Protein Information

Name aldehyde dehydrogenase (protein family or complex)
Synonyms aldehyde dehydrogenase; aldehyde dehydrogenases

Compound Information

Name eugenol
CAS 2-methoxy-4-(2-propen-1-yl)phenol

Reference List

PubMed Abstract RScore(About this table)
12200281 Overhage J, Steinbuchel A, Priefert H: Biotransformation of eugenol to ferulic acid by a recombinant strain of Ralstonia eutropha H16. Appl Environ Microbiol. 2002 Sep;68(9):4315-21.

The gene loci ehyAB, calA, and calB, encoding eugenol hydroxylase, coniferyl alcohol dehydrogenase, and coniferyl aldehyde dehydrogenase, respectively, which are involved in the first steps of eugenol catabolism in Pseudomonas sp. strain HR199, were amplified by PCR and combined to construct a catabolic gene cassette.
37(0,1,2,2) Details
9721273 Achterholt S, Priefert H, Steinbuchel A: Purification and characterization of the coniferyl aldehyde dehydrogenase from Pseudomonas sp. J Bacteriol. 1998 Sep;180(17):4387-91.

The coniferyl aldehyde dehydrogenase (CALDH) of Pseudomonas sp. strain HR199 (DSM7063), which catalyzes the NAD+-dependent oxidation of coniferyl aldehyde to ferulic acid and which is induced during growth with eugenol as the carbon source, was purified and characterized.
33(0,1,1,3) Details
14602615 Overhage J, Steinbuchel A, Priefert H: Highly efficient biotransformation of eugenol to ferulic acid and further conversion to vanillin in recombinant strains of Escherichia coli. Appl Environ Microbiol. 2003 Nov;69(11):6569-76.

The vaoA gene from Penicillium simplicissimum CBS 170.90, encoding vanillyl alcohol oxidase, which also catalyzes the conversion of eugenol to coniferyl alcohol, was expressed in Escherichia coli XL1-Blue under the control of the lac promoter, together with the genes calA and calB, encoding coniferyl alcohol dehydrogenase and coniferyl aldehyde dehydrogenase of Pseudomonas sp. strain HR199, respectively.
31(0,1,1,1) Details
16421716 Plaggenborg R, Overhage J, Loos A, Archer JA, Lessard P, Sinskey AJ, Steinbuchel A, Priefert H: Potential of Rhodococcus strains for biotechnological vanillin production from ferulic acid and eugenol. Appl Microbiol Biotechnol. 2006 Oct;72(4):745-55. Epub 2006 Jan 19.

To establish eugenol bioconversion in PD630, the vanillyl alcohol oxidase gene (vaoA) from Penicillium simplicissimum CBS 170.90 was expressed in PD630 together with coniferyl alcohol dehydrogenase (calA) and coniferyl aldehyde dehydrogenase (calB) genes from Pseudomonas sp.
6(0,0,1,1) Details
10616715 Overhage J, Priefert H, Rabenhorst J, Steinbuchel A: Biotransformation of eugenol to vanillin by a mutant of Pseudomonas sp. strain HR199 constructed by disruption of the vanillin dehydrogenase (vdh) gene. Appl Microbiol Biotechnol. 1999 Nov;52(6):820-8.

Characterization of the purified VDH-II revealed the identify of this enzyme with the recently characterized coniferyl-aldehyde dehydrogenase.
1(0,0,0,1) Details