Protein Information

Name alpha toxin
Synonyms CBP; CBP; Csk binding protein; PAG; PAG 1; PAG1; Phosphoprotein associated with glycosphingolipid enriched microdomains; Phosphoprotein associated with glycosphingolipid enriched microdomains 1…

Compound Information

Name boric acid
CAS

Reference List

PubMed Abstract RScore(About this table)
330401 Takeda Y, Ogiso Y, Miwatani T: Effect of zinc ion on the hemolytic activity of thermostable direct hemolysin from Vibrio parahaemolyticus, streptolysin O, and Triton X-100. Infect Immun. 1977 Aug;17(2):239-43.

We found that in certain buffers, such as tris (hydroxymethyl)-aminomethane-hydrochloride, boric acid-borax, and N-hydroxyethyl piperazine-N'-2-ethanesulfonic acid-sodium hydroxide, hemoglobins released from erythrocytes were easily precipitated by addition of Zn2+, thus resulting in a false inhibition of hemolysin by Zn2+ when hemolysis was assayed by measuring absorbance at 540 nm of released hemoglobins.
83(1,1,1,3) Details
3689801 Hebert TE, Fackrell HB: Inhibition of staphylococcal alpha-toxin by covalent modification of an arginine residue. Biochim Biophys Acta. 1987 Dec 18;916(3):419-27.

Trypsin digestion of modified alpha-toxin generated a 20 kDa fragment which was isolated using a boric acid gel column.
34(0,1,1,4) Details
6156289 Kravchuk GP: [Use of boric acid for increasing the sensitivity of the hemolysin detection test] Lab Delo. 1980;(5):306-8.
6(0,0,1,1) Details
3448599 Krolak M, Blaszczyk B: [Standardization of the complement fixation test (CFT) in brucellosis. Pol Arch Weter. 1986;25(1):33-48.

Lyophilized complement (C) was dissolved, conserved with boric acid +K2SO4 containing liquid and used after 5 days of stabilization.
Lyophilized hemolysin was dissolved, conserved with glycerol and used after 5 days of stabilization.
3(0,0,0,3) Details