| Name | polynucleotide kinase |
|---|---|
| Synonyms | Bifunctional polynucleotide phosphatase/kinase; PNK; DNA 5' kinase/3' phosphatase; DNA kinase; PNKP; PNKP protein; Polynucleotide kinase; Polynucleotide kinase 3 prime phosphatase… |
| Name | acrolein |
|---|---|
| CAS | 2-propenal |
| PubMed | Abstract | RScore(About this table) | |
|---|---|---|---|
| 3370625 | Smith RA, Sysel IA, Tibbels TS, Cohen SM: Implications for the formation of abasic sites following modification of polydeoxycytidylic acid by acrolein in vitro. Cancer Lett. 1988 May;40(1):103-9. These data show that acrolein-modified 3'-monophosphates are substrates for 32P labeling by T4 polynucleotide kinase and are stable under the assay conditions employed. |
82(1,1,1,2) | Details |
| 2334905 | Smith RA, Williamson DS, Cerny RL, Cohen SM: Detection of 1,N6-propanodeoxyadenosine in acrolein-modified polydeoxyadenylic acid and DNA by 32P postlabeling. Cancer Res. 1990 May 15;50(10):3005-12. In preliminary experiments, polydeoxyadenylic acid was incubated with excess acrolein and then digested to 3' monophosphates prior to transfer of 32P from [gamma-32P] ATP with T4 polynucleotide kinase. |
81(1,1,1,1) | Details |
| 2519782 | Smith RA, Williamson DS, Cohen SM: Identification of 3,N4-propanodeoxycytidine 5'-monophosphate formed by the reaction of acrolein with 5'-monophosphate. Chem Res Toxicol. 1989 Jul-Aug;2(4):267-71. In order to address the utility of 32P postlabeling for the detection of this exocyclic adduct in acrolein-modified nucleic acids, an acrolein- 3'-monophosphate reaction mixture was subjected to 32P postlabeling. 3-Dephosphorylation with nuclease P1 and the 3'-phosphatase activity of T4 polynucleotide kinase yields a nucleotide 5'-[32P] monophosphate which cochromatographs with 3,N4-propanodeoxycytidine 5'-monophosphate. |
81(1,1,1,1) | Details |
| 1394614 | Maccubbin AE, Lee L, Struck RF, Gurtoo HL: 32P-postlabeling of acrolein- adducts in DNA after nuclease P1 digestion. Chem Biol Interact. 1992 Sep 14;84(1):21-35. As nucleotides, the adducts were good substrates for polynucleotide kinase-mediated transfer of from ATP and were able to be detected by 32P-postlabeling. |
1(0,0,0,1) | Details |