Protein Information

Name estrogen receptor alpha
Synonyms ER; ERA; ER alpha; ERalpha; ESR; ESR 1; ESR1; ESRA…

Compound Information

Name silica gel
CAS silica gel

Reference List

PubMed Abstract RScore(About this table)
17685543 Cheng WY, Kuo YH, Huang CJ: Isolation and identification of novel estrogenic compounds in yam tuber (Dioscorea alata Cv. J Agric Food Chem. 2007 Sep 5;55(18):7350-8. Epub 2007 Aug 8.

In this study, the estrogenic activity of yam was examined and active compounds were isolated and identified based on ligand-dependent transcriptional activation through estrogen receptors.
Tainung No. 2 tuber was fractionated by repeated silica gel column chromatography.
3(0,0,0,3) Details
15833377 Kamiya M, Toriba A, Onoda Y, Kizu R, Hayakawa K: Evaluation of estrogenic activities of hydroxylated polycyclic aromatic hydrocarbons in cigarette smoke condensate. Food Chem Toxicol. 2005 Jul;43(7):1017-27.

Estrogenic activities of cigarette smoke condensates obtained from the extraction of particulate matters from mainstream and sidestream cigarette smoke with benzene/ethanol were evaluated by using a yeast two-hybrid assay system expressing human estrogen receptor alpha (hERalpha).
Then the neutral fractions were fractionated by medium-pressure liquid chromatography with silica gel column.
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18277611 Oh SM, Ryu BT, Chung KH: Identification of estrogenic and antiestrogenic activities of respirable diesel exhaust particles by bioassay-directed fractionation. Arch Pharm Res. 2008 Jan;31(1):75-82.

Bioassay-directed fractionation consists of a cell bioassay (E-SCREEN) in conjunction with acid-base partitioning (F1 and F2) and silica gel column fractionation of neutral fractions (F3-F7).
Estrogenic activity of CE and some fractions (F1, F2, F3, F5 and F6) was induced through estrogen receptor (ER)-mediated pathways.
1(0,0,0,1) Details
15886524 Jeon GC, Park MS, Yoon DY, Shin CH, Sin HS, Um SJ: Antitumor activity of spinasterol isolated from Pueraria roots. Exp Mol Med. 2005 Apr 30;37(2):111-20.

Finally, a transfection assay showed that this component had an estrogenic effect similar to 17beta - estradiol, which activates both estrogen receptor alpha (ERalpha) and ERbeta.
Dried, powdered plant material was extracted with 100% ethanol and further separated by concentration, filtration, and thin layer silica gel chromatography.
1(0,0,0,1) Details
17583877 Noguchi K, Toriba A, Chung SW, Kizu R, Hayakawa K: Identification of estrogenic/anti-estrogenic compounds in diesel exhaust particulate extract. Biomed Chromatogr. 2007 Nov;21(11):1135-42.

After separating DEPE into 11 fractions by liquid-liquid extraction, the neutral fraction (N) showed anti-estrogenic activity and the weak acid (phenol) fraction (WA (P)) showed estrogenic and anti-estrogenic activities by a yeast two-hybrid assay system expressing human estrogen receptor alpha.
Both fractions were thoroughly fractionated by silica gel column chromatography and reversed-phase HPLC.
1(0,0,0,1) Details