| Name | caspase 3 |
|---|---|
| Synonyms | Apopain; CASP 3; CASP3; CPP 32; CPP32; CPP32B; Caspase 3; Caspase 3 precursor… |
| Name | diphenylamine |
|---|---|
| CAS |
| PubMed | Abstract | RScore(About this table) | |
|---|---|---|---|
| 11108134 | von Knethen A, Brune B: Attenuation of macrophage apoptosis by the cAMP-signaling system. Mol Cell Biochem. 2000 Sep;212(1-2):35-43. Selected apoptotic parameters comprised DNA fragmentation (diphenylamine assay), annexin V staining of caspase activity (quantitated by the cleavage of a fluorogenic caspase-3-like substrate Ac-DEVD-AMC), and mitochondrial membrane depolarisation (delta psi). |
81(1,1,1,1) | Details |
| 10602776 | von Knethen A, Brockhaus F, Kleiter I, Brune B: NO-Evoked macrophage apoptosis is attenuated by cAMP-induced gene expression. Mol Med. 1999 Oct;5(10):672-84. Parameters comprised DNA fragmentation (diphenylamine assay), annexin V staining of caspase activity (quantitated by the cleavage of a fluorogenic caspase-3-like substrate Ac-DEVD-AMC), and mitochondrial membrane depolarization (DeltaPsi), analyzed using DiOC (6)(3). |
81(1,1,1,1) | Details |
| 19540331 | Rejiya CS, Cibin TR, Abraham A: Leaves of Cassia tora as a novel cancer therapeutic--an in vitro study. Toxicol In Vitro. 2009 Sep;23(6):1034-8. Epub 2009 Jun 18. Proliferation of HeLa was measured by MTT assay, cell DNA content by modified diphenylamine method and apoptosis by Caspase 3 activity. |
31(0,1,1,1) | Details |
| 15301745 | Zheng XL, Sun HX, Liu XL, Chen YX, Qian BC: Astilbic acid induced COLO 205 cell apoptosis by regulating Bcl-2 and Bax expression and activating caspase-3. Acta Pharmacol Sin. 2004 Aug;25(8):1090-5. Content of DNA in COLO 205 cell was measured by modified diphenylamine assay. |
2(0,0,0,2) | Details |
| 10686588 | Pasquini LA, Besio Moreno M, Adamo AM, Pasquini JM, Soto EF: Lactacystin, a specific inhibitor of the proteasome, induces apoptosis and activates caspase-3 in cultured cerebellar granule cells. J Neurosci Res. 2000 Mar 1;59(5):601-11. The morphological changes produced by the proteasome inhibitor included nuclear condensation and DNA fragmentation measured by the diphenylamine test, as well as a positive labeling by the TUNEL (terminal deoxynucleotidyltransferase mediated- nick end labeling) assay, all of them typical features of apoptosis. |
2(0,0,0,2) | Details |
| 15538571 | Zhang XM, Chen J, Xia YG, Xu Q: Apoptosis of murine melanoma B16-BL6 cells induced by targeting mitochondria, inhibiting expression of PKC-alpha and translocating PKC-delta. Cancer Chemother Pharmacol. 2005 Mar;55(3):251-62. Epub 2004 Nov 5. promoted caspase-3 activity in a dose-dependent manner, which was also regulated by PMA and doxorubicin with a pattern similar to that seen in their effect on apoptosis, mitochondrial membrane potential and Bcl-2 expression, but none of these were directly affected by PMA and doxorubicin. DNA fragmentation was analyzed by electrophoresis and quantified by the diphenylamine method. |
2(0,0,0,2) | Details |
| 19705349 | Abid-Essefi S, Bouaziz C, Golli-Bennour EE, Ouanes Z, Bacha H: Comparative study of toxic effects of zearalenone and its two major metabolites alpha-zearalenol and beta-zearalenol on cultured human Caco-2 cells. J Biochem Mol Toxicol. 2009 Jul;23(4):233-43. To check whether the oxidative stress induction was associated to DNA lesions, we looked for DNA fragmentation by means of the Comet and the diphenylamine assays. To specify cell death pathway, we investigated caspase-3 activation, confirmed by poly (ADP- polymerase cleavage and by Bcl-2 depletion. |
2(0,0,0,2) | Details |
| 19168702 | Noe J, Petrusca D, Rush N, Deng P, VanDemark M, Berdyshev E, Gu Y, Smith P, Schweitzer K, Pilewsky J, Natarajan V, Xu Z, Obukhov AG, Petrache I: CFTR regulation of intracellular pH and ceramides is required for lung endothelial cell apoptosis. Am J Respir Cell Mol Biol. 2009 Sep;41(3):314-23. Epub 2009 Jan 23. Both specific CFTR inhibition with 2-(phenylamino) diphenylamine-2-carboxylic acid, 5-[(4-carboxyphenyl) methylene]-2-thioxo-3-[(3-trifluoromethyl) phenyl-4-thi azolidinone (CFTR (inh)-172), or 5-nitro-2-(3-phenylpropylamino) and CFTR knockdown significantly attenuated endothelial cell apoptosis induced by staurosporine or H (2) O (2). TUNEL staining and active caspase-3 immunohistochemistry indicated that cellular apoptosis was decreased in lung explants from patients with cystic fibrosis compared with those with smoking-induced chronic obstructive lung disease, especially in the alveolar tissue and vascular endothelium. |
1(0,0,0,1) | Details |
| 10780973 | Messmer UK, Winkel G, Briner VA, Pfeilschifter J: Suppression of apoptosis by glucocorticoids in glomerular endothelial cells: effects on proapoptotic pathways. Br J Pharmacol. 2000 Apr;129(8):1673-83. Tumour necrosis factor-alpha (TNF-alpha)- and lipopolysaccharide (LPS)-induced apoptosis of bovine glomerular endothelial cells is now recognized as an important part in the pathogenesis of glomerulonephritis characterized by early mitochondrial cytochrome c release, mitochondrial permeability transition, Bak protein upregulation, Bcl-X (L) protein downregulation and caspase-3 activation. The action of glucocorticoids could be documented in that they prevented all apoptotic markers such as DNA laddering, DNA fragmentation measured by the diphenylamine assay as well as morphological alterations. |
2(0,0,0,2) | Details |