Protein Information

Name estrogen receptor
Synonyms ER; ERA; ER alpha; ERalpha; ESR; ESR 1; ESR1; ESRA…

Compound Information

Name sodium thiocyanate
CAS sodium thiocyanate

Reference List

PubMed Abstract RScore(About this table)
4021495 de Boer W, Ab G, Gruber M: Estrogen receptor in chicken oviduct: receptor dissociation kinetics and transformation. J Steroid Biochem. 1985 Jul;23(1):9-18.

4(0,0,0,4) Details
6838903 Okulicz WC, Boomsma RA, MacDonald RG, Leavitt WW: Conditions for the measurement of nuclear estrogen receptor at low temperature. Biochim Biophys Acta. 1983 May 4;757(1):128-36.

3(0,0,0,3) Details
7001472 Greene GL, Nolan C, Engler JP, Jensen EV: Monoclonal antibodies to human estrogen receptor. . Proc Natl Acad Sci U S A. 1980 Sep;77(9):5115-9.

Elution with 50 micro M [3H] estradiol in 10% (vol/vol) dimethyl formamide/0.5 M sodium thiocyanate gave 40% recovery of [3H] estradiol-estrophilin showing 14% of the specific radioactivity expected for the pure complex.
3(0,0,0,3) Details
6207854 Moncharmont B, Anderson WL, Rosenberg B, Parikh I: Interaction of estrogen receptor of calf uterus with a monoclonal antibody: probing of various molecular forms. Biochemistry. 1984 Aug 14;23(17):3907-12.

2(0,0,0,2) Details
1491008 Inano K, Ishida T, Omata S, Horigome T: In vitro formation of estrogen receptor-heat shock protein 90 complexes. . J Biochem. 1992 Oct;112(4):535-40.

2(0,0,0,2) Details
4040190 Leavitt WW, Okulicz WC: Progesterone control of nuclear estrogen receptor: demonstration in hamster uterus during the estrous cycle and pseudopregnancy using a new exchange assay. J Steroid Biochem. 1985 May;22(5):583-8.

2(0,0,0,2) Details
7287657 Fukai F, Murayama A: Cytoplasmic estrogen receptor system of gilt uterus. J Biochem. 1981 Jun;89(6):1829-37.

This molecule was further designated as vero-ER. "8S" ER-forming factor [("8S"ER)-FF] (Stokes radius, 51 A) was separated from the cytosol under hypotonic (low salt) conditions, and this was further dissociated into component A ["5S" ER-forming factor, ("5S"ER)-FF] (Stokes radius, 37 A) and component B (Stokes radius, 18.5 A) in the presence of sodium thiocyanate.
2(0,0,0,2) Details
7265965 Sato B, Nishizawa Y, Maeda Y, Noma K, Matsumoto K, Yamamura Y: Detection of estrogen receptor-like high affinity components by exposure of cytosols from mouse Leydig cell tumor or male rat liver to chaotropic salts. J Steroid Biochem. 1981 Jun;14(7):619-24.

1(0,0,0,1) Details
3825817 Nakahara M: [Purification of the human prostate androgen receptor. Hinyokika Kiyo. 1986 Nov;32(11):1689-99.


The effect of NaSCN on the AR was therefore different from that on the estrogen receptors.
1(0,0,0,1) Details
6243476 Sica V, Puca GA, Molinari AM, Buonaguro FM, Bresciani F: Effect of chemical perturbation with NaSCN on receptor-estradiol interaction. Biochemistry. 1980 Jan 8;19(1):83-8.

When 0.5 M sodium thiocyanate is added to uterine cytosol previously labeled with excess [3H]-17 beta-estradiol, no change can be detected in the steady-state cytosol concentration of [3H] estradiol-receptor complex for at least 20 h at 4 degrees C.
This effect of NaSCN can be exploited to assay preformed estrogen-receptor complex by exchange with [3H] estradiol at low temperature.
1(0,0,0,1) Details
1583622 Poirot M, Chailleux C, Bayard F, Faye JC: Cytosolic type II estrogen binding site in rat uterus: specific photolabeling with estrone. J Recept Res. 1992;12(2):217-31.

This treatment depleted type I estrogen receptors from the cytosol (by 90%) and raised levels of type II sites in the nucleus without affecting cytosolic type II levels.
The type II estradiol binding sites were distinguished from the type I sites on the basis of their dissociation kinetics, pH-sensitivity and their behavior towards potassium chloride, somatostatin, sodium thiocyanate, sulfhydryl reagents and ammonium sulfate precipitation.
1(0,0,0,1) Details
178358 Sica V, Nola E, Puca GA, Bresciani F: Estrogen binding proteins of calf uterus. Biochemistry. 1976 May 4;15(9):1915-23.

Sodium thiocyanate up to 0.5 M is compatible with a stable estradiol-t-receptor complex during sucrose gradient centrifugation; however, the maximum permissible concentration in 0.1 M during Sephadex G-100 and G-200 chromatography.
When NaSCN 0.1 M is added to low-salt cytosol (approximately 7 mg of protein/ml); (1) age-dependent aggregation of receptor is inhibited; (2) peaks of estrogen-binding activity in sucrose gradients and on Sephadex chromatography are sharp; (3) instead of the usual larger molecular states ("8S") found in low salt, most of estrogen receptor is under the following form: 4.1S; Stokes radius, 36 A; mol wt 61 000; flfo, 1.25; homogeneous at electrofocusing, with isoelectric point at 6.0.
1(0,0,0,1) Details
7157436 Belisle S, Lehoux JG, Bellabarba D: Exchange assays using sodium thiocyanate to measure total nuclear content of estrogen receptors in the hypothalamic pituitary axis of mice. Steroids. 1982 Jun;39(6):643-55.
13(0,0,2,3) Details
3400183 Ignatenko LL, Mataradze GD, Buniatian AF: [Determination of free and bound forms of estrogen receptors in rat liver cytosol using a method of ligand turnover at low temperature]. Vopr Med Khim. 1988 Mar-Apr;34(2):125-8.

A procedure is described for estimation of free and bound estrogen receptors in rat liver cytosol at low temperature using ligand turnover in presence of sodium thiocyanate.
84(1,1,1,4) Details
2337659 Ignatenko LL, Mataradze GD, Buniatian AF, Rozen VB: [Sex differences in the level of cytosol estrogen receptors in the rat liver]. Biull Eksp Biol Med. 1990 Feb;109(2):183-6.

The procedure designed for the estimation of estrogen receptors (ER) in rat liver cytosol using sodium thiocyanate was shown to be useful for differential quantification of the ER level in liver cytosol of male rats, containing the unusual estrogen-binding protein.
32(0,1,1,2) Details
7213602 Sica V, Weisz A, Petrillo A, Armetta I, Puca GA: Assay of total estradiol receptor in tissue homogenate and tissue fractions by exchange with sodium thiocyanate at low temperature. Biochemistry. 1981 Feb 17;20(4):686-93.
7(0,0,1,2) Details
6509444 Concolino G, Giuffre R, Margiotta G, Marocchi A, Conti C: Steroid receptors in the pathogenesis of chronic subdural hematoma. . Clin Neuropharmacol. 1984;7(4):343-6.

In an attempt to increase our understanding of the physiopathogenesis of CSDH, total estrogen receptor (ER) was measured in HEM specimens from four male patients by a sodium thiocyanate exchange assay and cytosol progesterone receptor (PRc) in three specimens by a dextran-coated charcoal adsorption assay.
6(0,0,1,1) Details