Protein Information

Name beta glucuronidase
Synonyms Beta glucuronidase; Beta G1; Beta glucuronidase precursor; GUSB; MPS 7; MPS7; Beta G1s; Beta glucuronidase precursors

Compound Information

Name IAA
CAS

Reference List

PubMed Abstract RScore(About this table)
14605219 Nakamura A, Higuchi K, Goda H, Fujiwara MT, Sawa S, Koshiba T, Shimada Y, Yoshida S: Brassinolide induces IAA5, IAA19, and DR5, a synthetic auxin response element in Arabidopsis, implying a cross talk point of brassinosteroid and auxin signaling. Plant Physiol. 2003 Dec;133(4):1843-53. Epub 2003 Nov 6.


We also found that a fusion of DR5, a synthetic auxin response element, with the GUS (beta-glucuronidase) gene was induced with similar kinetics to those of the IAA5 and IAA19 genes in response to both IAA and BL treatment of transgenic plants.
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10859183 Tuominen H, Puech L, Regan S, Fink S, Olsson O, Sundberg B: Cambial-region-specific expression of the Agrobacterium iaa genes in transgenic aspen visualized by a linked uidA reporter gene. Plant Physiol. 2000 Jun;123(2):531-42.

The rate-limiting bacterial IAA-biosynthetic gene iaaM and the reporter gene for beta-glucuronidase (GUS), uidA, were each fused to the cambial-region-specific Agrobacterium rhizogenes rolC promoter and linked on the same T-DNA.
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10608655 Yi HC, Joo S, Nam KH, Lee JS, Kang BG, Kim WT: Auxin and brassinosteroid differentially regulate the expression of three members of the 1-aminocyclopropane-1-carboxylate synthase gene family in mung bean (Vigna radiata L.). Plant Mol Biol. 1999 Nov;41(4):443-54.


The VR-ACS7 gene was isolated, and chimeric fusion between the 2.4 kb 5'-upstream region and the beta-glucuronidase (GUS) reporter gene was constructed and introduced into Nicotiana tabacum.
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19458116 Staswick PE: The tryptophan conjugates of jasmonic and indole-3-acetic acids are endogenous auxin inhibitors. Plant Physiol. 2009 Jul;150(3):1310-21. Epub 2009 May 20.


Trp conjugates inhibited IAA-stimulated lateral root production and DR5-beta-glucuronidase gene expression.
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17406771 Govindarajan M, Balandreau J, Kwon SW, Weon HY, Lakshminarasimhan C: Effects of the inoculation of Burkholderia vietnamensis and related endophytic diazotrophic bacteria on grain yield of rice. Microb Ecol. 2008 Jan;55(1):21-37. Epub 2007 Apr 4.


Transverse sections of roots, 15 days after inoculation, revealed beta-glucuronidase (GUS) activity within many of the cortical intercellular spaces next to the stele and within the aerenchyma.
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16507092 Tanaka M, Takei K, Kojima M, Sakakibara H, Mori H: Auxin controls local cytokinin biosynthesis in the nodal stem in apical dominance. Plant J. 2006 Mar;45(6):1028-36.


Furthermore, beta-glucuronidase expression, under the control of the PsIPT2 promoter region in transgenic Arabidopsis, was repressed by an IAA.
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12624772 Aloni R, Schwalm K, Langhans M, Ullrich CI: Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis. Planta. 2003 Mar;216(5):841-53. Epub 2002 Nov 26.


During early stages of primordium development, leaf apical dominance was evident from strong beta-glucuronidase activity in the elongating tip, possibly suppressing the production of free auxin in the leaf tissues below it.
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12226500 Avsian-Kretchmer O, Cheng JC, Chen L, Moctezuma E, Sung ZR: Indole acetic acid distribution coincides with vascular differentiation pattern during Arabidopsis leaf ontogeny. Plant Physiol. 2002 Sep;130(1):199-209.


Using immunocytochemistry and the IAA-driven beta-glucuronidase (GUS) activity of Aux/IAA promoter::GUS constructs to detect IAA distribution, we investigated the role of polar auxin transport in vascular differentiation during leaf development in Arabidopsis.
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15155875 Rampey RA, LeClere S, Kowalczyk M, Ljung K, Sandberg G, Bartel B: A family of auxin-conjugate hydrolases that contributes to free indole-3-acetic acid levels during Arabidopsis germination. Plant Physiol. 2004 Jun;135(2):978-88. Epub 2004 May 21.


We examined amidohydrolase gene expression using northern and promoter-beta-glucuronidase analyses and found overlapping but distinct patterns of expression.
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15466221 Tsuchisaka A, Theologis A: Unique and overlapping expression patterns among the Arabidopsis 1-amino-cyclopropane-1-carboxylate synthase gene family members. Plant Physiol. 2004 Oct;136(2):2982-3000. Epub 2004 Oct 1.


Transgenic Arabidopsis lines were constructed expressing the beta-glucuronidase (GUS) and green fluorescence protein (GFP) reporter genes from the promoter of each of the gene family members to determine their patterns of expression during plant development.
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9761792 Oono Y, Chen QG, Overvoorde PJ, Kohler C, Theologis A: age Mutants of Arabidopsis exhibit altered auxin-regulated gene expression. Plant Cell. 1998 Oct;10(10):1649-62.


