Protein Information

Name caspase 3
Synonyms Apopain; CASP 3; CASP3; CPP 32; CPP32; CPP32B; Caspase 3; Caspase 3 precursor…

Compound Information

Name mercuric chloride
CAS

Reference List

PubMed Abstract RScore(About this table)
19085830 Zarnescu O: Tracing the accumulation and effects of mercury uptake in the previtellogenic ovary of crucian carp, Carassius auratus gibelio by autometallography and caspase-3 immunohistochemistry. Histol Histopathol. 2009 Feb;24(2):141-8.

The aims of the present study were to apply the AMG technique for localization of mercury at the light and electron microscopic level in the ovary of crucian carp after exposure to mercuric chloride and to find out if this heavy metal induces expression of caspase-3.
8(0,0,1,3) Details
15623433 Maejima Y, Adachi S, Morikawa K, Ito H, Isobe M: Nitric oxide inhibits myocardial apoptosis by preventing caspase-3 activity via S-nitrosylation. J Mol Cell Cardiol. 2005 Jan;38(1):163-74. Epub 2004 Dec 8.

7(0,0,0,7) Details
16764493 Lee JH, Youm JH, Kwon KS: [Mercuric chloride induces apoptosis in MDCK cells] . J Prev Med Public Health. 2006 May;39(3):199-204.

MDCK cells were cultured for 6-24 hr in vitro in various concentrations (0.1-100 M) of HgCl2, and the markers of apoptosis or cell death were assayed, including DNA fragmentation, caspase-3 activity andwestern blotting of cytochrome c.
3(0,0,0,3) Details
12505371 Araragi S, Kondoh M, Kawase M, Saito S, Higashimoto M, Sato M: Mercuric chloride induces apoptosis via a mitochondrial-dependent pathway in human leukemia cells. Toxicology. 2003 Feb 14;184(1):1-9.

In this study, we examined the activation of caspase-3, an important cysteine aspartic protease, during HgCl (2)-induced apoptosis in a human leukemia cell line (HL-60 cells).
3(0,0,0,3) Details
17950395 Laiosa MD, Eckles KG, Langdon M, Rosenspire AJ, McCabe MJ Jr: Exposure to inorganic mercury in vivo attenuates extrinsic apoptotic signaling in Staphylococcal aureus enterotoxin B stimulated T-cells. Toxicol Appl Pharmacol. 2007 Dec 15;225(3):238-50. Epub 2007 Jun 19.

To test this hypothesis, BALB/cJ mice were exposed to 10 mg/l mercuric chloride (HgCl (2)) in their drinking water for 2 weeks followed by injection with 20 microg of the Staphylococcal aureus enterotoxin B (SEB) superantigen.
Eighteen hours after SEB challenge, there was a statistically significant reduction in caspase 8 and caspase 3 enzyme activity in the SEB reactive Vbeta8+ T-cells.
2(0,0,0,2) Details
16273840 Zefferino R, Elia G, Lasalvia M, Piccoli C, Boffoli D, Capitanio N, Ambrosi L: The study of gap junctional intercellular communication in keratinocytes as screening of promoter effect induced by industrial and environmental toxic substances. Med Lav. 2005 May-Jun;96(3):222-30.


The expression of caspase 3, which is the ultimate target to be activated of both mitochondrial- and non-mitochondrial-linked pro-apoptotic pathways, was evaluated using Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR).
2(0,0,0,2) Details
11764948 Pourahmad J, Mihajlovic A, O'Brien PJ: Hepatocyte lysis induced by environmental metal toxins may involve apoptotic death signals initiated by mitochondrial injury. Adv Exp Med Biol. 2001;500:249-52.


Hepatocyte lysis was also prevented by various apoptosis inhibitors eg, cycloheximide, dactinomycin and a tetrapeptide caspase 3 inhibitor which suggests that metal induced hepatocyte lysis involves apoptotic death signals initiated by MPT and "ROS".
1(0,0,0,1) Details
15150681 Toimela T, Tahti H: Mitochondrial viability and apoptosis induced by aluminum, mercuric mercury and methylmercury in cell lines of neural origin. Arch Toxicol. 2004 Oct;78(10):565-74. Epub 2004 May 19.


Apoptosis induced by the metals was measured after 6-, 24- and 48-h exposure times with the determination of activated caspase 3 enzyme.
1(0,0,0,1) Details
9388494 Li J, Billiar TR, Talanian RV, Kim YM: Nitric oxide reversibly inhibits seven members of the caspase family via S-nitrosylation. Biochem Biophys Res Commun. 1997 Nov 17;240(2):419-24.


Caspase-3-like activity was found to be inhibited under these conditions.
1(0,0,0,1) Details
15050407 Kim SH, Sharma RP: Mercury-induced apoptosis and necrosis in murine macrophages: role of calcium-induced reactive oxygen species and p38 mitogen-activated protein kinase signaling. Toxicol Appl Pharmacol. 2004 Apr 1;196(1):47-57.

The cytotoxic EC (50) of mercury ranged from 62.7 to 81.1 microM by various assays in J774A.1 cultures; accordingly, we employed 70 microM of mercuric chloride in most experiments.
Mercury-induced intracellular calcium modulated reactive oxygen species (ROS) production, which resulted in both cell apoptosis and necrosis indicated by annexin V binding and caspase-3 activity, and propidium-iodide binding.
1(0,0,0,1) Details
17363214 Park EJ, Park K: Induction of reactive oxygen species and apoptosis in BEAS-2B cells by mercuric chloride. Toxicol In Vitro. 2007 Aug;21(5):789-94. Epub 2007 Feb 1.

Exposure of cultured cells to mercuric chloride led to cell death, ROS increase, and cytosolic caspase-3 activation.
31(0,1,1,1) Details