Protein Information

Name soluble epoxide hydrolase
Synonyms CEH; Epoxide hydratase; Cytosolic epoxide hydrolase; EPHX 2; EPHX2; Epoxide hydrolase 2; SEH; Soluble epoxide hydrolase…

Compound Information

Name nitrostyrene
CAS 4-(2-nitro-1-propenyl)phenyl thiocyanate

Reference List

PubMed Abstract RScore(About this table)
3218741 Wixtrom RN, Hammock BD: Continuous spectrophotometric assays for cytosolic epoxide hydrolase. . Anal Biochem. 1988 Oct;174(1):291-9.

The assays are applicable to whole tissue homogenates as well as purified enzyme preparations. p-Nitrostyrene oxide and p-nitrophenyl glycidyl ether were also examined and found to be very poor substrates for cytosolic epoxide hydrolase from mouse liver.
85(1,1,1,5) Details
19452475 Naundorf A, Melzer G, Archelas A, Furstoss R, Wohlgemuth R: Influence of pH on the expression of a recombinant epoxide hydrolase in Aspergillus niger. Biotechnol J. 2009 May;4(5):756-65.

The filamentous fungus Aspergillus niger was investigated in relation to its ability to produce a soluble epoxide hydrolase (EH) (E.C. 3.3.2.3) belonging to the microsomal EH family.
The production of EH was highest (1000 U/L for p-nitrostyrene oxide) under acidic fermentation conditions (pH value of about 3).
1(0,0,0,1) Details
6185139 Wang P, Meijer J, Guengerich FP: Purification of human liver cytosolic epoxide hydrolase and comparison to the microsomal enzyme. Biochemistry. 1982 Nov 9;21(23):5769-76.

Purified cytosolic TESO hydrolase catalyzes the hydrolysis of cis-8-ethylstyrene 7,8-oxide 10 times more rapidly than does the microsomal enzyme, catalyzes the hydrolysis of TESO and trans-stilbene oxide as rapidly as the microsomal enzyme, but catalyzes the hydrolysis of styrene 7,8-oxide, p-nitrostyrene 7,8-oxide, and naphthalene 1,2-oxide much less effectively than does the microsomal enzyme.
1(0,0,0,1) Details