Protein Information

Name CD14
Synonyms CD14; PTX 1; PTX1; CDA14; Cd002; ERGIC and golgi 2; ERGIC 2; ERGIC2…

Compound Information

Name sodium azide
CAS sodium azide

Reference List

PubMed Abstract RScore(About this table)
11274165 da Silva Correia J, Soldau K, Christen U, Tobias PS, Ulevitch RJ: Lipopolysaccharide is in close proximity to each of the proteins in its membrane receptor complex. transfer from CD14 to TLR4 and MD-2. J Biol Chem. 2001 Jun 15;276(24):21129-35. Epub 2001 Mar 26.

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11748622 Lin W, Zhang H, Beck G: Phylogeny of natural cytotoxicity: cytotoxic activity of coelomocytes of the purple sea urchin, Arbacia punctulata. J Exp Zool. 2001 Dec 1;290(7):741-50.

Our immunophenotypic studies showed that A. punctulata phagocytic amebocytes are CD14 (+), CD56 (+), CD158b (+), CD3 (-), CD4 (-), CD8 (-), and CD16 (-).
Sodium azide significantly decreased coelomocyte cytotoxicity, indicating that cytotoxicity is metabolically dependent, and EDTA reduction of cytotoxic activity is consistent with the involvement of divalent cations in the cytotoxic process.
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7790729 Akdis AC, Towbin H, Libsig P, Motz J, Alkan SS: Cytokine immunotrapping: an assay to study the kinetics of production and consumption or degradation of human interferon-gamma. J Immunol Methods. 1995 Jun 9;182(2):251-61.

Conversely, addition of monocytes to purified T cells increased the trapping factor suggesting that a substantial amount of IFN-gamma was consumed or degraded both by CD14+ cells as well as T cells in culture.
Polystyrene macrobeads precoated with an anti-IFN-gamma mAb were put in culture and after a defined incubation period, a dimethyl acridinium ester (DMAE)-labelled second anti-IFN-gamma mAb and sodium azide were added into the culture for additional 24 h.
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