Protein Information

Name transferase
Synonyms 4' phosphopantetheinyl transferase; 4' phosphopantetheinyl transferase; AASD PPT; AASDHPPT; AASDPPT; Alpha aminoadipic semialdehyde dehydrogenase phosphopantetheinyl transferase; Aminoadipate semialdehyde dehydrogenase phosphopantetheinyl transferase; CGI 80…

Compound Information

Name boric acid
CAS

Reference List

PubMed Abstract RScore(About this table)
4003757 Smithers GW, O'Sullivan WJ: Hypoxanthine phosphoribosyltransferase: radiochemical assay procedures for the forward and reverse reactions. Anal Biochem. 1985 Feb 15;145(1):14-20.

Enzyme activity in the reverse direction was assessed by measuring the amount of [8-14C] uric acid formed from the labeled IMP following their separation by polyethyleneimine-cellulose TLC in 0.2 M LiCl saturated with boric acid (pH 4.5):95% ethanol (1:1, v/v), the transferase reaction being coupled with excess xanthine oxidase and catalase to overcome the unfavorable equilibrium.
31(0,1,1,1) Details
18176783 Scorei R, Ciubar R, Ciofrangeanu CM, Mitran V, Cimpean A, Iordachescu D: Comparative effects of boric acid and calcium fructoborate on breast cancer cells. Biol Trace Elem Res. 2008 Jun;122(3):197-205. Epub 2008 Jan 5.

This was also demonstrated by terminal deoxynucleotidyl transferase-mediated 2'-deoxyuridine 5'-triphosphate nick-end-labeling assay.
1(0,0,0,1) Details
7251775 Uchikura K, Horikawa R, Tanimura T, Kabasawa Y: Determination of catecholamines by radioenzymatic assay using ion-pair liquid chromatography. J Chromatogr. 1981 Apr 10;223(1):41-50.

A simple radioenzymatic method for the simultaneous determination of norepinephrine, epinephrine and dopamine in human serum was developed. catecholamines were converted to normetanephrine (NMN), metanephrine (MN), 3-methoxytyramine (3-MT) and their 4-O-methyl isomers by catechol O-methyl transferase with S-adenosylmethionine-(3H-methyl) ([3H] SAM).
After addition of unlabelled NMN, MN and 3-MT as carriers, the methyl derivatives were isolated from the reaction mixture by passing through a small boric acid gel column and separated from each other by ion-pair liquid chromatography.
1(0,0,0,1) Details
12243836 Kinashi Y, Masunaga S, Ono K: Mutagenic effect of borocaptate sodium and boronophenylalanine in neutron capture therapy. Int J Radiat Oncol Biol Phys. 2002 Oct 1;54(2):562-7.

PURPOSE: To investigate the mutagenic effect in boron neutron capture therapy (BNCT), Chinese hamster ovary cells were incubated with 10 B-enriched borocaptate sodium (BSH) or para-boronophenylalanine (BPA) before exposure to thermal neutrons, and the occurrence of mutations at the hypoxanthine-guanine phosphoribosyl transferase (HPRT) locus was measured.
RESULTS: The mutagenicity of BSH and BPA was similar to that of 10B boric acid when the cells were irradiated with neutrons at an isosurvival dose after 2-h preincubation.
1(0,0,0,1) Details