Protein Information

Name alcohol dehydrogenase (protein family or complex)
Synonyms ADH; alcohol dehydrogenase; alcohol dehydrogenases

Compound Information

Name rotenone
CAS

Reference List

PubMed Abstract RScore(About this table)
562603 Thurman RG, Brentzel HJ, McKenna WR: Common mechanism for the adaptive increase in hepatic ethanol and acetaldehyde metabolism due to chronic pretreatment with ethanol. Adv Exp Med Biol. 1977;85A:237-45.

Ethanol uptake in both ethanol-treated and control livers was similarly sensitive to inhibition by 4-methylpyrazole, rotenone, and antimycin A.
As a consequence of these events, a greater rate of NADH reoxidation occurs, resulting in a greater rate of production of NAD+ which stimulates ethanol oxidation via alcohol dehydrogenase and acetaldehyde oxidation via aldehyde dehydrogenase (s).
1(0,0,0,1) Details
1449272 Kumar S, Tripathi LM, Sagar P: Oxido-reductive functions of Entamoeba histolytica in relation to virulence. Ann Trop Med Parasitol. 1992 Jun;86(3):239-48.

Both NBT-reducing capability and alcohol dehydrogenase activities were higher in the more virulent IP:106 strain, and they increased with time in cultures of NIH:200 in a cholesterol-enriched environment.
Conversion of the dye to formazan was strongly inhibited by -SH blocking agents, but was not influenced by rotenone and antimycin A.
1(0,0,0,1) Details
16934215 Gonzalez B, Martinez S, Chavez JL, Lee S, Castro NA, Dominguez MA, Gomez S, Contreras ML, Kennedy C, Escamilla JE: Respiratory system of Gluconacetobacter diazotrophicus PAL5. Biochim Biophys Acta. 2006 Dec;1757(12):1614-22. Epub 2006 Jul 8.


Strong inhibition by rotenone and capsaicin and resistance to flavone indicated that NADH-quinone oxidoreductase is a NDH-1 type enzyme.
0(0,0,0,0) Details
16344601 French SW: The pathogenesis and significance of the urinary alcohol cycle in rats fed ethanol intragastrically. Alcohol Clin Exp Res. 2005 Nov;29(11 Suppl):158S-161S.

The third element essential to the cycle is the generation of NAD to support the oxidation of alcohol by alcohol dehydrogenase.
When complex I (NADH dehydrogenase) of the mitochondrial electron transport chain is inhibited by feeding rotenone, the cycle is totally eliminated and blood alcohol levels remain constant at 200 mg/%.
1(0,0,0,1) Details
4292808 Balcavage WX, Mattoon JR: Effect of rotenone on the alcohol dehydrogenase of yeast mitochondria. Nature. 1967 Jul 8;215(5097):166-8.
6(0,0,1,1) Details
8135551 Kukielka E, Dicker E, Cederbaum AI: Increased production of reactive oxygen species by rat liver mitochondria after chronic ethanol treatment. Arch Biochem Biophys. 1994 Mar;309(2):377-86.

Oxidation of ethanol by alcohol dehydrogenase generates NADH, and NADH-dependent production of reactive oxygen species by various organelles is increased after chronic ethanol treatment.
Modifiers of mitochondrial metabolism such as rotenone, cyanide, or an uncoupling agent, had no effect on mitochondrial production of reactive oxygen intermediates.
1(0,0,0,1) Details