Protein Information

Name thrombin
Synonyms Coagulation factor II; Coagulation factor II variant; F2; F2 protein; F2 protein precursor; Factor II; PT; Prothrombin…

Compound Information

Name sodium azide
CAS sodium azide

Reference List

PubMed Abstract RScore(About this table)
11354246 Hedin HL, Nilsson L, Fowler CJ: Effects of staurosporine, U-73122, wortmannin, 4-hydroxynonenal and sodium azide upon the release of secreted beta-amyloid precursor protein from human platelets in response to thrombin stimulation. Mol Cell Biochem. 2001 Mar;219(1-2):145-52.
166(2,2,2,6) Details
18845432 Chabot V, Cuerrier CM, Escher E, Aimez V, Grandbois M, Charette PG: Biosensing based on surface plasmon resonance and living cells. Biosens Bioelectron. 2009 Feb 15;24(6):1667-73. Epub 2008 Aug 26.

Specifically, we evaluated three types of stimulation: response to an endotoxin (lipopolysaccharides), a chemical toxin (sodium azide) and a physiological agonist (thrombin).
82(1,1,1,2) Details
17147953 Okawara M, Katsuki H, Kurimoto E, Shibata H, Kume T, Akaike A: Resveratrol protects dopaminergic neurons in midbrain slice culture from multiple insults. Biochem Pharmacol. 2007 Feb 15;73(4):550-60. Epub 2006 Nov 9.

Resveratrol also provided concentration-dependent neuroprotective effects against sodium azide, a mitochondrial complex IV inhibitor, and thrombin (EC number 3.4.21.5), a microglia-activating agent.
81(1,1,1,1) Details
7577828 Yui N, Suzuki K, Okano T, Sakurai Y, Nakano M, Ishikawa C, Fujimoto K, Kawaguchi H: Cytoplasmic calcium level and membrane fluidity of platelets contacting poly (acrylamide-co-methacrylic acid) particles with different surface properties. J Biomater Sci Polym Ed. 1995;7(3):253-64.

Our previous studies demonstrated interesting features of PAAmMAc particles during interaction with platelets: (1) PAAmMAc particles induce no calcium increase but enhance membrane fluidity of platelets: (2) thrombin induces no calcium increase in platelets when the platelets were mixed previously with PAAmMAc particles; and (3) PAAmMAc particles induce a calcium increase in platelets when they were treated previously with sodium azide (NaN3).
It was also revealed that the suppression of thrombin-induced calcium increase and the membrane fluidity increase in platelets by PAAmMAc particles were reduced by albumin-treatment of the particles.
2(0,0,0,2) Details
9125514 Hunter T, Ikebukuro K, Bannister WH, Bannister JV, Hunter GJ: The conserved residue tyrosine 34 is essential for maximal activity of iron-superoxide dismutase from Escherichia coli. Biochemistry. 1997 Apr 22;36(16):4925-33.

FeSOD and mutant enzymes were purified from SOD-deficient cells using a GST-FeSOD fusion protein intermediate which was subsequently cleaved with thrombin and repurified.
SODY34F exhibited decreased thermal stability, reduced activity at high pH, and a pronounced increase in sensitivity to the inhibitor sodium azide compared with wild-type FeSOD.
1(0,0,0,1) Details
8724599 Khaspekova SG, Vyzova TV, Lukin VV, Tikhomirov OIu, Berndt M, Kouns W, Mazurov AV: [Conformational changes of the platelet membrane glycoprotein IIb-IIIa complex stimulated by a monoclonal antibody to the N-terminal segment of glycoprotein IIIa]. Biokhimiia. 1996 Mar;61(3):412-28.

However, the platelet activation inhibitor, prostaglandin EI, and the mixture of metabolic inhibitors, deoxyglucose-sodium azide, only party inhibited the CRC54-dependent aggregation.
However, the binding of CRC54 increases considerably during activation of platelets by thrombin, platelet adhesion on plastic, GPIIb-IIIa interaction with RGDS-peptide as well as during dissociation of the complex in the presence of EDTA.
1(0,0,0,1) Details
10913362 Hedin HL, Eriksson S, Fowler CJ: Rapid inhibition by sodium azide of the phosphoinositide-mediated calcium response to serotonin stimulation in human platelets: preservation in Alzheimer's disease. Biochem Biophys Res Commun. 2000 Aug 2;274(2):472-6.

A 60 s preincubation with 1 mM NaN (3) reduced the Ca (2+) response to 1 microM serotonin without a corresponding reduction in the responses to 52 mU/ml thrombin or 70 microM beta-amyloid (25-35) (A beta (25-35)).
1(0,0,0,1) Details