L, Crommelin DJ, Danhof M, de Boer AG: Coupling of metal containing homing devices to liposomes via a maleimide linker: use of TCEP to stabilize thiol-groups without scavenging metals. J Drug Target. 2004;12(9-10):569-73.


Tris (2-carboxyethyl) phosphine (TCEP) hydrochloride is a sulfhydryl reductant that is often used in protein biochemistry.
0(0,0,0,0) Details