Protein Information

Name C18
Synonyms B1; D1; BBS 9; BBS9; BBS9 GENE; Bardet Biedl syndrome 9 protein; C18; PTH responsive osteosarcoma B1 protein…

Compound Information

Name butylamine
CAS 2-butanamine

Reference List

PubMed Abstract RScore(About this table)
7127747 Tasset JJ, Hassan FM: Liquid-chromatographic determination of amoxapine and 8-hydroxyamoxapine in human serum. Clin Chem. 1982 Oct;28(10):2154-7.

We used a mu-Bondapak C18 reversed-phase column and a mobile phase of acetonitrile/water (74/26 by vol) plus 26 microL of n-butylamine per liter.
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3805205 Lambrechts M, Tonnesen F, Rasmussen KE: Profiling of impurities in illicit amphetamine samples by high-performance liquid chromatography using column switching. J Chromatogr. 1986 Nov 21;369(2):365-77.

After washing for 1.5 min, a six-port valve is switched and an acetonitrile-0.2 M butylamine in water (pH 8) gradient elutes the impurities from the extraction column on to a C18 analytical column where they are separated.
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10735303 Perez-Urquiza M, Prat MD, Beltran JL: Determination of sulphonate dyes in water by ion-interaction high-performance liquid chromatography. J Chromatogr A. 2000 Feb 25;871(1-2):227-34.

An RP-ODS stationary phase is used, and the mobile phase contains an acetonitrile-phosphate buffer (27:73, v/v) mixture at pH 6.7, containing 2.4 mM butylamine as ion-interaction reagent.
Spiked tap water samples (100 ml), containing different concentration levels (0.3-1.2 microg/l) of the dyes were analyzed after acidification (pH 3) and preconcentration in disposable solid-phase extraction C18 cartridges.
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15117076 Meseguer Lloret S, Molins Legua C, Verdu Andres J, Campins Falco P: Sensitive determination of aliphatic amines in water by high-performance liquid chromatography with chemiluminescence detection. J Chromatogr A. 2004 Apr 30;1035(1):75-82.

Analytes are preconcentrated and dansylated on solid sorbents (C18 solid-phase extraction cartridges).
The method has been applied to the quantification or screening of several aliphatic amines: methylamine, ethylamine, butylamine, diethylamine, pentylamine and hexylamine.
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6636257 Beierle FA, Hubbard RW: Liquid chromatographic separation of antidepressant drugs: II. Ther Drug Monit. 1983;5(3):293-301.

Serum (1.0 ml collected in plastic) extraction was by Bond-Elut C18 columns.
The mobile phase was absolute ethanol-acetonitrile-tert-butylamine (98:2:0.05, vol/vol), and the flow rate was 2.0 ml/min.
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7894673 Nieto C, Ramis J, Conte L, Fernandez JM, Forn J: On-line fully automated solid-phase extraction-liquid chromatography analysis of 1,2-dihydro-4-(1,2-dihydro-2-oxo-1-pyridyl)-2,2-dimethyl-1-oxonaphthale ne-6- carbonitrile (UR-8225), a new potassium channel opener, in plasma samples. J Chromatogr B Biomed Appl. 1994 Nov 18;661(2):319-25.

On-line solid-phase extraction was performed with disposable C18 cartridges.
After clean up, the samples were eluted and transferred onto an RP-18 analytical column, where separation was performed with a mobile phase of acetonitrile-10 mM di-n-butylamine phosphate (28:72, v/v).
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10949476 Sasaki T, Iida T, Nambara T: High-performance ion-pair chromatographic behaviour of conjugated bile acids with di-n-butylamine acetate. J Chromatogr A. 2000 Aug 4;888(1-2):93-102.

The anionic conjugated bile acids were chromatographed on a C18, reversed-phase ion-pair column, eluting with methanol-water (65:35, v/v) containing 5 mM of DBAA as a counter ion.
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6636256 Beierle FA, Hubbard RW: Liquid chromatographic separation of antidepressant drugs: I. Ther Drug Monit. 1983;5(3):279-92.

Extraction of the 1.0-ml serum samples (collected in plastic) was done with Bond-Elut C18 columns.
The mobile phase was absolute ethanol-acetonitrile-tert-butylamine (98:2:0.05, vol/vol/vol).
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3571404 Kwong EC, Shen DD: Versatile isocratic high-performance liquid chromatographic assay for propranolol and its basic, neutral and acidic metabolites in biological fluids. J Chromatogr. 1987 Mar 6;414(2):365-79.

Chromatographic separation of the metabolites in the different extracts was achieved on a reversed-phase C18 column, using a single isocratic mobile phase consisting of 0.044 M pH 2.7 phosphate buffer, tetrahydrofuran, methanol and acetonitrile, with the addition of n-butylamine as a competing base to control retention volume and peak shape.
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9306670 Rothe M, Pragst F, Spiegel K, Harrach T, Fischer K, Kunkel J: Hair concentrations and self-reported abuse history of 20 amphetamine and ecstasy users. Forensic Sci Int. 1997 Sep 19;89(1-2):111-28.

Hair samples of 20 volunteers of the techno-music scene, who more or less regularly consumed ecstasy tablets and speed and anonymously reported their abuse history, were analyzed in one to seven 3 cm segments for amphetamine (A), methamphetamine (MA), methylenedioxyamphetamine (MDA), methylenedioxymethamphetamine (MDMA), methylenedioxyethamphetamine (MDE) and N-methyl-1-(1,3-benzodioxol-5-yl)-2-butylamine (MBDB) by digestion in 1 M NaOH, subsequent extraction with C18 Bond Elut columns, derivatization with pentafluoropropionyl anhydride and GC/MS-SIM measurements using deuterated standards of A, MA, MDA and MDMA.
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1952052 Anumula KR, Taylor PB: Quantitative determination of phenyl isothiocyanate-derivatized amino sugars and amino sugar alcohols by high-performance liquid chromatography. Anal Biochem. 1991 Aug 15;197(1):113-20.

The mobile phase containing n-butylamine, in conjunction with a C18 stationary phase, mimics the conditions for the separation of carbohydrates on an amino-bonded column.
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8010017 Luo SR, Li T, Yang JS: [Determination of gelsemium alkaloids by RP-HPLC] . Yao Xue Xue Bao. 1993;28(9):695-8.

In this report a RP column of C18 and the mobile phase methanol--water--n-butylamine (78:22:0.1 V/V) were employed.
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8454692 Plomp TA, Buijs MJ: High-performance liquid chromatographic method for the simultaneous determination of pentisomide and its major metabolite N-desisopropylpentisomide in plasma, urine and tissues using solid-phase extraction. J Chromatogr. 1993 Jan 29;612(1):123-35.

Separation is performed using a Nova-Pak C18 4 microns particle size column operating in combination with radial compression separation unit and a methanol-water-di-sec.-butylamine-phosphoric acid (40:60:0.5:0.2, v/v) pH 3.5 mobile phase with ultraviolet detection at 258 nm.
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