Protein Information

Name CD4
Synonyms CD4; CD4 antigen (p55); CD4 molecule; CD4mut; T cell antigen T4/LEU3; T cell surface glycoprotein CD4; T cell surface antigen T4/Leu 3; T cell surface glycoprotein CD4 precursor…

Compound Information

Name sodium azide
CAS sodium azide

Reference List

PubMed Abstract RScore(About this table)
11748622 Lin W, Zhang H, Beck G: Phylogeny of natural cytotoxicity: cytotoxic activity of coelomocytes of the purple sea urchin, Arbacia punctulata. J Exp Zool. 2001 Dec 1;290(7):741-50.

Our immunophenotypic studies showed that A. punctulata phagocytic amebocytes are CD14 (+), CD56 (+), CD158b (+), CD3 (-), CD4 (-), CD8 (-), and CD16 (-).
Sodium azide significantly decreased coelomocyte cytotoxicity, indicating that cytotoxicity is metabolically dependent, and EDTA reduction of cytotoxic activity is consistent with the involvement of divalent cations in the cytotoxic process.
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7790729 Akdis AC, Towbin H, Libsig P, Motz J, Alkan SS: Cytokine immunotrapping: an assay to study the kinetics of production and consumption or degradation of human interferon-gamma. J Immunol Methods. 1995 Jun 9;182(2):251-61.

For production of IFN-gamma, PBMC, purified CD4+ or CD8+ T cells, or diluted whole blood were cultured with different T cell stimulating agents.
Polystyrene macrobeads precoated with an anti-IFN-gamma mAb were put in culture and after a defined incubation period, a dimethyl acridinium ester (DMAE)-labelled second anti-IFN-gamma mAb and sodium azide were added into the culture for additional 24 h.
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9773881 Iyer SB, Hultin LE, Zawadzki JA, Davis KA, Giorgi JV: Quantitation of CD38 expression using QuantiBRITE beads. Cytometry. 1998 Oct 1;33(2):206-12.

The QuantiBRITE bead method was compared with the CD4 biological calibration method for quantitation of CD38 expression on CD8+ T-lymphocytes of Multicenter AIDS Cohort Study participants.
Dilution into either phosphate-buffered saline with 2% fetal calf serum and 0.1% sodium azide or fixation in 1% paraformaldehyde for 1 or 24 h also gave similar results.
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10415028 Fernandes DM, Baird AM, Berg LJ, Rock KL: A monoclonal antibody reactive with a 40-kDa molecule on fetal thymocytes and tumor cells blocks proliferation and stimulates aggregation and apoptosis. J Immunol. 1999 Aug 1;163(3):1306-14.

E710.2.3 is a murine thymic lymphoma cell line with an immature phenotype (CD4-CD8-) that proliferates in response to thymocytes or PMA when cultured at low density and proliferates spontaneously when grown at high density.
The mAb also caused homotypic aggregation of E710.2.3, which was inhibited by cytochalasin B, trifluoperazine, a combination of sodium azide and 2-deoxyglucose, EDTA, incubation at 4 degrees C, or treatment with paraformaldehyde.
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15162424 Zehn D, Cohen CJ, Reiter Y, Walden P: Extended presentation of specific MHC-peptide complexes by mature dendritic cells compared to other types of antigen-presenting cells. Eur J Immunol. 2004 Jun;34(6):1551-60.


Mature human dendritic cells (mDC) were compared with immature DC (iDC), monocytes, CD4 (+) T lymphocytes, melanoma cells, T2 cells and B lymphoblastoid cells for their capacity to present MHC class I-restricted tumor-associated T cell epitopes and were found to display the specific peptides two to six times longer than other cells.
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7677764 Pieters RH, Punt P, Bol M, van Dijken JM, Seinen W, Penninks AH: The thymus atrophy inducing organotin compound DBTC stimulates TcR alpha beta-CD3 signalling in immature rat thymocytes. Biochem Biophys Res Commun. 1995 Sep 14;214(2):552-8.


The responding thymocytes were CD53- and FSClow, thus possibly including the non proliferating counterpart of the presumed immature CD4-CD8+CD53-target cells of DBTC.
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