Protein Information

Name alkaline phosphatase
Synonyms ALP 1; Alkaline phosphatase; ALPG; ALPPL; ALPPL 2; ALPPL2; GCAP; Germ cell alkaline phosphatase…

Compound Information

Name atrazine
CAS

Reference List

PubMed Abstract RScore(About this table)
8309947 Ward VK, Schneider PG, Kreissig SB, Hammock BD, Choudary PV: Cloning, sequencing and expression of the Fab fragment of a monoclonal antibody to the herbicide atrazine. Protein Eng. 1993 Nov;6(8):981-8.

The expressed products were confirmed as atrazine reactive by binding to an atrazine derivative conjugated with alkaline phosphatase.
81(1,1,1,1) Details
10347888 Dhillon JK, Drew PD, Porter AJ: Bacterial surface display of an anti-pollutant antibody fragment. . Lett Appl Microbiol. 1999 May;28(5):350-4.

Anti-atrazine binding could be observed via an atrazine-alkaline phosphatase conjugate.
81(1,1,1,1) Details
10563896 Grant SD, Porter AJ, Harris WJ: Comparative sensitivity of immunoassays for haptens using monomeric and dimeric antibody fragments. J Agric Food Chem. 1999 Jan;47(1):340-5.

Recombinant monomeric and dimeric antibody fragments, Fab, and intact monoclonal antibodies were compared in assays by competition between free atrazine in solution and (a) immobilized atrazine-bovine serum albumin conjugate (indirect assay) or (b) atrazine-alkaline phosphatase (direct assay).
31(0,1,1,1) Details
9519450 Vianello F, Signor L, Pizzariello A, Di Paolo ML, Scarpa M, Hock B, Giersch T, Rigo A: Continuous flow immunosensor for atrazine detection. Biosens Bioelectron. 1998 Jan 1;13(1):45-53.

The hapten atrazine was detected under continuous flow conditions using a micro-column which contained immobilized monoclonal antibodies (Ab) against atrazine and atrazine labeled with alkaline phosphatase (An*).
6(0,0,1,1) Details
12033639 Rau D, Kramer K, Hock B: Single-chain Fv antibody-alkaline phosphatase fusion proteins produced by one-step cloning as rapid detection tools for ELISA. J Immunoassay Immunochem. 2002;23(2):129-43.

2(0,0,0,2) Details
16786390 Lopez L, Pozo C, Rodelas B, Calvo C, Gonzalez-Lopez J: Influence of pesticides and herbicides presence on phosphatase activity and selected bacterial microbiota of a natural lake system. Ecotoxicology. 2006 Jul;15(5):487-93.

Our data show that dissolved alkaline phosphatase was the enzymatic activity that contributed in higher proportion to total lake water samples phosphatase status.
The presence of organochlorinated insecticides (aldrin and lindane), organophosphorous insecticides (dimetoate, methidation and methyl-parathion), herbicide atrazine and fungicide captan significantly increased phosphatase activities after 28 days of incubation.
1(0,0,0,1) Details
7682501 Neskovic NK, Elezovic I, Karan V, Poleksic V, Budimir M: Acute and subacute toxicity of atrazine to carp (Cyprinus carpio L.). Ecotoxicol Environ Saf. 1993 Apr;25(2):173-82.

Biochemical changes were most prominent in the alkaline phosphatase, glutamic-oxaloacetic transaminase, and glutamic-pyruvic transaminase activities whereas the most severe histopathological changes were observed in the gills.
1(0,0,0,1) Details
7560745 Gojmerac T, Kartal B, Zuric M, Curic S, Mitak M: Serum biochemical and histopathological changes related to the hepatic function in pigs following atrazine treatment. J Appl Toxicol. 1995 May-Jun;15(3):233-6.

Serum activities of gamma-glutamyltransferase (GGT), alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (AP) were determined.
1(0,0,0,1) Details
14502587 Mosleh YY, Ismail SM, Ahmed MT, Ahmed YM: Comparative toxicity and biochemical responses of certain pesticides to the mature earthworm Aporrectodea caliginosa under laboratory conditions. Environ Toxicol. 2003 Oct;18(5):338-46.

This study was conducted to investigate the toxicity of aldicarb, cypermethrin, profenofos, chlorfluazuron, atrazine, and metalaxyl toward mature Aporrectodea caliginosa earthworms.
The effects of the LC (25) values of these pesticides on the growth rate in relation to glucose, soluble protein, and activities of glutamic-oxaloacetic transaminase (GOT), glutamic-pyruvic transaminase (GPT), acid phosphatase (AcP), and alkaline phosphatase (AIP) were also studied.
1(0,0,0,1) Details
10552555 Wittmann C, Schreiter PY: Analysis of terbuthylazine in soil samples by two test strip immunoassay formats using reflectance and luminescence detection. J Agric Food Chem. 1999 Jul;47(7):2733-7.

The measuring range for the dipstick IA (using an alkaline phosphatase tracer, a 5-bromo-4-chloroindolylphosphate/nitro blue tetrazolium substrate and a portable reflectometer) was from 3 to 300 microg/kg.
From 24 soil samples collected in the Veneto area, Italy, 18 samples contained different amounts of terbuthylazine (but <1 microg/kg atrazine) as was analyzed by gas chromatography with mass selective detection.
1(0,0,0,1) Details
8507100 Chaturvedi AK: Biochemical and toxicological studies on the mixtures of three commonly-used herbicides in mice. Arch Environ Contam Toxicol. 1993 May;24(4):449-54.

Alachlor (AL), atrazine (AT), picloram (PI), AL+AT, AT+PI, PI+AL and AL+AT+PI, at 10 ppm (in drinking water) of each herbicide, were provided to mice for 30, as well as for 90, days ad libitum; these herbicides and mixtures, at 100 mg/kg (in corn oil) of each herbicide, were also given to additional groups of mice by oral intubation daily for 21 days.
Serum glutamic-pyruvic transaminase (SGPT), glutamic-oxalacetic transaminase (SGOT) and alkaline phosphatase activities with AL+AT+PI were elevated by 36-92% on Day 91; SGPT (34%) and SGOT (73%) activities were increased with AL.
1(0,0,0,1) Details