Protein Information

Name immunoglobulins (protein family or complex)
Synonyms Immunoglobulin; Immunoglobulins

Compound Information

Name sodium thiocyanate
CAS sodium thiocyanate

Reference List

PubMed Abstract RScore(About this table)
2539075 Myers TJ, Schat KA, Mockett AP: Development of immunoglobulin class-specific enzyme-linked immunosorbent assays for measuring antibodies against avian rotavirus. Avian Dis. 1989 Jan-Mar;33(1):53-9.

Treatment of purified rotavirus with sodium thiocyanate before coating the plate improved the rotavirus-specific reading in the indirect assay.
2(0,0,0,2) Details
8500885 Ak M, Bower JH, Hoffman SL, Sedegah M, Lees A, Carter M, Beaudoin RL, Charoenvit Y: Monoclonal antibodies of three different immunoglobulin G isotypes produced by immunization with a synthetic peptide or native protein protect mice against challenge with Plasmodium yoelii sporozoites. Infect Immun. 1993 Jun;61(6):2493-7.

There was no correlation between antibody level at challenge as determined by immunofluorescent antibody test against sporozoites or enzyme-linked immunosorbent assay against (QGPGAP) 2 or apparent antibody avidity for (QGPGAP) 2 by sodium thiocyanate elution assay.
2(0,0,0,2) Details
6452414 Moore DG, Earhart CF: Specific inhibition of Escherichia coli ferrienterochelin uptake by a normal human serum immunoglobulin. Infect Immun. 1981 Feb;31(2):631-5.

Elution with 3 M sodium thiocyanate or 13 mM 2,3-dihydroxybenzoic acid proved successful.
3(0,0,0,3) Details
11247642 Bouvet JP, Stahl D, Rose S, Quan CP, Kazatchkine MD, Kaveri SV: Induction of natural autoantibody activity following treatment of human immunoglobulin with dissociating agents. J Autoimmun. 2001 Mar;16(2):163-72.

Treatment of normal polyclonal human IgG and of F (ab') 2 fragments of IgG with 6.0 M urea, 1.3 M sodium thiocyanate or with acidic buffers (pH 2.0), resulted in a dramatic and selective enhancement of the preexisting antibody reactivity with self antigens.
1(0,0,0,1) Details
17303253 Chapman MD, Thompson EJ, Candler PM, Dale RC, Church AJ, Giovannoni G: Quantitative demonstration of intrathecal synthesis of high affinity immunoglobulin G in herpes simplex encephalitis using affinity-mediated immunoblotting. J Neuroimmunol. 2007 Apr;185(1-2):130-5. Epub 2007 Feb 14.

The samples were investigated using affinity-mediated immunoblotting followed by incubation with sodium thiocyanate.
1(0,0,0,1) Details
6421279 Pegg AE, Seely JE, Persson L, Herlyn M, Ponsell K, O'Brien TG: Studies of mammalian ornithine decarboxylase using a monoclonal antibody. Biochem J. 1984 Jan 1;217(1):123-8.

A monoclonal antibody of the immunoglobulin M class was produced against mouse kidney ornithine decarboxylase.
The active enzyme could then be eluted in a highly purified form by 1.0M-sodium thiocyanate.
1(0,0,0,1) Details
108802 Chalon MP, Milne RW, Vaerman JP: Interactions between mouse immunoglobulins and staphylococcal protein A. . Scand J Immunol. 1979;9(4):359-64.

When a gradient from 0 to 3 M sodium thiocyanate was started after the major peak of unbound proteins, all IgG1 was eluted before IgG2 and IgG3.
1(0,0,0,1) Details
16148167 Romero-Steiner S, Holder PF, Gomez de Leon P, Spear W, Hennessy TW, Carlone GM: Avidity determinations for Haemophilus influenzae Type b anti-polyribosylribitol phosphate antibodies. Clin Diagn Lab Immunol. 2005 Sep;12(9):1029-35.

We evaluated three approaches for the determination of antibody avidity for immunoglobulin G (IgG).
We determined that elution of low-avidity antibodies present in multiple dilutions of the serum sample by a single concentration of a chaotrope (0.15 M sodium thiocyanate [NaSCN]) was optimal for the determination of avidity measurements throughout a wide range of IgG concentrations (0.94 to 304.6 microg/ml).
1(0,0,0,1) Details
18771565 Kavanagh-Wright L, Smith TP, Gibney J, McKenna TJ: Characterization of macroprolactin and assessment of markers of autoimmunity in macroprolactinaemic patients. Clin Endocrinol. 2009 Apr;70(4):599-605. Epub 2008 Sep 2.

OBJECTIVE: It has been reported that macroprolactin is a complex of PRL and an immunoglobulin G (IgG).
DESIGN: PRL was measured in sera prior to and following gel filtration chromatography, ultrafiltration, treatment with protein A-sepharose, protein G-sepharose, antihuman IgG-agarose and sodium thiocyanate (NaSCN).
1(0,0,0,1) Details
116576 Warr GW, Hart IR: Binding of canine IgM and IgG to protein A of Staphylococcus aureus: a simple method for the isolation of canine immunoglobulins from serum and the lymphocyte surface. Am J Vet Res. 1979 Jul;40(7):922-6.

Attempts to elute differentially IgG and IgM from protein A-Sepharose columns, using gradients of pH or the chaotropic agent sodium thiocyanate, were unsuccessful.
1(0,0,0,1) Details
659858 Mackenzie MR, Warner NL, Mitchell GF: The binding of murine immunoglobulins to staphylococcal protein A. . J Immunol. 1978 May;120(5):1493-6.

Differential elution by sodium thiocyanate gradients is described.
1(0,0,0,1) Details
3748998 Jenny R, Church W, Odegaard B, Litwiller R, Mann K: Purification of six human vitamin K-dependent proteins in a single chromatographic step using immunoaffinity columns. Prep Biochem. 1986;16(3):227-45.

Purified monoclonal antibodies and a purified burro polyclonal anti-prothrombin immunoglobulin were individually coupled to Sepharose and used in a tandem series of columns to purify each of the vitamin K-dependent proteins from eluates of barium citrate precipitates of plasma.
The proteins were eluted from the columns by sodium thiocyanate and retained functional activity following dialysis.
1(0,0,0,1) Details
11687449 Romero-Steiner S, Fernandez J, Biltoft C, Wohl ME, Sanchez J, Feris J, Balter S, Levine OS, Carlone GM: Functional antibody activity elicited by fractional doses of Haemophilus influenzae type b conjugate vaccine (polyribosylribitol phosphate-tetanus toxoid conjugate). Clin Diagn Lab Immunol. 2001 Nov;8(6):1115-9.

We evaluated the functional activities of antibodies, serum bactericidal activity (SBA), and immunoglobulin G (IgG) antibody avidity indices, using sodium thiocyanate (NaSCN) elution, elicited after vaccination with fractional doses of the Haemophilus influenzae type b conjugate (polyribosylribitol phosphate [PRP] conjugated to tetanus toxoid [PRP-T]) vaccine.
31(0,1,1,1) Details
7055589 Rousseaux J, Aubert JP, Loucheux-Lefebvre MH: Comparative study of the conformational features of rat immunoglobulin G subclasses by circular dichroism. Biochim Biophys Acta. 1982 Feb 4;701(1):93-101.

4(0,0,0,4) Details