Protein Information

Name annexin V
Synonyms ANX 5; PP4; ENX2; ANX5; ANXA 5; ANXA5; Anchorin CII; Annexin 5…

Compound Information

Name sodium arsenite
CAS sodium arsenenite

Reference List

PubMed Abstract RScore(About this table)
9751585 DeMeester SL, Qiu Y, Buchman TG, Hotchkiss RS, Dunnigan K, Karl IE, Cobb JP: Nitric oxide inhibits stress-induced endothelial cell apoptosis. Crit Care Med. 1998 Sep;26(9):1500-9.

MEASUREMENTS AND MAIN RESULTS: The sequence LPS/sodium arsenite increased the rate of endothelial cell apoptosis (47.4%, p < .05 vs. control), as measured by fluorescent-activated cell scanning using annexin V/propidium iodide staining.
81(1,1,1,1) Details
12208374 Huang HS, Chang WC, Chen CJ: Involvement of reactive oxygen species in arsenite-induced downregulation of phospholipid hydroperoxide glutathione peroxidase in human epidermoid carcinoma A431 cells. Free Radic Biol Med. 2002 Sep 15;33(6):864-73.

With the aid of agarose gel electrophoresis, and propidium iodide and annexin-V staining, we found that treatment of 30 microM sodium arsenite for 24 h induced apoptosis in human epidermoid carcinoma A431 cells and EA.hy926 cells.
6(0,0,1,1) Details
16384727 Brink A, Schulz B, Kobras K, Lutz WK, Stopper H: Time-dependent effects of sodium arsenite on DNA breakage and apoptosis observed in the comet assay. Mutat Res. 2006 Feb 28;603(2):121-8. Epub 2005 Dec 27.

In parallel, we evaluated apoptosis by measuring annexin V-positive cells with flow cytometry, and visualized apoptotic cells on slides by Hoechst bisbenzimide 33258 staining.
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9706160 DeMeester SL, Cobb JP, Hotchkiss RS, Osborne DF, Karl IE, Tinsley KW, Buchman TG: Stress-induced fractal rearrangement of the endothelial cell cytoskeleton causes apoptosis. Surgery. 1998 Aug;124(2):362-71.

METHODS: Porcine aortic endothelial cells in culture, surrogates for the microvasculature in vivo, were exposed sequentially to Escherichia coli endotoxin (25 micrograms/mL; 18 hours) to induce the inflammatory response and then to sodium arsenite (160 mumol/L; 120 minutes) to induce the heat-shock response, a well-characterized model of stress-induced apoptosis.
Other cells were exposed to cytochalasin D, a fungal metabolite, which interferes with polymerization of actin from its globular to its filamentous form, and similarly were analyzed with respect to fractal dimension, viability (neutral red assay), and manner of death (annexin V fluorescence-activated cell scanning analysis).
1(0,0,0,1) Details
10507293 Van Oostveldt K, Dosogne H, Burvenich C, Paape MJ, Brochez V, Van den Eeckhout E: Flow cytometric procedure to detect apoptosis of bovine polymorphonuclear leukocytes in blood. Vet Immunol Immunopathol. 1999 Sep 1;70(1-2):125-33.

A flow cytometric technique was used to detect apoptosis and necrosis of bovine polymorphonuclear neutrophil leukocytes (PMN) using fluorescein isothiocyanate labeled annexin-V and propidium iodide (PI).
To establish a positive control for PMN apoptosis, the effect of cycloheximide, actinomycin D, diamide, buthionine sulfoximine and sodium arsenite, that have been described to induce apoptosis by various mechanisms was tested.
1(0,0,0,1) Details
9288129 Jacob AK, Hotchkiss RS, DeMeester SL, Hiramatsu M, Karl IE, Swanson PE, Cobb JP, Buchman TG: Endothelial cell apoptosis is accelerated by inorganic iron and heat via an oxygen radical dependent mechanism. Surgery. 1997 Aug;122(2):243-53; discussion 254.

METHODS: HUVECs were loaded with Fe [III](ferric chloride and ferric ammonium citrate) with 8-hydroxyquinoline as carrier and were then challenged with two stimuli of the heat shock response, authentic heat or sodium arsenite.
The mechanism of cell death was assessed with three complementary techniques, Annexin V/propidium iodide labeling, the TUNEL stain, and electron microscopy.
1(0,0,0,1) Details
9403531 DeMeester SL, Buchman TG, Qiu Y, Jacob AK, Dunnigan K, Hotchkiss RS, Karl I, Cobb JP: Heat shock induces IkappaB-alpha and prevents stress-induced endothelial cell apoptosis. Arch Surg. 1997 Dec;132(12):1283-7; discussion 1287-8.

INTERVENTIONS: Cultured primary porcine endothelial cells were treated with increasing doses of sodium arsenite (40-160 micromol/L), after which the interval until subsequent apoptotic (lipopolysaccharide-arsenite) challenge was varied (4-16 hours).
The degree of cell death and apoptosis were determined using neutral red uptake and staining with annexin V and propidium iodide, respectively.
1(0,0,0,1) Details