Protein Information

Name diaphorase
Synonyms Diaphorase; DHQU; DIA 4; DIA4; DT diaphorase; DTD; Diaphorase (NADH/NADPH); Diaphorase (NADH/NADPH) (cytochrome b 5 reductase)…

Compound Information

Name chloralose
CAS

Reference List

PubMed Abstract RScore(About this table)
8986872 Krowicki ZK, Sharkey KA, Serron SC, Nathan NA, Hornby PJ: Distribution of nitric oxide synthase in rat dorsal vagal complex and effects of microinjection of nitric oxide compounds upon gastric motor function. J Comp Neurol. 1997 Jan 6;377(1):49-69.

To determine a role of NO in the DVC, NO-related agents were microinjected into the DVC in alpha-chloralose-anesthetized rats while recording indices of gastric motor function.
The location of NADPH-diaphorase-positive staining (a marker of NOS activity) and NOS immunoreactivity overlap in the DVC.
3(0,0,0,3) Details
11788407 Li YF, Roy SK, Channon KM, Zucker IH, Patel KP: Effect of in vivo gene transfer of nNOS in the PVN on renal nerve discharge in rats. Am J Physiol Heart Circ Physiol. 2002 Feb;282(2):H594-601.

In alpha-chloralose- and urethane-anesthetized rats, microinjection of N (G)-monomethyl-L-arginine (L-NMMA), a NO antagonist, into the PVN produced a dose-dependent increase in RSND, blood pressure, and heart rate.
In Ad.nNOS-treated rats, the local expression of nNOS within the PVN was confirmed by histochemistry for NADPH-diaphorase-positive neurons.
2(0,0,0,2) Details
15732761 Maznychenko AV: [Effect of muscle pain on c-fos expression and NADPH-diaphorase activity in the spinal cord]. Fiziol Zh. 2004;50(6):62-75.

Capsaicin (Sigma, 5 mg/l ml) was unilaterally injected into dorsal neck muscles (mm. trapezius, splenius) of the cat (n = 6) anesthetized with alpha-chloralose.
1(0,0,0,1) Details
16322352 Zheng H, Mayhan WG, Bidasee KR, Patel KP: Blunted nitric oxide-mediated inhibition of sympathetic nerve activity within the paraventricular nucleus in diabetic rats. Am J Physiol Regul Integr Comp Physiol. 2006 Apr;290(4):R992-R1002. Epub 2005 Dec 1.


In alpha-chloralose- and urethane-anesthetized rats, an inhibitor of NO synthase, l-NMMA, microinjected into the PVN produced a dose-dependent increase in RSNA, mean arterial pressure (MAP), and heart rate (HR) in both control and diabetic rats.
0(0,0,0,0) Details
16168409 Pilyavskii AI, Maznychenko AV, Maisky VA, Kostyukov AI, Hellstrom F, Windhorst U: Capsaicin-induced effects on c-fos expression and NADPH-diaphorase activity in the feline spinal cord. Eur J Pharmacol. 2005 Oct 3;521(1-3):70-8. Epub 2005 Sep 15.

The distribution of c-fos expression and NADPH-diaphorase reactivity in the cervical and lumbar segments after stimulation of the vanilloid receptors in the dorsal neck muscles with capsaicin was studied in cats anaesthetized with alpha-chloralose.
33(0,1,1,3) Details
11514264 Zhang K, Li YF, Patel KP: Blunted nitric oxide-mediated inhibition of renal nerve discharge within PVN of rats with heart failure. Am J Physiol Heart Circ Physiol. 2001 Sep;281(3):H995-1004.

Subsequently, we have demonstrated that NADPH diaphorase (a commonly used marker for nNOS activity) positive neurons are decreased in paraventricular nucleus (PVN) of rats with coronary artery ligation model of HF.
In alpha-chloralose- and urethane-anesthetized rats, an inhibitor of NO synthase, N (G)-monomethyl-L-arginine (L-NMMA) microinjected into the PVN (50, 100, and 200 pmol in 50-200 nl) produced a dose-dependent increase in RSND, blood pressure, and heart rate in control and HF rats.
1(0,0,0,1) Details