Protein Information

Name hemoglobin (protein family or complex)
Synonyms Hemoglobin; Hemoglobins

Compound Information

Name potassium thiocyanate
CAS potassium thiocyanate

Reference List

PubMed Abstract RScore(About this table)
3464939 Vinogradov SN, Lugo SD, Mainwaring MG, Kapp OH, Crewe AV: Bracelet protein: a quaternary structure proposed for the giant extracellular hemoglobin of Lumbricus terrestris. Proc Natl Acad Sci U S A. 1986 Nov;83(21):8034-8.

The complete dissociation of the hexagonal bilayer structure of Lumbricus terrestris hemoglobin (3900 kDa) at neutral pH, in the presence of urea, guanidine hydrochloride, sodium perchlorate, potassium thiocyanate, sodium phosphotungstate, and sodium phosphomolybdate, followed by gel filtration at neutral pH on Sephacryl S-200 or Superose 6, produced two fragments, II (65 kDa) and III (17 kDa); NaDodSO4/polyacrylamide gel electrophoresis showed that peak II consisted of subunits D1 (31 kDa, chain V), D2 (37 kDa, chain VI), and T (50 kDa, disulfide-bonded trimer of chains II, III, and IV) and that peak II consisted of subunit M (16 kDa, chain I).
40(0,1,2,5) Details
6484780 de Pablos Gallego JM, Cano Trapero R: [Experience with a simple microchromatographic method for hemoglobin A2 quantification (Hb a2)] Sangre. 1984;29(3):314-20.

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12943506 Titov VY, Petrenko YM: Nitrite-catalase interaction as an important element of nitrite toxicity. Biochemistry. 2003 Jun;68(6):627-33.


This was shown by the example of nitrite-induced hemoglobin oxidation.
1(0,0,0,1) Details
9086302 Gella FJ, Gubern G, Vidal R, Canalias F: Determination of total and pancreatic alpha-amylase in human serum with 2-chloro-4-nitrophenyl-alpha-D-maltotrioside as substrate. Clin Chim Acta. 1997 Mar 18;259(1-2):147-60.

The selected reaction mixture contains 50 mmol/l 2-(N-morpholino) ethanesulfonic acid buffer at pH 6.1 (37 degrees C), 300 mmol/l sodium chloride, 5 mmol/l calcium chloride and 450 mmol/l potassium thiocyanate.
The method shows the absence of a lag phase period, is sensitive and precise, has a large analytical range and is free from interference by hemoglobin, bilirubin and triglycerides.
1(0,0,0,1) Details