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Gumber S, Taylor DL, Whittington RJ: Protein extraction from Mycobacterium avium subsp. paratuberculosis: Comparison of methods for analysis by sodium dodecyl sulphate polyacrylamide gel electrophoresis, native PAGE and surface enhanced laser desorption/ionization time of flight mass spectrometry. J Microbiol Methods. 2007 Jan;68(1):115-27. Epub 2006 Aug 17.
However, potassium thiocyanate was the most effective buffer, leading to detection of the greatest number of protein peaks on the immobilized metal affinity chromatography (IMAC) surface. |
81(1,1,1,1) |
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