Protein Information

Name lipase
Synonyms HGL; HPL; HGL; Human giant larvae homolog; LGL 2; LGL2; LLGL 2; LLGL2…

Compound Information

Name silica gel
CAS silica gel

Reference List

PubMed Abstract RScore(About this table)
18051278 Lee DH, Kim JM, Shin HY, Kim SW: Optimization of lipase pretreatment prior to lipase immobilization to prevent loss of activity. J Microbiol Biotechnol. 2007 Apr;17(4):650-4.

In this study, Rhizopus oryzae lipase was pretreated before immobilization and then immobilized on a silica gel surface.
13(0,0,1,8) Details
17028778 Lee DH, Kim JM, Kang SW, Lee JW, Kim SW: Pretreatment of lipase with soybean oil before immobilization to prevent loss of activity. Biotechnol Lett. 2006 Dec;28(23):1965-9. Epub 2006 Sep 22.

The activity of the pretreated lipase immobilized covalently on a silica gel was 530 U/g-matrix, which is 16 times higher than that of the immobilized non-pretreated lipase.
10(0,0,1,5) Details
19390883 da Silva VC, Contesini FJ, de Oliveira Carvalho P: Enantioselective behavior of lipases from Aspergillus niger immobilized in different supports. J Ind Microbiol Biotechnol. 2009 Jul;36(7):949-54. Epub 2009 Apr 24.

Here, we report the performance in the resolution of racemic ibuprofen of a native enantioselective lipase from Aspergillus niger, free and immobilized in five types of support (Accurel EP-100, Amberlite MB-1, Celite, Montmorillonite K10 and Silica gel).
8(0,0,1,3) Details
16209548 Al-Zuhair S: Production of biodiesel by lipase-catalyzed transesterification of vegetable oils: a kinetics study. Biotechnol Prog. 2005 Sep-Oct;21(5):1442-8.

The kinetic parameters were estimated by fitting the experimental data of the enzymatic reaction of sunflower oil by two types of lipases, namely, Rhizomucor miehei lipase (RM) immobilized on ion-exchange resins and Thermomyces lanuginosa lipase (TL) immobilized on silica gel.
8(0,0,1,3) Details
19360797 Ganesan A, Moore BD, Kelly SM, Price NC, Rolinski OJ, Birch DJ, Dunkin IR, Halling PJ: Optical spectroscopic methods for probing the conformational stability of immobilised enzymes. Chemphyschem. 2009 Jul 13;10(9-10):1492-9.

Their applicability is demonstrated using subtilisin Carlsberg (SC) immobilised on silica gel and Candida antartica lipase B immobilised on Lewatit VP.OC 1600 (Novozyme 435).
6(0,0,1,1) Details
20352971 Zheng S, Ren C, Han S, Lin Y: [Synthesis of glucose laurate monoester catalyzed by Candida antarctica lipase B-displaying Pichia pastoris whole-cells]. Sheng Wu Gong Cheng Xue Bao. 2009 Dec;25(12):1933-9.

The product glucose laurate monoester was purified by silica gel column chromatography and preparative liquid chromatography, and identified by liquid chromatography-mass spectrometry.
2(0,0,0,2) Details
17096388 Petersson AE, Adlercreutz P, Mattiasson B: A water activity control system for enzymatic reactions in organic media. Biotechnol Bioeng. 2007 Jun 1;97(2):235-41.

The control system consists of a water activity sensor in the headspace of a jacketed glass reactor (equipped with narrow steel tubes to introduce air), gas-washing bottles containing blue silica gel (a (w)=0) and water (a (w)=1), a PC to monitor water activity and a programmable logic controller (PLC) to control the water activity.
Synthesis of cetyl palmitate, under controlled water activity and catalysed by two different lipase preparations, namely, Novozym 435 (immobilised Candida antarctica lipase B) and immobilised Candida rugosa lipase, were also performed.
2(0,0,0,2) Details
18327546 Alloue WA, Destain J, El Medjoub T, Ghalfi H, Kabran P, Thonart P: Comparison of Yarrowia lipolytica lipase immobilization yield of entrapment, adsorption, and covalent bond techniques. Appl Biochem Biotechnol. 2008 Jul;150(1):51-63. Epub 2008 Mar 8.

The immobilization by adsorption was carried out on both celite and silica gel.
5(0,0,0,5) Details
15899531 Karboune S, Safari M, Lue BM, Yeboah FK, Kermasha S: Lipase-catalyzed biosynthesis of cinnamoylated lipids in a selected organic solvent medium. J Biotechnol. 2005 Sep 29;119(3):281-90.

With cinnamic acid to triolein ratios of 1:4.5 and 1:6.0, an increase in the bioconversion yield of cinnamoylated lipids to 55% was achieved by adding 2.2 mgmL (-1) silica gel to the reaction mixture.
2(0,0,0,2) Details
19222220 Kato E, Tokunaga Y, Sakan F: Stilbenoids isolated from the seeds of Melinjo (Gnetum gnemon L.) and their biological activity. J Agric Food Chem. 2009 Mar 25;57(6):2544-9.

