| Name | ScFvs |
|---|---|
| Synonyms | SCFV; Single chain Fv; scFv; single chain Fv fragment; Single chain Fvs; scFvs; single chain Fv fragments |
| Name | atrazine |
|---|---|
| CAS |
| PubMed | Abstract | RScore(About this table) | |
|---|---|---|---|
| 12487314 | Kramer K: Evolutionary affinity and selectivity optimization of a pesticide-selective antibody utlizing a hapten-selective immunoglobulin repertoire. Environ Sci Technol. 2002 Nov 15;36(22):4892-8. Improved scFv variants were selected by phage display employing an atrazine derivative. |
69(0,2,3,4) | Details |
| 14596835 | Kim JY, Mulchandani A, Chen W: An immunoassay for atrazine using tunable immunosorbent. Anal Biochem. 2003 Nov 15;322(2):251-6. Tunable immunosorbents consisting of a fusion between an elastin-like polypeptide made up of 77 repeating units of the pentapeptide VPGVG and a single-chain Fv of an anti-atrazine antibody were synthesized biologically and purified by temperature-triggered phase transition. |
31(0,1,1,1) | Details |
| 10347888 | Dhillon JK, Drew PD, Porter AJ: Bacterial surface display of an anti-pollutant antibody fragment. . Lett Appl Microbiol. 1999 May;28(5):350-4. A peptidoglycan-associated lipoprotein (PAL) fused to an antibody fragment (scFv) specific to the herbicide and environmental pollutant atrazine, has been successfully targeted to the cell surface of Escherichia coli. |
31(0,1,1,1) | Details |
| 10563896 | Grant SD, Porter AJ, Harris WJ: Comparative sensitivity of immunoassays for haptens using monomeric and dimeric antibody fragments. J Agric Food Chem. 1999 Jan;47(1):340-5. A single-chain anti-atrazine antibody fragment, scAb (single-chain Fv with a CK domain), was expressed in Escherichia coli, and monomeric and dimeric species were preferentially purified from periplasmic extracts by chromatography upon chelate immunosorbent columns or by immunoaffinity purification using a constant domain (CK) tag. |
31(0,1,1,1) | Details |
| 9685621 | Longstaff M, Newell CA, Boonstra B, Strachan G, Learmonth D, Harris WJ, Porter AJ, Hamilton WD: Expression and characterisation of single-chain antibody fragments produced in transgenic plants against the organic herbicides atrazine and paraquat. Biochim Biophys Acta. 1998 Jul 23;1381(2):147-60. Single-chain antibody fragments (scAbs), which have a human C-kappa constant domain and a hexa- tail attached to the carboxy terminus of the single-chain Fv (ScFv) fragments to facilitate purification, have been raised against the herbicides paraquat and atrazine and expressed in transgenic Nicotiana tabacum cv. |
31(0,1,1,1) | Details |
| 12655454 | Oelschlaeger P, Lange S, Schmitt J, Siemann M, Reuss M, Schmid RD: Identification of factors impeding the production of a single-chain antibody fragment in Escherichia coliby comparing in vivo and in vitro expression. Appl Microbiol Biotechnol. 2003 Apr;61(2):123-32. Epub 2003 Jan 28. The atrazine-specific single-chain variable antibody fragments (scFv) K411B was produced by expression in either the cytoplasm or the periplasm of Escherichia coli BL21 (DE3). |
9(0,0,1,4) | Details |
| 12033639 | Rau D, Kramer K, Hock B: Single-chain Fv antibody-alkaline phosphatase fusion proteins produced by one-step cloning as rapid detection tools for ELISA. J Immunoassay Immunochem. 2002;23(2):129-43. |
7(0,0,0,7) | Details |
| 10648163 | Hayhurst A: Improved expression characteristics of single-chain Fv fragments when fused downstream of the Escherichia coli -binding protein or upstream of a single immunoglobulin-constant domain. Protein Expr Purif. 2000 Feb;18(1):1-10. By fusing the E. coli -binding protein (mbp) gene either 3' or 5' to a scAb specific for the herbicide atrazine, a reduction in growth arrest was observed that was dependent on the order of gene fusion. |
6(0,0,0,6) | Details |
| 10679216 | Li Y, Cockburn W, Kilpatrick JB, Whitelam GC: High affinity ScFvs from a single rabbit immunized with multiple haptens. Biochem Biophys Res Commun. 2000 Feb 16;268(2):398-404. The rabbit was immunised with a mixture of protein conjugates of four different haptens, namely the herbicide mecoprop and derivatives of the herbicides atrazine, simazine, and isoproturon. |
5(0,0,0,5) | Details |
| 11936096 | Rau D, Kramer K, Hock B: Cloning, functional expression and kinetic characterization of pesticide-selective Fab fragment variants derived by molecular evolution of variable antibody genes. Anal Bioanal Chem. 2002 Jan;372(2):261-7. Epub 2001 Dec 22. The affinity alteration was also reflected in the 22-fold reduction of the IC50 values of the variants Fab IPR-7 (IC50 = 60.5 microg/L) and Fab IPR-23 (IC50=2.7 microg/L) in the corresponding atrazine ELISA. Fab antibody fragments were constructed by subcloning single chain Fv variable regions from the phagemid vector pCANTAB 5E into the expression vector pASK99. |
3(0,0,0,3) | Details |