Protein Information

Name lipase
Synonyms HGL; HPL; HGL; Human giant larvae homolog; LGL 2; LGL2; LLGL 2; LLGL2…

Compound Information

Name diatomaceous earth
CAS diatomite

Reference List

PubMed Abstract RScore(About this table)
18850711 Lumor SE, Akoh CC: Esterification and hydrolytic activities of Candida rugosa lipase isoform 1 (LIP1) immobilized on celite 545, duolite A7, and sephadex G-25. J Agric Food Chem. 2008 Nov 12;56(21):10396-8. Epub 2008 Oct 14.

2(0,0,0,2) Details
18437590 Sagiroglu A: Conversion of sunflower oil to biodiesel by alcoholysis using immobilized lipase. Artif Cells Blood Substit Immobil Biotechnol. 2008;36(2):138-49.

2(0,0,0,2) Details
15195711 Sagiroglu A, Telefoncu A: Immobilization of lipases on different carriers and their use in synthesis of pentyl isovalerates. Prep Biochem Biotechnol. 2004 May;34(2):169-78.


Porcine pancreatic lipase (PPL) and Candida cylindracea lipase (CCL) were immobilized on Celite and Amberlite IRA 938 by deposition from the aqueous solution by the addition of hexane.
2(0,0,0,2) Details
9928108 Kawakami K, Furukawa S: Catalytic properties of lipase entrapped in alkyl-substituted organic silicates. Ann N Y Acad Sci. 1998 Dec 13;864:323-6.

1(0,0,0,1) Details
10691609 Pinsirodom P, Parkin KL: Selectivity of celite-immobilized patatin (lipid acyl hydrolase) from potato (Solanum tuberosum L.) tubers in esterification reactions As influenced by water activity and glycerol analogues as alcohol acceptors. J Agric Food Chem. 2000 Feb;48(2):155-60.


The major protein band of 40-43 kDa on SDS-PAGE appeared to be patatin, and it stained positive for lipase activity on native PAGE.
1(0,0,0,1) Details
7763454 Mustranta A, Forssell P, Poutanen K: Applications of immobilized lipases to transesterification and esterification reactions in nonaqueous systems. Enzyme Microb Technol. 1993 Feb;15(2):133-9.

Lipases from Candida cylindracea, Aspergillus niger, and Pseudomonas fluorescens were immobilized by adsorption on anion-exchange resin and diatomaceous earth using buffer or hexane as a reaction medium.
Hexane was also a better immobilization medium for A. niger lipase.
1(0,0,0,1) Details
10440666 Ferrer M, Cruces MA, Bernabe M, Ballesteros A, Plou FJ: Lipase-catalyzed regioselective acylation of sucrose in two-solvent mixtures. Biotechnol Bioeng. 1999 Oct 5;65(1):10-6.

Several lipases were able to catalyze the transesterification, but that from Humicola lanuginosa (adsorbed on diatomaceous earth) was particularly useful.
1(0,0,0,1) Details
19789138 Verma ML, Azmi W, Kanwar SS: Synthesis of ethyl acetate employing celite-immobilized lipase of Bacillus cereus MTCC 8372. Acta Microbiol Immunol Hung. 2009 Sep;56(3):229-42.

In the present study, immobilization of a purified alkaline extra-cellular lipase of Bacillus cereus MTCC 8372 by adsorption on diatomaceous earth (celite) for synthesis of ethyl acetate via transesterification route was investigated.
36(0,1,1,6) Details
18576561 Wisdom RA, Dunnill P, Lilly MD: Enzymic interesterification of fats: Laboratory and pilot-scale studies with immobilized lipase from Rhizopus arrhizus. Biotechnol Bioeng. 1987 Jun;29(9):1081-5.

An immobilized lipase suitable for fat interesterification has been prepared by precipitation with acetone of a commercial lipase from Rhizopus arrhizus onto diatomaceous earth.
10(0,0,1,5) Details
18615605 Ison AP, Macrae AR, Smith CG, Bosley J: Mass transfer effects in solvent-free fat interesterification reactions: influences on catalyst design. Biotechnol Bioeng. 1994 Jan 20;43(2):122-30.

Initial studies on interesterification were carried out in solvent systems because the lipase was immobilized solely by adsorption onto particles of diatomaceous earth.
8(0,0,1,3) Details
11875972 Gorokhova IV, Ivanov AE, Zubov VP: [Coprecipitation of the Pseudomonas fluorescens lipase with hydrophobic compounds as an approach to its immobilization for catalysis in nonaqueous media]. Bioorg Khim. 2002 Jan-Feb;28(1):44-9.

5(0,0,0,5) Details
15974188 Sagiroglu A, Kilinc A, Telefoncu A: Preparation and properties of lipases immobilized on different supports. Artif Cells Blood Substit Immobil Biotechnol. 2004;32(4):625-36.


Porcine pancreatic lipase and Candida cylindracea lipase were immobilized on Celite and Amberlite IRA-938.
3(0,0,0,3) Details