Protein Information

Name cullin
Synonyms Cullin

Compound Information

Name jasmonic acid
CAS

Reference List

PubMed Abstract RScore(About this table)
15860010 Ren C, Pan J, Peng W, Genschik P, Hobbie L, Hellmann H, Estelle M, Gao B, Peng J, Sun C, Xie D: Point mutations in Arabidopsis Cullin1 reveal its essential role in jasmonate response. Plant J. 2005 May;42(4):514-24.


The SKP1-Cullin/Cdc53-F-box protein ubiquitin ligases (SCF) target many important regulatory proteins for degradation and play vital roles in diverse cellular processes.
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12445118 Devoto A, Nieto-Rostro M, Xie D, Ellis C, Harmston R, Patrick E, Davis J, Sherratt L, Coleman M, Turner JG: COI1 links jasmonate signalling and fertility to the SCF ubiquitin-ligase complex in Arabidopsis. Plant J. 2002 Nov;32(4):457-66.


Other F-box proteins interact with SKP1 and cullin proteins to form SCF complexes that selectively recruit regulatory proteins targeted for ubiquitination.
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11117256 del Pozo JC, Estelle M: F-box proteins and protein degradation: an emerging theme in cellular regulation. Plant Mol Biol. 2000 Sep;44(2):123-8.

In plants, the SCF has so far been implicated in floral development, circadian clock, and response to the plant growth regulators auxin and jasmonic acid.
The SCF is composed of a cullin (Cdc53), SKP1, RBX1 and one member of a large family of proteins called F-box proteins.
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17158585 Moon J, Zhao Y, Dai X, Zhang W, Gray WM, Huq E, Estelle M: A new CULLIN 1 mutant has altered responses to hormones and light in Arabidopsis. Plant Physiol. 2007 Feb;143(2):684-96. Epub 2006 Dec 8.


An analysis of protein interactions with the cul1-6 gene product suggests that both RUB (related to ubiquitin) modification and interaction with the SCF regulatory protein CAND1 (cullin associated and neddylation dissociated) are disrupted.
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20159850 Gfeller A, Liechti R, Farmer EE: Arabidopsis jasmonate signaling pathway. Sci Signal. 2010 Feb 16;3(109):cm4.

The prohormone jasmonic acid (JA) is conjugated to amino acids such as isoleucine to form the active hormone jasmonoyl-isoleucine (JA-Ile).
A series of breakthroughs has identified the SCF [SCF consists of four subunits: a cullin, SKP1 (S-phase kinase-associated protein 1), a RING finger protein (RBX1/HRT1/ROC1), and an F-box protein] CORONATINE INSENSITIVE1 (COI1) E3 ubiquitin ligase complex and the JASMONATE ZIM-DOMAIN (JAZ) proteins as central components in the perception of and transcriptional response to JA-Ile.
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16478048 Wang Z, Dai L, Jiang Z, Peng W, Zhang L, Wang G, Xie D: GmCOI1, a soybean F-box protein gene, shows ability to mediate jasmonate-regulated plant defense and fertility in Arabidopsis. Mol Plant Microbe Interact. 2005 Dec;18(12):1285-95.


Furthermore, GmCOI1 was found to assemble into the Skpl-Cullin-F-box (SCF) complexes, similar to the formation of the Arabidopsis SCF (COO1).
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16492478 Browse J: Jasmonate: an oxylipin signal with many roles in plants. Vitam Horm. 2005;72:431-56.

Currently, the key issues in JA action are to understand the role of the skip/cullin/F-box ubiquitination complex, SCF (COI1), and to identify additional protein components that act in the early steps of JA signaling.
Jasmonic acid is an oxylipin signaling molecule derived from linolenic acid.
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16478935 Gfeller A, Liechti R, Farmer EE: Arabidopsis jasmonate signaling pathway. Sci STKE. 2006 Feb 14;2006(322):cm1.

A series of breakthroughs have identified the SKP/CULLIN/F-BOX (SCF), CORONATINE INSENSITIVE (COI1) complex, acting together with the CONSTITUTIVE PHOTOMORPHOGENIC 9 (COP9) signalosome, as central regulatory components of jasmonate signaling in Arabidopsis.
In male fertility, jasmonic acid (JA) is the essential signal intervening both at the level of anther elongation and in pollen dehiscense.
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12682009 Dharmasiri S, Dharmasiri N, Hellmann H, Estelle M: The RUB/Nedd8 conjugation pathway is required for early development in Arabidopsis. EMBO J. 2003 Apr 15;22(8):1762-70.


The related-to-ubiquitin (RUB) protein is post-translationally conjugated to the cullin subunit of the SCF (SKP1, Cullin, F-box) class of ubiquitin protein ligases.
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19825556 Llorente F, Muskett P, Sanchez-Vallet A, Lopez G, Ramos B, Sanchez-Rodriguez C, Jorda L, Parker J, Molina A: Repression of the auxin response pathway increases Arabidopsis susceptibility to necrotrophic fungi. Mol Plant. 2008 May;1(3):496-509.

In plants, resistance to necrotrophic pathogens depends on the interplay between different hormone systems, such as those regulated by salicylic acid (SA), jasmonic acid (JA), ethylene, and abscisic acid.
Here, we demonstrate that Arabidopsis auxin signaling mutants axr1, axr2, and axr6 that have defects in the auxin-stimulated SCF (Skp1-Cullin-F-box) ubiquitination pathway exhibit increased susceptibility to the necrotrophic fungi Plectosphaerella cucumerina and Botrytis cinerea.
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