An Arabidopsis transgenic line was constructed expressing beta-glucuronidase (GUS) via the auxin-responsive domains (AuxRDs) A and B (BA-GUS) of the PS-IAA4/5 gene in an indoleacetic acid (IAA)-dependent fashion.
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17434984 Zhang ZB, Yang G, Arana F, Chen Z, Li Y, Xia HJ: Arabidopsis inositol polyphosphate 6-/3-kinase (AtIpk2beta) is involved in axillary shoot branching via auxin signaling. Plant Physiol. 2007 Jun;144(2):942-51. Epub 2007 Apr 13.


The AtIpk2beta promoter/beta-glucuronidase (GUS) fusion (AtIpk2betaGUS) expression pattern is similar to that of the auxin reporter DR5GUS.
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11525507 Vorwerk S, Biernacki S, Hillebrand H, Janzik I, Muller A, Weiler EW, Piotrowski M: Enzymatic characterization of the recombinant Arabidopsis thaliana nitrilase subfamily encoded by the NIT2/NIT1/NIT3-gene cluster. Planta. 2001 Mar;212(4):508-16.


A potential role for NIT2 in breaking seed dormancy in A. thaliana by low temperatures (stratification), however, was ruled out, although NIT2 was the predominantly expressed nitrilase isoform in developing embryos and in germinating seeds, as judged from an analysis of beta-glucuronidase reporter gene expression under the control of the promoters of the four isogenes.
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15889272 Rock CD, Sun X: Crosstalk between ABA and auxin signaling pathways in roots of Arabidopsis thaliana (L.) Heynh. Planta. 2005 Sep;222(1):98-106. Epub 2005 May 12.


The Daucus carota (L.) Dc3 promoter: uidA (beta-glucuronidase: GUS) chimaeric reporter (ProDc3:GUS) is induced by ABA, osmoticum, and the auxin indole-3-acetic acid (IAA) in vegetative tissues of transgenic Arabidopsis thaliana (L.) Heynh.
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16367964 Nakamura A, Nakajima N, Goda H, Shimada Y, Hayashi K, Nozaki H, Asami T, Yoshida S, Fujioka S: Arabidopsis Aux/IAA genes are involved in brassinosteroid-mediated growth responses in a manner dependent on organ type. Plant J. 2006 Jan;45(2):193-205.


An inhibitor of BR biosynthesis, brassinazole, inhibited auxin induction of the DR5-GUS gene, which consists of a synthetic auxin-response element, a minimum promoter, and a beta-glucuronidase.
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14682616 Pacios-Bras C, Schlaman HR, Boot K, Admiraal P, Langerak JM, Stougaard J, Spaink HP: Auxin distribution in Lotus japonicus during root nodule development. Plant Mol Biol. 2003 Aug;52(6):1169-80.


For this work, Lotus japonicus transgenic plants were constructed expressing a fusion reporter gene consisting of the genes beta-glucuronidase (gus) and green fluorescent protein (gfp) under control of the soybean auxin-responsive promoter GH3.
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17220367 Yamamoto Y, Kamiya N, Morinaka Y, Matsuoka M, Sazuka T: Auxin biosynthesis by the YUCCA genes in rice. Plant Physiol. 2007 Mar;143(3):1362-71. Epub 2007 Jan 12.


OsYUCCA1 was expressed in almost all of the organs tested, but its expression was restricted to discrete areas, including the tips of leaves, roots, and vascular tissues, where it overlapped with expression of a beta-glucuronidase reporter gene controlled by the auxin-responsive DR5 promoter.
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11967096 Kutz A, Muller A, Hennig P, Kaiser WM, Piotrowski M, Weiler EW: A role for nitrilase 3 in the regulation of root morphology in sulphur-starving Arabidopsis thaliana. Plant J. 2002 Apr;30(1):95-106.


In transgenic plants expressing uidA under the control of the NIT3 promoter (NIT3p::uidA), sulphate deprivation leads to the appearance of beta-glucuronidase activity in shoots and particularly in roots, most strongly in the conductive tissues and lateral root primordia.
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19903871 Strader LC, Beisner ER, Bartel B: Silver ions increase auxin efflux independently of effects on ethylene response. Plant Cell. 2009 Nov;21(11):3585-90. Epub 2009 Nov 10.


We found that although AgNO (3) dramatically decreased root indole-3-acetic acid (IAA) responsiveness in inhibition of root elongation, promotion of DR5-beta-glucuronidase activity, and reduction of Aux/IAA protein levels, AVG had more mild effects.
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17692850 Scherer GF, Zahn M, Callis J, Jones AM: A role for phospholipase A in auxin-regulated gene expression. FEBS Lett. 2007 Sep 4;581(22):4205-11. Epub 2007 Aug 3.


To identify the mode of action, rapid auxin-regulated gene expression was tested for sensitivity to these PLA (2) inhibitors using seedlings expressing beta-glucuronidase (GUS) under the control of the synthetic auxin-responsive promoter DR5.
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19805904 Sharma MK, Solanke AU, Jani D, Singh Y, Sharma AK: A simple and efficient Agrobacterium-mediated procedure for transformation of tomato. J Biosci. 2009 Sep;34(3):423-33.


Transformation of tomato plants was confirmed by Southern blot analysis and beta-glucuronidase (GUS) assay.
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