| Name | single chain Fv |
|---|---|
| Synonyms | SCFV; Single chain Fv; scFv; single chain Fv fragment; Single chain Fvs; scFvs; single chain Fv fragments |
| Name | gibberellins |
|---|---|
| CAS | gibberellins |
| PubMed | Abstract | RScore(About this table) | |
|---|---|---|---|
| 10361695 | Shimada N, Suzuki Y, Nakajima M, Conrad U, Murofushi N, Yamaguchi I: Expression of a functional single-chain antibody against GA24/19 in transgenic tobacco. Biosci Biotechnol Biochem. 1999 Apr;63(4):779-83. The dwarf phenotype of the transgenic plants expressing the single-chain Fv protein, together with the preliminary analytical data indicating a decreased level of A1 in the dwarf transgenics, suggested that the single-chain Fv decreased the concentration of bioactive gibberellins by trapping and inhibiting the metabolism of A24 and/or A19 to A4 and/or A1. |
34(0,1,1,4) | Details |
| 18636270 | Urakami E, Yamaguchi I, Asami T, Conrad U, Suzuki Y: Immunomodulation of biosynthesis using an anti-precursor antibody confers -deficient phenotypes. Planta. 2008 Oct;228(5):863-73. Epub 2008 Jul 18. In this study, a single-chain antibody (scFv) against GA (24), a precursor GA, was utilized to repress the biosynthesis of bioactive gibberellins. |
11(0,0,1,6) | Details |
| 18282174 | Suzuki Y, Mizuno T, Urakami E, Yamaguchi I, Asami T: Immunomodulation of bioactive confers -deficient phenotypes in plants. Plant Biotechnol J. 2008 May;6(4):355-67. Epub 2008 Feb 14. This method was applied to plants to repress the function of gibberellins (GAs), a class of phytohormones responsible for plant elongation, by anti-bioactive GA antibodies. Two different antibodies were produced in Arabidopsis as single-chain variable fragment (scFv) fused to green fluorescent protein (GFP) with four different subcellular localizations: endoplasmic reticulum (ER), cytosol, apoplastic space or the outer surface of the plasma membrane. |
5(0,0,0,5) | Details |
| 10361677 | Suzuki Y, Shimada N, Niwa R, Yokoi M, Nakajima M, Murofushi N, Yamaguchi I: Preparation and application of anti-idiotypic antibody against anti- A4 antibody. Biosci Biotechnol Biochem. 1999 Apr;63(4):648-54. A monoclonal anti-idiotypic antibody was raised against anti- A4 (GA4) antibody, which recognizes biologically active gibberellins such as and GA4 specifically. The single-chain Fv (scFv) gene of the anti-idiotypic antibody was constructed, and scFv expressed in E. coli showed binding activity to anti-GA4 antibody. |
2(0,0,0,2) | Details |
| 15784991 | Suzuki Y, Ito S, Otsuka K, Iwasawa E, Nakajima M, Yamaguchi I: Preparation of functional single-chain antibodies against bioactive gibberellins by utilizing randomly mutagenized phage-display libraries. Biosci Biotechnol Biochem. 2005 Mar;69(3):610-9. Single-chain antibody libraries with random mutations were constructed from two independent anti-bioactive monoclonal antibody lines in a phagemid vector, so that the mutagenized scFvs were expressed in a phage-displayed form upon helper phage infection. |
2(0,0,0,2) | Details |