| 19584536 |
Fu YY, Li ZG, Yang YW, Deng W, Duan W, Miao Q, Wang ZK, Xia YX: Isolation of single chain variable fragments against six esters of pyrethrins by subtractive phage display. Biosci Biotechnol Biochem. 2009 Jul;73(7):1541-9. Epub 2009 Jul 7.
Here we describe the generation of monomeric and dimeric single chain variable fragments (scFvs) with affinity for six esters of pyrethrins using a subtractive phage display technology.
After four rounds of panning, phage ELISA demonstrated that three clones (E2, F2, and H7) showed higher affinity from the long-linker library, and clones (h6, a5) exhibited better antibody activity to pyrethrin I and II from the short-linker library. |
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