Protein Information

Name COMT
Synonyms COMT; COMT protein; Catechol O methyltransferase; Catechol O methyltransferase isoform MB COMT; MB COMT; COMT proteins; Catechol O methyltransferases; Catechol O methyltransferase isoform MB COMTs

Compound Information

Name eugenol
CAS 2-methoxy-4-(2-propen-1-yl)phenol

Reference List

PubMed Abstract RScore(About this table)
10415125 Wang J, Pichersky E: Identification of specific residues involved in substrate discrimination in two plant O-methyltransferases. Arch Biochem Biophys. 1999 Aug 1;368(1):172-80.

While IEMT and COMT (which methylate eugenol/isoeugenol and caffeic acid/5-hydroxyferulic acid, respectively) share 83% identity at the amino acid level, they have distinct substrate specificity and methylation regiospecificity.
45(0,1,3,5) Details
16233496 Wu S, Watanabe N, Mita S, Ueda Y, Shibuya M, Ebizuka Y: Two O-methyltransferases isolated from flower petals of Rosa chinensis var. spontanea involved in scent biosynthesis. J Biosci Bioeng. 2003;96(2):119-28.

RcOMT1 efficiently methylated eugenol and isoeugenol to yield volatile methyleugenol and isomethyleugenol, respectively.
In contrast, RcOMT2, which shows 94% similarity with caffeic acid O-methyltransferase (COMT) of Prunus amygdalus, was expressed in all tissues tested and had the highest activity with caffeic acid, a typical substrate for COMT.
2(0,0,0,2) Details
9439593 Wang J, Pichersky E: Characterization of S-adenosyl-L-methionine:(iso) eugenol O-methyltransferase involved in floral scent production in Clarkia breweri. Arch Biochem Biophys. 1998 Jan 1;349(1):153-60.

C. breweri IEMT was copurified with caffeic acid O-methyltransferase (COMT) from petals and purified to homogeneity from a bacterial expression system.
2(0,0,0,2) Details