Protein Information

Name GAL4
Synonyms Antigen NY CO 27; GAL 4; GAL4; Galectin 4; L 36 lactose binding protein; L36LBP; LGALS 4; LGALS4…

Compound Information

Name methoxyfenozide
CAS 3-methoxy-2-methylbenzoic acid 2-(3,5-dimethylbenzoyl)-2-(1,1-dimethylethyl)hydrazide

Reference List

PubMed Abstract RScore(About this table)
16412090 Tavva VS, Dinkins RD, Palli SR, Collins GB: Development of a methoxyfenozide-responsive gene switch for applications in plants. Plant J. 2006 Feb;45(3):457-69.

The receptor constructs were prepared by fusing DEF domains (hinge region plus ligand-binding domain) of CfEcR to the GAL4 DNA-binding domain and EF domains (ligand-binding domain) of ultraspiracle from Choristoneura fumiferana (CfUSP) or RXR from Locusta migratoria (LmRXR), Mus musculus (MmRXR) or Homo sapiens (HsRXR) to the VP16 activation domain.
2(0,0,0,2) Details
12437077 Unger E, Cigan AM, Trimnell M, Xu RJ, Kendall T, Roth B, Albertsen M: A chimeric ecdysone receptor facilitates methoxyfenozide-dependent restoration of male fertility in ms45 maize. Transgenic Res. 2002 Oct;11(5):455-65.

A high frequency of phenotypic complementation was observed when either C1-GAL4 or VP16-GAL4 activators were transcribed by promoters that expressed at a stage of anther development that precedes the early vacuolate stage of microsporogenesis.
2(0,0,0,2) Details
12650528 Padidam M, Gore M, Lu DL, Smirnova O: Chemical-inducible, ecdysone receptor-based gene expression system for plants. Transgenic Res. 2003 Feb;12(1):101-9.

We have developed an inducible gene expression system with potential for field application using the ecdysone receptor (EcR) from the spruce budworm and the non-steroidal EcR agonist, methoxyfenozide.
Chimeric transcription activators were constructed with EcR ligand binding domain, GAL4 and LexA DNA binding domains, and VP16 activation domain.
1(0,0,0,1) Details
17570490 Tavva VS, Palli SR, Dinkins RD, Collins GB: Applications of EcR gene switch technology in functional genomics. Arch Insect Biochem Physiol. 2007 Jul;65(3):164-79.

The ecdysone receptor gene switch is one of the best inducible gene regulation systems available, because the chemical, methoxyfenozide, required for its regulation is registered for field use.
In a two-hybrid switch format, the GAL4 DNA binding domain (GAL4 DBD) was fused to the ligand binding domain (LBD) of the Choristoneura fumiferana ecdysone receptor (CfEcR); and, the VP16 activation domain (VP16 AD) was fused to the LBD of Locust migratoria retinoid X receptor (LmRXR).
1(0,0,0,1) Details