Protein Information

Name DTNB
Synonyms Beta dystrobrevin; DTN B; DTNB; Dystrobrevin beta; Dystrobrevin betas

Compound Information

Name propoxur
CAS

Reference List

PubMed Abstract RScore(About this table)
19830865 Li SG: [Differences of acetylcholinesterase level in variety classes and strains of Culex pipiens pallens]. Zhejiang Da Xue Xue Bao Yi Xue Ban. 2009 Sep;38(5):511-4.

METHODS: AChE insensitivity of single mosquito was determined, using acetythiocholine iodide (ATch) as the substrate, 5,5'-dithio-bis (2-nitrobenzoic acid) (DTNB) as the developer, and propoxur as the inhibitor.
31(0,1,1,1) Details
18965297 Quintero MC, Silva M, Perez-Bendito D: Enzymatic determination of N-methylcarbamate pesticides at the nanomolar level by the stopped-flow technique. Talanta. 1991 Nov;38(11):1273-7.

It is based on their inhibitory effect on electric eel acetylcholinesterase and the use of 5,5'-dithiobis (2-nitrobenzoic) acid (DTNB) as chromogenic reagent for the thiocholine released from the acetylthiocholine iodide substrate.
Carbaryl, propoxur and carbofuran can be determined at concentrations in the ranges 6.5-120, 2-15 and 0.1-5.0 ng/ml, respectively, by the proposed method.
2(0,0,0,2) Details
1488701 Lee HL, Abimbola O, Singh KI: Determination of insecticide susceptibility in Culex quinquefasciatus Say adults by rapid enzyme microassays. Southeast Asian J Trop Med Public Health. 1992 Sep;23(3):458-63.

In the insensitive acetylcholinesterase (AChE) test, acetylthiocholine iodide (ACTH) and 5,5-dithiobis-(2-nitrobenzoic acid) (DTNB) were used as substrate and coupling agent respectively.
Test results showed that the color intensity decreased as increasing concentrations of propoxur were added, thereby confirming the susceptibility of the enzyme to inhibitor.
1(0,0,0,1) Details