Protein Information

Name Ldh c
Synonyms L lactate dehydrogenase C chain; LDH testis subunit; LDH 3; LDH C; LDH X; LDH3; LDHC; LDHX…

Compound Information

Name oxamate
CAS hexyl 2-(diethylamino)-2-oxoacetate

Reference List

PubMed Abstract RScore(About this table)
213050 Kolk AH, van Kuyk L, Boettcher B: Isolation of human lactate dehydrogenase isoenzyme X by affinity chromatography. Biochem J. 1978 Sep 1;173(3):767-71.

Human isoenzyme LDH-X (lactate dehydrogenase isoenzyme X) was isolated from seminal fluid of frozen semen samples by affinity chromatography by using oxamate-Sepharose and AMP-Sepharose.
33(0,1,1,3) Details
182529 Spielmann H, Eibs HG, Mentzel C: Rapid purification of lactate dehydrogenase X from mouse testes by two steps of affinity chromatography on oxamate-sepharose. Experientia. 1976 Aug 15;32(8):1085-6.

At concentrations of 200 muM NADH and 0.5 M NaCl LDH-X is separated from the other LDH isozymes of mouse testes on oxamate-sepharose.
7(0,0,1,2) Details
7358131 Toowicharanont P, Svasti J: A logical approach to the isolation of lactate dehydrogenase isozyme X from human testes: a general rationale for the isolation of homotetrameric LDH isozymes. Experientia. 1980 Jan 15;36(1):37-8.

Immunoadsorbent and oxamate-Sepharose chromatography were used to isolate electrophoretically homogeneous LDH-X from human testes with a final specific activity of 125 IU/mg and good yields: other applications of this appraoch are discussed.
6(0,0,1,1) Details
1996957 LeVan KM, Goldberg E: Properties of human testis-specific lactate dehydrogenase expressed from Escherichia coli. Biochem J. 1991 Feb 1;273 ( Pt 3):587-92.

The Ldh-c open reading frame was constructed as a cassette for production of the native protein.
Human LDH-C4 was purified over 200-fold from litre cultures of cells by AMP and oxamate affinity chromatography to a specific activity of 106 units/mg.
2(0,0,0,2) Details