| 14734216 |
Takagi K, Tatsumi Y, Kitaichi K, Iwase M, Shibata E, Nakao M, Matsumoto T, Takagi K, Hasegawa T: A sensitive colorimetric assay for polyamines in erythrocytes using oat seedling polyamine oxidase. Clin Chim Acta. 2004 Feb;340(1-2):219-27.
In the presence of the H (2) O (2) produced by this polyamine oxidase reaction and a new chromogen, N-(carboxymethylaminocarbonyl)-4,4'-bis (dimethylamino)-diphenylamine sodium salt (DA-64), peroxidase (POD) catalyzes the formation of N-[4-[[4-(dimethylamino) phenyl] imino]-2,5-cyclohexadien-1-ylidene]-N-methy lmethanaminium chloride (Bindschedler's Green) having an absorption maximum at 727 nm. |
33(0,1,1,3) |
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