Protein Information

Name beta glucuronidase
Synonyms Beta glucuronidase; Beta G1; Beta glucuronidase precursor; GUSB; MPS 7; MPS7; Beta G1s; Beta glucuronidase precursors

Compound Information

Name methylene chloride
CAS dichloromethane

Reference List

PubMed Abstract RScore(About this table)
1293402 Joern WA: Confirmation of low concentrations of urinary benzodiazepines, including alprazolam and triazolam, by GC/MS: an extractive alkylation procedure. J Anal Toxicol. 1992 Nov-Dec;16(6):363-7.

Urine samples containing diazolo- and triazolobenzodiazepines and metabolites were hydrolyzed with beta-glucuronidase and extracted with methylene chloride.
81(1,1,1,1) Details
3571429 Shilo L, Werber MM, Dolev S, Shapiro M, Shenkman L: Digoxin-like immunoreactivity: occurrence in three molecular forms and partial characterization. J Clin Endocrinol Metab. 1987 Jun;64(6):1257-60.

Using gel filtration chromatography, extraction with methylene chloride, and treatment with beta-glucuronidase, we determined that serum DLI exists in three forms: protein-bound, glucuronidated, and free, with respective mol wt of 5000, 400, and 230.
31(0,1,1,1) Details
1286289 Sarkar MA, March C, Karnes HT: Solid phase extraction and simultaneous high performance liquid chromatographic determination of antipyrine and its major metabolites in urine. Biomed Chromatogr. 1992 Nov-Dec;6(6):300-4.

A reversed phase gradient high performance liquid chromatographic method utilizing solid phase extraction has been described for the simultaneous determination of antipyrine (AP), 4-hydroxyantipyrine (4-OHAP), norantipyrine (NorAP) and 3-hydroxymethylantipyrine (3-OHMAP) in human urine after hydrolysis with beta-glucuronidase.
The C-18 sorbent cartridges were conditioned and urine samples were applied, washed with 1 x 4 mL of phosphate buffer and eluted with 3 x 100 microL of 20% v/v of acetonitrile in methylene chloride.
1(0,0,0,1) Details
3050496 Doolittle DJ, Lee DA, Rahn CA: The use of rat urine extracted on XAD-2 resin and assayed in a microsuspension-modified Ames test as an in vivo indicator of genotoxic exposure. Mutat Res. 1988 Oct;206(2):141-8.


In both AAF- and BP-treated animals, extraction of the urine on XAD-2 resin markedly enhanced the mutagenic response compared to neat urine, but partitioning of the XAD-2 eluate into methylene chloride always diminished the mutagenicity of the urine extract.
0(0,0,0,0) Details
2977128 Scott PM, Lawrence GA: Liquid chromatographic determination of zearalenone and alpha- and beta-zearalenols in milk. J Assoc Off Anal Chem. 1988 Nov-Dec;71(6):1176-9.

Essentially, the method includes extraction with basic acetonitrile, acidification, partition into methylene chloride on a hydrophilic matrix, cleanup on an aminopropyl solid phase extraction column, and reverse-phase liquid chromatography with fluorescence detection.
Enzymes (beta-glucuronidase and aryl sulfatase) may be added to milk prior to extraction to hydrolyze any conjugates.
1(0,0,0,1) Details