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Proffer TJ, Berardi R, Ma Z, Nugent JE, Ehret GR, McManus PS, Jones AL, Sundin GW: Occurrence, Distribution, and Polymerase Chain Reaction-Based Detection of Resistance to Sterol Demethylation Inhibitor Fungicides in Populations of Blumeriella jaapii in Michigan. Phytopathology. 2006 Jul;96(7):709-17.
In one survey, 137 isolates from sites with different DMI histories (no known history, mixed or alternated with other fungicides, and exclusive use) were tested against 12 concentrations of fenbuconazole, tebuconazole, myclobutanil, and fenarimol.
The species-specific primer pair (Bj-F and Bj-R) based on introns in the CYP51 gene of B. jaapii, and the DMI (R)-specific primer pair (DMI-R-Bj-F and DMI-R-Bj-R) based on an insert found upstream of CYP51 in all DMI (R) isolates, provided an accurate and rapid method for detecting DMI (R) B. jaapii. |
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