A 50% EtOH extract of the dried endosperms of melinjo (Gnetum gnemon L.) was purified by a combination of column chromatography, including highly porous polymer, octadecylated silica (ODS), hydroxypropylated dextran (LH-20), and silica gel, resulting in the isolation of a new stilbenoid 1, named gnetin L, along with five previously identified stilbenoids 2-6 (gnetin C, gnemonosides A, C, and D, and resveratrol).
Inhibition of lipase from porcine pancreas was recognized in four stilbenoids excluding gnemonoside A (3) and resveratrol (6).
2(0,0,0,2) Details
15830930 Piyatheerawong W, Iwasaki Y, Yamane T: Direct separation of regio- and enantiomeric isomers of diacylglycerols by a tandem column high-performance liquid chromatography. J Chromatogr A. 2005 Mar 18;1068(2):243-8.

The method employs a tandem column system, in which two different columns (a conventional silica gel column and a chiral stationary phase column) are connected in series.
Two isomeric mixtures of DAGs (i.e., dicapryloylglycerol and dioleoylglycerol) and lipase-catalyzed reaction mixtures were successfully resolved on the tandem column HPLC system without any derivatization prior to the analysis.
2(0,0,0,2) Details
19727949 Ko HS, Jin RD, Krishnan HB, Lee SB, Kim KY: Biocontrol ability of Lysobacter antibioticus HS124 against Phytophthora blight is mediated by the production of 4-hydroxyphenylacetic acid and several lytic enzymes. Curr Microbiol. 2009 Dec;59(6):608-15. Epub 2009 Aug 29.

L. antibioticus HS124 produced lytic enzymes such as chitinase, beta-1,3-glucanase, lipase, protease, and an antibiotic compound.
This antibiotic compound was purified by diaion HP-20, silica gel, sephadex LH-20 column chromatography and high performance liquid chromatography.
1(0,0,0,1) Details
18703340 Yoshimura Y, Ohara C, Imahori T, Saito Y, Kato A, Miyauchi S, Adachi I, Takahata H: Synthesis of both enantiomers of hydroxypipecolic acid derivatives equivalent to 5-azapyranuronic acids and evaluation of their inhibitory activities against glycosidases. Bioorg Med Chem. 2008 Sep 1;16(17):8273-86. Epub 2008 Jun 13.

Cross aldol reaction of N-Boc-allylglycine derivative with acrolein followed by the ring-closing metathesis gave 4 and 5 as a mixture of diastereomers which could be separated by silica gel column chromatography.
By employing lipase-catalyzed kinetic resolution, the synthesis of both L- and D-isomers of 3,4,5-trihydroxy- and 3-hydroxypipecolic acids was achieved.
1(0,0,0,1) Details
17692859 Deng L, Nakano H, Iwasaki Y: Direct separation of regioisomers and enantiomers of monoacylglycerols by tandem column high-performance liquid chromatography. J Chromatogr A. 2007 Sep 21;1165(1-2):93-9. Epub 2007 Aug 2.

The method employs a tandem column system, in which two different columns (a conventional silica gel column and an enantioselective column) are connected in series.
By means of the established analytical method, the reaction course of Candida antarctica lipase B (CALB)-mediated esterification of glycerol with oleic acid was monitored.
1(0,0,0,1) Details
19001769 Yu L, Shirai N, Suzuki H, Hosono T, Nakajima Y, Kajiwara M, Takatori K: Effect of lipid extracted from tsao-ko (Amomum tsao-ko Crevost et Lemaire) on digestive enzyme activity, antioxidant activity, plasma and liver lipids, and blood glucose levels of mice. J Nutr Sci Vitaminol. 2008 Oct;54(5):378-83.

Lipids extracted from tsao-ko were separated into three fractions with silica gel column chromatography and fed to mice (3 mo old) for 90 d to clarify their inhibitory activity on digestive enzyme activity.
Although CF and AF slightly inhibited the activities of alpha-glucosidase, alpha-amylase, and lipase, intakes of these fractions had little influence on plasma and liver lipid concentrations when compared with the control diet.
1(0,0,0,1) Details
17882456 Kumari A, Mahapatra P, Kumar GV, Banerjee R: Comparative study of thermostability and ester synthesis ability of free and immobilized lipases on cross linked silica gel. Bioprocess Biosyst Eng. 2008 Jun;31(4):291-8. Epub 2007 Sep 20.

A novel support has been utilized for immobilization of lipase, which was prepared by amination of silica with ethanolamine followed by cross linking with glutaraldehyde.
1(0,0,0,1) Details
17705140 Ekanem AP, Wang M, Simon JE, Moreno DA: Antiobesity properties of two African plants (Afromomum meleguetta and Spilanthes acmella) by pancreatic lipase inhibition. Phytother Res. 2007 Dec;21(12):1253-5.

The two plants were extracted with 70% ethanol by sonication, fractionated on silica gel and tested at concentrations in the range 0.75-2.0 mg/mL.
Lipase inhibitory activities of 90% and 40% were observed in A. meleguetta and S. acmella, respectively.
1(0,0,0,1) Details