Protein Information

Name glucagon
Synonyms GCG; GLP 1; GLP1; GLP 2; GLP2; GRPP; Glicentin related polypeptide; Glucagon…

Compound Information

Name sodium azide
CAS sodium azide

Reference List

PubMed Abstract RScore(About this table)
16081632 Olsen HL, Theander S, Bokvist K, Buschard K, Wollheim CB, Gromada J: Glucose stimulates glucagon release in single rat alpha-cells by mechanisms that mirror the stimulus-secretion coupling in beta-cells. Endocrinology. 2005 Nov;146(11):4861-70. Epub 2005 Aug 4.

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9163339 Applegate TL, Karjalainen A, Bygrave FL: Rapid Ca2+ influx induced by the action of dibutylhydroquinone and glucagon in the perfused rat liver. Biochem J. 1997 Apr 15;323 ( Pt 2):463-7.

Administration of sodium azide, an inhibitor of mitochondrial electron transport, also blocks the BHQ plus glucagon-induced Ca2+ influx and this is reversed when azide administration is terminated.
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10572493 Edwin N, Leigh CM: Immunocytochemical identification of islet cells containing calcitonin gene-related peptide-like immunoreactivity in the plains rat pancreas (Pseudomys australis). Singapore Med J. 1999 Aug;40(8):528-30.

The sections were incubated with rabbit polyclonal rat CGRP (Peninsula Labs, Belmont, CA) diluted 1:1000, in a solution consisting of 1% bovine serum albumin and 0.05% sodium azide in 0.1 mol/L of PBST overnight in a humid chamber at room temperature.
RESULTS: For the purpose of colocalization of CGRP immunoreactive cells, mirror images of stained sections were stained for CGRP and insulin, somatostatin, glucagon and pancreatic polypeptide.
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15988591 Misler S, Dickey A, Barnett DW: Maintenance of stimulus-secretion coupling and single beta-cell function in cryopreserved-thawed human islets of Langerhans. Pflugers Arch. 2005 Sep;450(6):395-404. Epub 2005 Jun 30.


Insulin secretion is modulated by blockers of voltage-dependent Na+ and Ca2+ channels, and paracrine hormones (glucagon and somatostatin) in manners indistinguishable from fresh tissue preparations.
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10552906 Ronner P, Friel E, Czerniawski K, Frankle S: Luminometric assays of ATP, phosphocreatine, and creatine for estimation of free ADP and free AMP. Anal Biochem. 1999 Nov 15;275(2):208-16.


We tested the methods with glucagon- and insulin-secreting cells.
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