Protein Information

Name CD8
Synonyms CD8; p32; MAL; CD8A; CD8a antigen; CD8a molecule; LEU2 T lymphocyte antigen; Leu2…

Compound Information

Name sodium azide
CAS sodium azide

Reference List

PubMed Abstract RScore(About this table)
7790729 Akdis AC, Towbin H, Libsig P, Motz J, Alkan SS: Cytokine immunotrapping: an assay to study the kinetics of production and consumption or degradation of human interferon-gamma. J Immunol Methods. 1995 Jun 9;182(2):251-61.

For production of IFN-gamma, PBMC, purified CD4+ or CD8+ T cells, or diluted whole blood were cultured with different T cell stimulating agents.
Polystyrene macrobeads precoated with an anti-IFN-gamma mAb were put in culture and after a defined incubation period, a dimethyl acridinium ester (DMAE)-labelled second anti-IFN-gamma mAb and sodium azide were added into the culture for additional 24 h.
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11748622 Lin W, Zhang H, Beck G: Phylogeny of natural cytotoxicity: cytotoxic activity of coelomocytes of the purple sea urchin, Arbacia punctulata. J Exp Zool. 2001 Dec 1;290(7):741-50.

Our immunophenotypic studies showed that A. punctulata phagocytic amebocytes are CD14 (+), CD56 (+), CD158b (+), CD3 (-), CD4 (-), CD8 (-), and CD16 (-).
Sodium azide significantly decreased coelomocyte cytotoxicity, indicating that cytotoxicity is metabolically dependent, and EDTA reduction of cytotoxic activity is consistent with the involvement of divalent cations in the cytotoxic process.
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10415028 Fernandes DM, Baird AM, Berg LJ, Rock KL: A monoclonal antibody reactive with a 40-kDa molecule on fetal thymocytes and tumor cells blocks proliferation and stimulates aggregation and apoptosis. J Immunol. 1999 Aug 1;163(3):1306-14.

E710.2.3 is a murine thymic lymphoma cell line with an immature phenotype (CD4-CD8-) that proliferates in response to thymocytes or PMA when cultured at low density and proliferates spontaneously when grown at high density.
The mAb also caused homotypic aggregation of E710.2.3, which was inhibited by cytochalasin B, trifluoperazine, a combination of sodium azide and 2-deoxyglucose, EDTA, incubation at 4 degrees C, or treatment with paraformaldehyde.
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9773881 Iyer SB, Hultin LE, Zawadzki JA, Davis KA, Giorgi JV: Quantitation of CD38 expression using QuantiBRITE beads. Cytometry. 1998 Oct 1;33(2):206-12.

The QuantiBRITE bead method was compared with the CD4 biological calibration method for quantitation of CD38 expression on CD8+ T-lymphocytes of Multicenter AIDS Cohort Study participants.
Dilution into either phosphate-buffered saline with 2% fetal calf serum and 0.1% sodium azide or fixation in 1% paraformaldehyde for 1 or 24 h also gave similar results.
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7898494 Gagnerault MC, Bach JF, Dardenne M, Lepault F: Two different mechanisms for the inhibition of rosette formation in mice. Mol Immunol. 1995 Feb;32(3):177-83.


The vast majority of spleen T cells (T.sRFC) which spontaneously bind to sheep red blood cells (SRBC) in an antigen-specific fashion express the Thy-1+, CD3+, CD8+ phenotype.
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