Protein Information

Name lipase
Synonyms HGL; HPL; HGL; Human giant larvae homolog; LGL 2; LGL2; LLGL 2; LLGL2…

Compound Information

Name sodium azide
CAS sodium azide

Reference List

PubMed Abstract RScore(About this table)
10634941 Brasaemle DL, Levin DM, Adler-Wailes DC, Londos C: The lipolytic stimulation of 3T3-L1 adipocytes promotes the translocation of hormone-sensitive lipase to the surfaces of lipid storage droplets. Biochim Biophys Acta. 2000 Jan 17;1483(2):251-62.

Hormone-sensitive lipase translocation and lipolysis were inhibited by N-ethylmaleimide and a combination of deoxyglucose and sodium azide.
We propose that the major consequence of the phosphorylation of hormone-sensitive lipase following the lipolytic stimulation of adipocytes is the translocation of the lipase from the cytosol to the surfaces of lipid storage droplets.
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12218047 Jongbloed JD, Antelmann H, Hecker M, Nijland R, Bron S, Airaksinen U, Pries F, Quax WJ, van Dijl JM, Braun PG: Selective contribution of the twin-arginine translocation pathway to protein secretion in Bacillus subtilis. J Biol Chem. 2002 Nov 15;277(46):44068-78. Epub 2002 Sep 5.

Sodium azide-inhibition of SecA strongly affected the extracellular appearance of de novo synthesized proteins, including the lipase LipA and two other proteins with predicted RR/KR-signal peptides.
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15995648 Kim DY, Kim HC, Kim SY, Rhee YH: Molecular characterization of extracellular medium-chain-length poly (3-hydroxyalkanoate) depolymerase genes from Pseudomonas alcaligenes strains. J Microbiol. 2005 Jun;43(3):285-94.

The PhaZ (PalLB19) and the PhaZ (PalM4-7) commonly share the lipase box, GISSG, in their catalytic domains, and utilize 111Asn and 110Ser residues, respectively, as oxyanions that play an important role in transition-state stabilization of hydrolytic reactions.
It was insensitive to dithiothreitol, sodium azide, and iodoacetamide, but susceptible to p-hydroxymercuribenzoic acid, N-bromosuccinimide, acetic anhydride, EDTA, diisopropyl fluorophosphate, phenylmethylsulfonyl fluoride, Tween 80, and Triton X-100.
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8130261 Pinson A, Zilberman Y, Tirosh R, Trembovler V, Shohami E: High oligomycin concentrations augment 6-keto-PGF1 alpha production in ventricular cardiomyocytes. Biochim Biophys Acta. 1994 Mar 24;1211(3):283-8.

In cells depleted of ATP, either by low oligomycin concentrations or by sodium azide, 6-keto PGF1 alpha was not appreciably increased.
We suggest that oligomycin at high concentrations causes the release of free arachidonic acid from phospholipids either by non-PIP2-specific PLC and DG lipase or by phospholipase D, phosphatidic acid phosphatase and DG lipase.
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12576071 Lata S, Bhardwaj K, Rajasekharan R: A single-step procedure for the synthesis of photoreactive and radioactive glycerolipids. Anal Biochem. 2003 Feb 1;313(1):155-9.

Iodoazide was generated using [125I] iodomonochloride and sodium azide that was found to add stereospecifically in a variety of olefins.
The synthesized radiolabeled photoprobes were subsequently used to label lipase and phospholipase A (2).
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